Bio Spec Overview
Ignite Creation Date:
2025-12-25 @ 4:49 AM
Last Modification Date:
2025-12-25 @ 4:49 AM
Retention:
SAMPLES_WITH_DNA
Description:
Laboratory assays Dry blood spots were made from capillary blood deposited on Whatman 3 filter paper (serology) and Whatman Protein Saver Card (molecular biology). To document our two main outcomes (HCV and HTLV-1 infections), serological assays were performed as detailed in Web extra material, Appendix. For HCV, PCR amplification of the NS5b (485-nt) and core/E1 (1026-nt) genome regions was attempted on reactive samples. For HTLV-1, the gp21 env gene was amplified (885-nt).
Section:
Laboratory assays Dry blood spots were made from capillary blood deposited on Whatman 3 filter paper (serology) and Whatman Protein Saver Card (molecular biology). To document our two main outcomes (HCV and HTLV-1 infections), serological assays were performed as detailed in Web extra material, Appendix. For HCV, PCR amplification of the NS5b (485-nt) and core/E1 (1026-nt) genome regions was attempted on reactive samples. For HTLV-1, the gp21 env gene was amplified (885-nt).