Ignite Creation Date:
2025-12-24 @ 11:55 PM
Ignite Modification Date:
2025-12-25 @ 9:51 PM
Study NCT ID:
NCT07147751
Status:
NOT_YET_RECRUITING
Last Update Posted:
2025-08-29
First Post:
2025-08-22
Is NOT Gene Therapy:
False
Has Adverse Events:
False
Brief Title:
Characterization of Primary Central Nervous System Diffuse Large B Cell Lymphoma (PCNS-DLBCL) by Multiomic Approach
Sponsor:
Organization:
Raw JSON
{'hasResults': False, 'derivedSection': {'miscInfoModule': {'versionHolder': '2025-12-24'}}, 'protocolSection': {'designModule': {'bioSpec': {'retention': 'SAMPLES_WITH_DNA', 'description': 'Already existing and coded tumor biological material will be retrospectively collected from institutional biobanks upon receipt of ethical approval'}, 'studyType': 'OBSERVATIONAL', 'designInfo': {'timePerspective': 'RETROSPECTIVE', 'observationalModel': 'COHORT'}, 'enrollmentInfo': {'type': 'ESTIMATED', 'count': 100}, 'patientRegistry': False}, 'statusModule': {'overallStatus': 'NOT_YET_RECRUITING', 'startDateStruct': {'date': '2026-04', 'type': 'ESTIMATED'}, 'expandedAccessInfo': {'hasExpandedAccess': False}, 'statusVerifiedDate': '2025-08', 'completionDateStruct': {'date': '2029-12', 'type': 'ESTIMATED'}, 'lastUpdateSubmitDate': '2025-08-22', 'studyFirstSubmitDate': '2025-08-22', 'studyFirstSubmitQcDate': '2025-08-22', 'lastUpdatePostDateStruct': {'date': '2025-08-29', 'type': 'ESTIMATED'}, 'studyFirstPostDateStruct': {'date': '2025-08-29', 'type': 'ESTIMATED'}, 'primaryCompletionDateStruct': {'date': '2029-12', 'type': 'ESTIMATED'}}, 'outcomesModule': {'primaryOutcomes': [{'measure': 'Prevalence of non-GC phenotype', 'timeFrame': '12 months: from the end of samples collection to the end of study analysis'}, {'measure': 'Prevalence of mutations, copy number abnormalities, and structural variants', 'timeFrame': '12 months: from the end of samples collection to the end of study analysis'}, {'measure': 'Prevalence of PCNS-DLBCL molecular subtypes defined by genetic lesions', 'timeFrame': '12 months: from the end of samples collection to the end of study analysis'}, {'measure': 'Prevalence of PCNS-DLBCL molecular subtypes defined by gene expression signatures', 'timeFrame': '12 months: from the end of samples collection to the end of study analysis'}, {'measure': 'Prevalence of PCNS-DLBCL molecular subtypes defined by genetic methylation profile', 'timeFrame': '12 months: from the end of samples collection to the end of study analysis'}], 'secondaryOutcomes': [{'measure': 'Prevalence of molecular features of clinical, laboratory, and radiological features', 'timeFrame': '12 months: from the end of samples collection to the end of study analysis'}, {'measure': 'Prevalence of PCNS-DLBCL immunological signatures defined by gene expression profiling', 'timeFrame': '12 months: from the end of samples collection to the end of study analysis'}, {'measure': '3. Prevalence of PCNS-DLBCL MicroRNA signatures defined by miRNOMA evaluation', 'timeFrame': '12 months: from the end of samples collection to the end of study analysis'}]}, 'oversightModule': {'oversightHasDmc': False, 'isFdaRegulatedDrug': False, 'isFdaRegulatedDevice': False}, 'conditionsModule': {'conditions': ['Primary Central Nervous System Diffuse Large B Cell Lymphoma (PCNS-DLBCL)']}, 'descriptionModule': {'briefSummary': 'PCNS-DLBCL is a rare extranodal non-Hodgkin lymphoma that primarily affects the brain, spine, or vitreoretinal space. The prognosis for PCNS-DLBCL is significantly worse than that for its systemic counterpart.\n\nUnderstanding how and where this tumor initiates, and how it survives or depends on the microenvironment of the CNS is key to understanding the underlying biology and identifying reliable biomarkers for selecting personalized therapy or for biologically directed therapy that could improve the cure rate of CNS lymphomas.', 'detailedDescription': "Already existing and coded tumor biological material and health-related patient data will be retrospectively collected from institutional biobanks and patients' medical charts or electronic medical records upon receipt of ethical approval. Each patient enrolled in the study will be assigned a unique identification numerical code upon registration in the study. The unique identification code will be used to record health-related data and to label biological samples. The coded biological material will be transferred at the Institute of Oncology Research in Bellinzona and the IRCCS Policlinico San Matteo Foundation in Pavia.\n\nDiagnostic slides will be centrally revised by a panel of expert pathologists to confirm the diagnosis of PCNS-DLBCL. Health-related data will be collected in the e-CRFs. Data quality will be ensured by query generation.\n\nAnnotated baseline features will include: age, sex, the date of diagnosis, ECOG PS, IELSG index, clinical features (i.e. neurologic signs or symptoms, psychiatric and behavioural changes, systemic lymphoma symptoms, and comorbidities), imaging features (i.e. MRI report), disease involvements (i.e. bone marrow infiltrate, CSF, Vitro-retinal, basal ganglia, corpus callosum, brain stem, or cerebellum), laboratory features (i.e. complete blood count parameters, LDH levels, HIV infection evidence, HCV infection evidence, HBV infection evidence, EBV infection evidence, CMV infection evidence, and cerebrospinal fluid examination parameters).\n\nAnnotated follow-up features will include: the start date, the regimen, the response level and the date of response evaluation of the first-line therapy, the start date, the regimen, the response level and the date of response evaluation of the second-line therapy; the date of transplantation and the date of relapse.\n\nSurvival features will include date of death, cause of death, and date of last follow-up.\n\nAll FFPE samples will undergo a comprehensive histological review, including immunohistochemical investigations aimed to analyze the various cell populations and the microenvironment; EBER ISH assay to verify the presence of latent EBV-infection, FISH assays to verify the presence of translocations involving BCL2, BCL6, and MYC genes, and of CDKN2A and 6p21-22 (HLA locus) loss.\n\nMolecular analyses on tumor samples will include: 1) DNA alteration analysis by means of NGS approach; 2) genome-wide methylation profiling (the sole epigenetic fingerprints that can be robustly assayed on FFPE material); 3) transcriptional profiling; 4) Ig heavy chain (IGH) rearrangements detection and immunoglobulin heavy-chain variable region gene (IGHV) analysis.\n\nThe results of all these analyses will be recorded in the clinical-biological database. Statistical analysis will be performed to integrate and correlate obtained biological data with collected radiomic and clinical information, to outline a precise PCNS-DLBCL multiomic profile and to investigate unraveled genes or cellular pathways, potentially useful for diagnostic, predictive, and therapeutic purposes. Hierarchical clustering analysis will be performed to identify genetic driver alterations and discover any subsets of tumors with distinctive multiomic (genetic/transcriptomic/radiomic) signatures."}, 'eligibilityModule': {'sex': 'ALL', 'stdAges': ['ADULT', 'OLDER_ADULT'], 'minimumAge': '18 Years', 'samplingMethod': 'PROBABILITY_SAMPLE', 'studyPopulation': 'Adults patients with primary central nervous system diffuse large B cell lymphoma (PCNS-DLBCL)', 'healthyVolunteers': False, 'eligibilityCriteria': 'Inclusion Criteria:\n\n1. Be an adult immunocompetent patient who received a PCNS-DLBCL diagnosis according to the WHO-HAEM5 criteria between 2018 and 2024.\n2. Availability of formalin-fixed paraffin-embedded (FFPE) diagnostic tumor material.\n3. Availability of the baseline and follow-up annotations\n\nExclusion Criteria:\n\n1. Established immunodeficiency conditions.\n2. Secondary CNS localizations of DLBCL\n3. Transformed DLBCL'}, 'identificationModule': {'nctId': 'NCT07147751', 'briefTitle': 'Characterization of Primary Central Nervous System Diffuse Large B Cell Lymphoma (PCNS-DLBCL) by Multiomic Approach', 'organization': {'class': 'OTHER', 'fullName': 'International Extranodal Lymphoma Study Group (IELSG)'}, 'officialTitle': 'Characterization of Primary Central Nervous System Diffuse Large B Cell Lymphoma (PCNS-DLBCL) by Multiomic Approach', 'orgStudyIdInfo': {'id': 'IELSG57'}}, 'contactsLocationsModule': {'locations': [{'zip': '27100', 'city': 'Pavia', 'country': 'Italy', 'contacts': [{'name': 'Marco Lucioni, MD', 'role': 'CONTACT', 'email': 'marco.lucioni@unipv.it'}, {'name': 'Marco Lucioni, MD', 'role': 'PRINCIPAL_INVESTIGATOR'}], 'facility': 'Fondazione IRCCS Policlinico San Matteo Pavia', 'geoPoint': {'lat': 45.19205, 'lon': 9.15917}}, {'zip': '6500', 'city': 'Bellinzona', 'country': 'Switzerland', 'contacts': [{'name': 'Maria Cristina Pirosa, MD', 'role': 'CONTACT', 'email': 'maria.pirosa@eoc.ch'}, {'name': 'Maria Cristina Pirosa, MD', 'role': 'PRINCIPAL_INVESTIGATOR'}], 'facility': 'Oncology Institute of Southern Switzerland and Institute of Oncology Research', 'geoPoint': {'lat': 46.19278, 'lon': 9.01703}}], 'centralContacts': [{'name': 'International Extranodal Lymphoma Study Group - IELSG', 'role': 'CONTACT', 'email': 'ielsg@ior.usi.ch', 'phone': '+41 58 666 7321'}], 'overallOfficials': [{'name': 'Marco Paulli, MD', 'role': 'STUDY_CHAIR', 'affiliation': 'Unit of Pathology, IRCCS Policlinico San Matteo Foundation and Department of Molecular Medicine, University of Pavia (IT)'}, {'name': 'Marco Lucioni, MD', 'role': 'STUDY_CHAIR', 'affiliation': 'Unit of Pathology, IRCCS Policlinico San Matteo Foundation and Department of Molecular Medicine, University of Pavia (IT)'}]}, 'ipdSharingStatementModule': {'ipdSharing': 'NO'}, 'sponsorCollaboratorsModule': {'leadSponsor': {'name': 'International Extranodal Lymphoma Study Group (IELSG)', 'class': 'OTHER'}, 'responsibleParty': {'type': 'SPONSOR'}}}}