Ignite Creation Date:
2025-12-26 @ 10:46 PM
Ignite Modification Date:
2025-12-26 @ 10:46 PM
Study NCT ID:
NCT01054612
Status:
WITHDRAWN
Last Update Posted:
2015-03-04
First Post:
2007-12-21
Is NOT Gene Therapy:
False
Has Adverse Events:
False
Brief Title:
Tissue Sectioning by Electro-Dissociation
Sponsor:
Organization:
Raw JSON
{'hasResults': False, 'derivedSection': {'miscInfoModule': {'versionHolder': '2025-12-24'}}, 'protocolSection': {'designModule': {'studyType': 'OBSERVATIONAL', 'enrollmentInfo': {'type': 'ACTUAL', 'count': 0}}, 'statusModule': {'whyStopped': 'The study was closed due to the departure of several of the co-investigators.', 'overallStatus': 'WITHDRAWN', 'startDateStruct': {'date': '2002-03'}, 'expandedAccessInfo': {'hasExpandedAccess': False}, 'statusVerifiedDate': '2015-03', 'completionDateStruct': {'date': '2010-01', 'type': 'ACTUAL'}, 'lastUpdateSubmitDate': '2015-03-03', 'studyFirstSubmitDate': '2007-12-21', 'studyFirstSubmitQcDate': '2010-01-20', 'lastUpdatePostDateStruct': {'date': '2015-03-04', 'type': 'ESTIMATED'}, 'studyFirstPostDateStruct': {'date': '2010-01-22', 'type': 'ESTIMATED'}, 'primaryCompletionDateStruct': {'date': '2010-01', 'type': 'ACTUAL'}}, 'conditionsModule': {'keywords': ['Histology', 'Immunohistochemical', 'Gene Expression', 'Tissue Sectioning'], 'conditions': ['Thin Sections', 'Fresh Unfixed Tissues', 'Fresh Unfrozen Tissues']}, 'descriptionModule': {'briefSummary': 'Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).'}, 'eligibilityModule': {'sex': 'ALL', 'stdAges': ['CHILD', 'ADULT', 'OLDER_ADULT'], 'samplingMethod': 'PROBABILITY_SAMPLE', 'studyPopulation': 'Discarded human tissue obtained immediately following surgical resection', 'healthyVolunteers': False, 'eligibilityCriteria': 'Inclusion Criteria:\n\n* Fresh Tissue\n* Unfrozen Tissue\n* Unfixed Tissue\n\nExclusion Criteria:\n\n* Unfresh Tissue\n* Frozen Tissue\n* Fixed Tissue'}, 'identificationModule': {'nctId': 'NCT01054612', 'briefTitle': 'Tissue Sectioning by Electro-Dissociation', 'organization': {'class': 'OTHER', 'fullName': 'University of Arkansas'}, 'officialTitle': 'Tissue Sectioning by Electro-Dissociation', 'orgStudyIdInfo': {'id': 'IRB 28177'}, 'secondaryIdInfos': [{'id': 'NBIB'}]}, 'armsInterventionsModule': {'interventions': [{'name': 'Tissue Sectioning via Electro Erosion Process', 'type': 'OTHER', 'description': 'Focused RF energy can be used to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analysis by electro-dissociation without thermal damage, ultimately allowing high resolution reconstruction of gene and protein expression patterns of large tissue specimens in 3D.'}]}, 'contactsLocationsModule': {'overallOfficials': [{'name': 'Gal Shafirstein, Ph.D.', 'role': 'PRINCIPAL_INVESTIGATOR', 'affiliation': 'UAMS, ACH, Central Arkansas Veterans Healthcare System'}]}, 'sponsorCollaboratorsModule': {'leadSponsor': {'name': 'University of Arkansas', 'class': 'OTHER'}, 'responsibleParty': {'oldNameTitle': 'Dr. Gal Shafirstein', 'oldOrganization': "University of Arkansas, Arkansas Children's Hospital"}}}}