Ignite Creation Date:
2025-12-24 @ 12:04 PM
Ignite Modification Date:
2025-12-30 @ 3:18 AM
Study NCT ID:
NCT05814861
Status:
UNKNOWN
Last Update Posted:
2023-04-18
First Post:
2023-03-20
Is Gene Therapy:
True
Has Adverse Events:
False
Brief Title:
Isolation and Characterization of Blastocystis Hominis as Apotential Human Pathogen
Sponsor:
Organization:
Raw JSON
{'hasResults': False, 'derivedSection': {'miscInfoModule': {'versionHolder': '2025-12-24'}, 'conditionBrowseModule': {'meshes': [{'id': 'D016776', 'term': 'Blastocystis Infections'}], 'ancestors': [{'id': 'D007411', 'term': 'Intestinal Diseases, Parasitic'}, {'id': 'D010272', 'term': 'Parasitic Diseases'}, {'id': 'D007239', 'term': 'Infections'}, {'id': 'D000562', 'term': 'Amebiasis'}, {'id': 'D011528', 'term': 'Protozoan Infections'}, {'id': 'D007410', 'term': 'Intestinal Diseases'}, {'id': 'D005767', 'term': 'Gastrointestinal Diseases'}, {'id': 'D004066', 'term': 'Digestive System Diseases'}]}}, 'protocolSection': {'designModule': {'studyType': 'OBSERVATIONAL', 'designInfo': {'timePerspective': 'CROSS_SECTIONAL', 'observationalModel': 'CASE_CONTROL'}, 'enrollmentInfo': {'type': 'ESTIMATED', 'count': 72}, 'patientRegistry': False}, 'statusModule': {'overallStatus': 'UNKNOWN', 'lastKnownStatus': 'NOT_YET_RECRUITING', 'startDateStruct': {'date': '2023-12-01', 'type': 'ESTIMATED'}, 'expandedAccessInfo': {'hasExpandedAccess': False}, 'statusVerifiedDate': '2023-03', 'completionDateStruct': {'date': '2024-12-31', 'type': 'ESTIMATED'}, 'lastUpdateSubmitDate': '2023-04-14', 'studyFirstSubmitDate': '2023-03-20', 'studyFirstSubmitQcDate': '2023-04-14', 'lastUpdatePostDateStruct': {'date': '2023-04-18', 'type': 'ACTUAL'}, 'studyFirstPostDateStruct': {'date': '2023-04-18', 'type': 'ACTUAL'}, 'primaryCompletionDateStruct': {'date': '2024-10-01', 'type': 'ESTIMATED'}}, 'outcomesModule': {'primaryOutcomes': [{'measure': '-Morphological identification of Blastocystis hominis in stool.', 'timeFrame': '1year', 'description': 'Microscopic Detection of Blastocystis hominis among outpatients presented with GIT symptoms at Assiut University Hospitals .'}], 'secondaryOutcomes': [{'measure': 'Molecular identification of Blastocystis hominis', 'timeFrame': '1 year', 'description': 'Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).'}]}, 'oversightModule': {'isFdaRegulatedDrug': False, 'isFdaRegulatedDevice': False}, 'conditionsModule': {'conditions': ['Blastocystis Hominis Infections']}, 'referencesModule': {'references': [{'type': 'BACKGROUND', 'citation': 'A. F. Bafghi, R. Hosseini, H. R. Mollaei, and K. Barzegar, "Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman , Iran," vol. 8, no. 01, pp. 10-16, 2021..'}, {'type': 'BACKGROUND', 'citation': 'S M. Journal, "SOHAG MEDICAL JOURNAL Vol. 21 No.3," vol. 21, no. 3, 2017.'}, {'type': 'BACKGROUND', 'citation': 'M. Q. Khairuzzaman, "No Title血清及尿液特定蛋白检测在糖尿病肾病早期诊断中的意义," vol. 4, no. 1, pp. 64-75, 2016.'}]}, 'descriptionModule': {'briefSummary': '* Detection of the presence of Blastocystis hominis among outpatients presented with GIT symptoms at Assiut University Hospitals in comparison with those in control group.\n* Assessment of different culture techniques for detection, and isolation of Blastocystis hominis.\n* Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).', 'detailedDescription': 'Blastocystis is a genus of single-celled parasites belonging to a group of organisms that are known as the Stramenopiles . Blastocystis consists of several species, living in the gastrointestinal tracts of humans and farm animals. Many different species can infect humans and would be identified as Blastocystis hominis depending on the sequencing of the gene encoding the 18S rRNA . Among the subtypes recognized, ST1-8 could colonize humans as well as animals.\n\nInfection with B. hominis has a worldwide distribution with infection rate reaches up to 60% in developing countries. Initially B.hominis was accepted as a harmless commensal in the intestinal tract, but recently it was implicated as a pathogen. As it able to produce a cysteine protease which breaks up the mucosal IgA antibody. B.hominis infection has been associated with several gastrointestinal symptoms, Even though most cases are asymptomatic, it is also play a significant role in some chronic gastrointestinal illnesses as irritable bowel syndrome (IBS).\n\nBlastocystis spp. are polymorphic parasites with multiple morphological forms including vacuolar, multivacuolar, granular, ameboid, and cystic forms. At least two out of three infections may be missed during microscopic detection of B.hominis due to their polymorphic nature, and the possibility of confusion with yeast, Cyclospora spp. or fat globules. . Different studies suggested that it multiply rapidly in culture medium and considered it a sensitive method for diagnosis of B.hominis . Recently Molecular diagnosis by polymerase chain reaction (PCR) is considered the most sensitive method for the detection of B.hominis.'}, 'eligibilityModule': {'sex': 'ALL', 'stdAges': ['CHILD', 'ADULT', 'OLDER_ADULT'], 'samplingMethod': 'NON_PROBABILITY_SAMPLE', 'studyPopulation': 'The persons selected for the study are those who are:\n\n1. Suffering from any GIT manifestations, attending outpatient clinics of Internal Medicine and Tropical Medicine Departments at Assiut University Hospitals (case group)\n\n * GIT manifestations such as diarrhea; (frequency ≥3 loose stool /day), either acute diarrhea (duration less than 2 weeks), or chronic diarrhea (duration more than 4 weeks), or other GIT complaints as abdominal pain, flatulence, nausea, vomiting, or weight loss.\n2. clinically free persons undergo routine stool analysis for any reasons other than GIT complaint.(control group)', 'eligibilityCriteria': 'Inclusion Criteria:\n\n* The persons selected for the study are those who are:\n\n 1. Suffering from any GIT manifestations, attending outpatient clinics of Internal Medicine and Tropical Medicine Departments at Assiut University Hospitals (case group)\n 2. clinically free persons undergo routine stool analysis for any reasons other than GIT complaint.(control group)\n\nExclusion Criteria:\n\n* We will exclude any patient who received antibiotic or anti-parasitic drugs the past 4 weeks prior to sample collection.'}, 'identificationModule': {'nctId': 'NCT05814861', 'briefTitle': 'Isolation and Characterization of Blastocystis Hominis as Apotential Human Pathogen', 'organization': {'class': 'OTHER', 'fullName': 'Assiut University'}, 'officialTitle': 'Isolation and Characterization of Blastocystis Hominis as Apotential Human Pathogen', 'orgStudyIdInfo': {'id': 'Blastocystis hominis isolation'}}, 'armsInterventionsModule': {'armGroups': [{'label': 'patients presented with GIT symptoms', 'description': 'Fresh Stool samples will be collected in clean sterile cups from patients suffering from gastrointestinal symptoms attending Assiut University Hospitals and examined for detection of Blastocystis hominis', 'interventionNames': ['Diagnostic Test: culture and PCR']}, {'label': 'clinically free persons undergo routine stool analysis for any reasons other than GIT complaint', 'description': 'Fresh Stool samples will be collected in clean sterile cups from clinically free persons under go routine stool examination for any cause other than GIT troubles and examined for detection of Blastocystis hominis', 'interventionNames': ['Diagnostic Test: culture and PCR']}], 'interventions': [{'name': 'culture and PCR', 'type': 'DIAGNOSTIC_TEST', 'description': "Stool samples will be cultured inTwo media :\n\nA-Locke egg serum medium (LE) B- Jones' medium Then Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).", 'armGroupLabels': ['clinically free persons undergo routine stool analysis for any reasons other than GIT complaint', 'patients presented with GIT symptoms']}]}, 'contactsLocationsModule': {'centralContacts': [{'name': 'Fatma Alzahraa montaser Ali, demonstrator', 'role': 'CONTACT', 'email': 'docfatma490@gmail.com', 'phone': '01060093655'}, {'name': 'magda mohamed atiya Elnazer, Prof.', 'role': 'CONTACT', 'email': 'Magda.elnazer@med.aun.edu.eg', 'phone': '01143533100'}]}, 'ipdSharingStatementModule': {'ipdSharing': 'NO'}, 'sponsorCollaboratorsModule': {'leadSponsor': {'name': 'Assiut University', 'class': 'OTHER'}, 'responsibleParty': {'type': 'PRINCIPAL_INVESTIGATOR', 'investigatorTitle': 'Investigator', 'investigatorFullName': 'Fatma Alzahraa Montaser Ali', 'investigatorAffiliation': 'Assiut University'}}}}