Ignite Creation Date:
2025-12-25 @ 1:59 AM
Ignite Modification Date:
2026-02-20 @ 10:56 PM
Study NCT ID:
NCT02935894
Status:
COMPLETED
Last Update Posted:
2018-06-01
First Post:
2016-10-12
Is NOT Gene Therapy:
True
Has Adverse Events:
True
Brief Title:
Investigating the Stability, Variability and Mechanism of Incorporation of Lipid Mediators Into Eccrine Sweat
Sponsor:
Organization:
Raw JSON
{'hasResults': True, 'derivedSection': {'miscInfoModule': {'versionHolder': '2025-12-24'}, 'interventionBrowseModule': {'meshes': [{'id': 'D007052', 'term': 'Ibuprofen'}, {'id': 'D015444', 'term': 'Exercise'}, {'id': 'D010862', 'term': 'Pilocarpine'}], 'ancestors': [{'id': 'D010666', 'term': 'Phenylpropionates'}, {'id': 'D000146', 'term': 'Acids, Carbocyclic'}, {'id': 'D002264', 'term': 'Carboxylic Acids'}, {'id': 'D009930', 'term': 'Organic Chemicals'}, {'id': 'D009043', 'term': 'Motor Activity'}, {'id': 'D009068', 'term': 'Movement'}, {'id': 'D009142', 'term': 'Musculoskeletal Physiological Phenomena'}, {'id': 'D055687', 'term': 'Musculoskeletal and Neural Physiological Phenomena'}, {'id': 'D000470', 'term': 'Alkaloids'}, {'id': 'D006571', 'term': 'Heterocyclic Compounds'}]}}, 'resultsSection': {'moreInfoModule': {'pointOfContact': {'email': 'john.newman@ars.usda.gov', 'phone': '530-752-1009', 'title': 'Dr. John W. Newman', 'organization': 'USDA, ARS Western Human Nutrition Research Center'}, 'certainAgreement': {'piSponsorEmployee': True}}, 'adverseEventsModule': {'timeFrame': 'Adverse events collected from November 2016 to June 2017 (6 months)', 'eventGroups': [{'id': 'EG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine', 'otherNumAtRisk': 14, 'deathsNumAtRisk': 14, 'otherNumAffected': 0, 'seriousNumAtRisk': 14, 'deathsNumAffected': 0, 'seriousNumAffected': 0}], 'frequencyThreshold': '0'}, 'outcomeMeasuresModule': {'outcomeMeasures': [{'type': 'PRIMARY', 'title': 'Volar Forearm Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation', 'denoms': [{'units': 'Participants', 'counts': [{'value': '7', 'groupId': 'OG000'}]}], 'groups': [{'id': 'OG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'classes': [{'title': '9-HODE', 'categories': [{'measurements': [{'value': '20', 'spread': '9.8', 'groupId': 'OG000'}]}]}, {'title': '13-HODE', 'categories': [{'measurements': [{'value': '46', 'spread': '28', 'groupId': 'OG000'}]}]}, {'title': '9-HOTrE', 'categories': [{'measurements': [{'value': '2.2', 'spread': '1.2', 'groupId': 'OG000'}]}]}, {'title': '13-HOTrE', 'categories': [{'measurements': [{'value': '3.5', 'spread': '1.8', 'groupId': 'OG000'}]}]}, {'title': '5-HETE', 'categories': [{'measurements': [{'value': '0.1', 'spread': '0.07', 'groupId': 'OG000'}]}]}, {'title': '8-HETE', 'categories': [{'measurements': [{'value': '0.23', 'spread': '0.062', 'groupId': 'OG000'}]}]}, {'title': '9-HETE', 'categories': [{'measurements': [{'value': '0.12', 'spread': '0.062', 'groupId': 'OG000'}]}]}, {'title': '11-HETE', 'categories': [{'measurements': [{'value': '0.15', 'spread': '0.055', 'groupId': 'OG000'}]}]}, {'title': '12-HETE', 'categories': [{'measurements': [{'value': '0.85', 'spread': '0.94', 'groupId': 'OG000'}]}]}, {'title': '15-HETE', 'categories': [{'measurements': [{'value': '0.43', 'spread': '0.25', 'groupId': 'OG000'}]}]}, {'title': '12-HEPE', 'categories': [{'measurements': [{'value': '0.086', 'spread': '0.044', 'groupId': 'OG000'}]}]}, {'title': '9,10-e-DiHO', 'categories': [{'measurements': [{'value': '85', 'spread': '37', 'groupId': 'OG000'}]}]}, {'title': '9,10-DiHOME', 'categories': [{'measurements': [{'value': '4.4', 'spread': '3', 'groupId': 'OG000'}]}]}, {'title': '12,13-DiHOME', 'categories': [{'measurements': [{'value': '3.4', 'spread': '2.3', 'groupId': 'OG000'}]}]}, {'title': '9,10-DiHODE', 'categories': [{'measurements': [{'value': '0.85', 'spread': '1.1', 'groupId': 'OG000'}]}]}, {'title': '12,13-DiHODE', 'categories': [{'measurements': [{'value': '0.5', 'spread': '0.31', 'groupId': 'OG000'}]}]}, {'title': '15,16-DiHODE', 'categories': [{'measurements': [{'value': '2.7', 'spread': '3.9', 'groupId': 'OG000'}]}]}, {'title': '5,6-DiHETrE', 'categories': [{'measurements': [{'value': '5.3', 'spread': '2.5', 'groupId': 'OG000'}]}]}, {'title': '8,9-DiHETrE', 'categories': [{'measurements': [{'value': '0.73', 'spread': '0.43', 'groupId': 'OG000'}]}]}, {'title': '11,12-DiHETrE', 'categories': [{'measurements': [{'value': '0.51', 'spread': '0.29', 'groupId': 'OG000'}]}]}, {'title': '14,15-DiHETrE', 'categories': [{'measurements': [{'value': '0.65', 'spread': '0.4', 'groupId': 'OG000'}]}]}, {'title': '9,10-EpOME', 'categories': [{'measurements': [{'value': '1.7', 'spread': '0.79', 'groupId': 'OG000'}]}]}, {'title': '12,13-EpOME', 'categories': [{'measurements': [{'value': '0.96', 'spread': '0.29', 'groupId': 'OG000'}]}]}, {'title': '9,10-EpODE', 'categories': [{'measurements': [{'value': '0.55', 'spread': '0.54', 'groupId': 'OG000'}]}]}, {'title': '12,13-EpODE', 'categories': [{'measurements': [{'value': '0.12', 'spread': '0.066', 'groupId': 'OG000'}]}]}, {'title': '15,16-EpODE', 'categories': [{'measurements': [{'value': '3.5', 'spread': '3.4', 'groupId': 'OG000'}]}]}, {'title': '9-KODE', 'categories': [{'measurements': [{'value': '14', 'spread': '6.3', 'groupId': 'OG000'}]}]}, {'title': '13-KODE', 'categories': [{'measurements': [{'value': '7.2', 'spread': '3.8', 'groupId': 'OG000'}]}]}, {'title': '12,13-Ep-9-KODE', 'categories': [{'measurements': [{'value': '3.7', 'spread': '2.8', 'groupId': 'OG000'}]}]}, {'title': '5-KETE', 'categories': [{'measurements': [{'value': '0.025', 'spread': '0.019', 'groupId': 'OG000'}]}]}, {'title': 'LTB5', 'categories': [{'measurements': [{'value': '0.1', 'spread': '0.094', 'groupId': 'OG000'}]}]}, {'title': '10-Nitrooleate', 'categories': [{'measurements': [{'value': '0.096', 'spread': '0.054', 'groupId': 'OG000'}]}]}, {'title': '10-Nitrolinoleate', 'categories': [{'measurements': [{'value': '0.11', 'spread': '0.069', 'groupId': 'OG000'}]}]}, {'title': 'PGE1', 'categories': [{'measurements': [{'value': '0.24', 'spread': '0.15', 'groupId': 'OG000'}]}]}, {'title': 'PGE2', 'categories': [{'measurements': [{'value': '2.1', 'spread': '1.8', 'groupId': 'OG000'}]}]}, {'title': 'PGF2a', 'categories': [{'measurements': [{'value': '0.14', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': '15-deoxy PGJ2', 'categories': [{'measurements': [{'value': '0.033', 'spread': '0.012', 'groupId': 'OG000'}]}]}, {'title': 'PGE3', 'categories': [{'measurements': [{'value': '0.13', 'spread': '0.17', 'groupId': 'OG000'}]}]}, {'title': 'Sum TriHOMEs', 'categories': [{'measurements': [{'value': '57', 'spread': '56', 'groupId': 'OG000'}]}]}, {'title': 'PEA', 'categories': [{'measurements': [{'value': '7.9', 'spread': '6.8', 'groupId': 'OG000'}]}]}, {'title': 'SEA', 'categories': [{'measurements': [{'value': '7.9', 'spread': '6.7', 'groupId': 'OG000'}]}]}, {'title': 'OEA', 'categories': [{'measurements': [{'value': '0.32', 'spread': '0.38', 'groupId': 'OG000'}]}]}, {'title': 'LEA', 'categories': [{'measurements': [{'value': '0.043', 'spread': '0.035', 'groupId': 'OG000'}]}]}, {'title': 'aLEA', 'categories': [{'measurements': [{'value': '0.0047', 'spread': '0.0036', 'groupId': 'OG000'}]}]}, {'title': 'Dihomo GLA EA', 'categories': [{'measurements': [{'value': '0.0022', 'spread': '0.003', 'groupId': 'OG000'}]}]}, {'title': 'AEA', 'categories': [{'measurements': [{'value': '0.019', 'spread': '0.011', 'groupId': 'OG000'}]}]}, {'title': 'DEA', 'categories': [{'measurements': [{'value': '0.17', 'spread': '0.24', 'groupId': 'OG000'}]}]}, {'title': '1-OG', 'categories': [{'measurements': [{'value': '870', 'spread': '1300', 'groupId': 'OG000'}]}]}, {'title': '2-OG', 'categories': [{'measurements': [{'value': '190', 'spread': '270', 'groupId': 'OG000'}]}]}, {'title': '1-LG', 'categories': [{'measurements': [{'value': '300', 'spread': '540', 'groupId': 'OG000'}]}]}, {'title': '2-LG', 'categories': [{'measurements': [{'value': '2.3', 'spread': '1.1', 'groupId': 'OG000'}]}]}, {'title': '1-AG', 'categories': [{'measurements': [{'value': '14', 'spread': '12', 'groupId': 'OG000'}]}]}, {'title': '2-AG', 'categories': [{'measurements': [{'value': '15', 'spread': '30', 'groupId': 'OG000'}]}]}, {'title': 'NO-Gly', 'categories': [{'measurements': [{'value': '0.48', 'spread': '0.22', 'groupId': 'OG000'}]}]}, {'title': 'NA-Gly', 'categories': [{'measurements': [{'value': '0.017', 'spread': '0.02', 'groupId': 'OG000'}]}]}, {'title': 'C14 Ceramide', 'categories': [{'measurements': [{'value': '1.4', 'spread': '1.1', 'groupId': 'OG000'}]}]}, {'title': 'C16 Ceramide', 'categories': [{'measurements': [{'value': '6.6', 'spread': '4.7', 'groupId': 'OG000'}]}]}, {'title': 'C18 Ceramide', 'categories': [{'measurements': [{'value': '1.2', 'spread': '0.66', 'groupId': 'OG000'}]}]}, {'title': 'C20 Ceramide', 'categories': [{'measurements': [{'value': '2', 'spread': '1.4', 'groupId': 'OG000'}]}]}, {'title': 'C24 Ceramide', 'categories': [{'measurements': [{'value': '5.7', 'spread': '4.9', 'groupId': 'OG000'}]}]}, {'title': '18:1 Sphingosine', 'categories': [{'measurements': [{'value': '15', 'spread': '8.9', 'groupId': 'OG000'}]}]}], 'paramType': 'MEAN', 'timeFrame': 'measured at study visit 1, 2 and 3; detected lipid mediator concentrations for study visit 1 reported', 'description': 'Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids.', 'unitOfMeasure': 'picomoles per milliliter', 'dispersionType': 'Standard Deviation', 'reportingStatus': 'POSTED', 'populationDescription': 'Of the 14 subjects recruited for this study, only 7 provided usable data for this primary outcome.'}, {'type': 'PRIMARY', 'title': 'Volar Forearm Sweat Lipid Mediator Concentrations Following Exercise', 'denoms': [{'units': 'Participants', 'counts': [{'value': '7', 'groupId': 'OG000'}]}], 'groups': [{'id': 'OG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'classes': [{'title': '9-HODE', 'categories': [{'measurements': [{'value': '2.4', 'spread': '1.9', 'groupId': 'OG000'}]}]}, {'title': '13-HODE', 'categories': [{'measurements': [{'value': '5.2', 'spread': '3.9', 'groupId': 'OG000'}]}]}, {'title': '9-HOTrE', 'categories': [{'measurements': [{'value': '0.6', 'spread': '0.5', 'groupId': 'OG000'}]}]}, {'title': '13-HOTrE', 'categories': [{'measurements': [{'value': '1.5', 'spread': '1.4', 'groupId': 'OG000'}]}]}, {'title': '5-HETE', 'categories': [{'measurements': [{'value': '0.023', 'spread': '0.018', 'groupId': 'OG000'}]}]}, {'title': '8-HETE', 'categories': [{'measurements': [{'value': '0.14', 'spread': '0.061', 'groupId': 'OG000'}]}]}, {'title': '9-HETE', 'categories': [{'measurements': [{'value': '0.029', 'spread': '0.018', 'groupId': 'OG000'}]}]}, {'title': '11-HETE', 'categories': [{'measurements': [{'value': '0.012', 'spread': '0.0061', 'groupId': 'OG000'}]}]}, {'title': '12-HETE', 'categories': [{'measurements': [{'value': '0.04', 'spread': '0.031', 'groupId': 'OG000'}]}]}, {'title': '15-HETE', 'categories': [{'measurements': [{'value': '0.074', 'spread': '0.054', 'groupId': 'OG000'}]}]}, {'title': '12-HEPE', 'categories': [{'measurements': [{'value': '0.029', 'spread': '0.015', 'groupId': 'OG000'}]}]}, {'title': '9,10-e-DiHO', 'categories': [{'measurements': [{'value': '21', 'spread': '12', 'groupId': 'OG000'}]}]}, {'title': '9,10-DiHOME', 'categories': [{'measurements': [{'value': '3.1', 'spread': '1.8', 'groupId': 'OG000'}]}]}, {'title': '12,13-DiHOME', 'categories': [{'measurements': [{'value': '2.8', 'spread': '1.6', 'groupId': 'OG000'}]}]}, {'title': '9,10-DiHODE', 'categories': [{'measurements': [{'value': '0.56', 'spread': '0.68', 'groupId': 'OG000'}]}]}, {'title': '12,13-DiHODE', 'categories': [{'measurements': [{'value': '0.3', 'spread': '0.12', 'groupId': 'OG000'}]}]}, {'title': '15,16-DiHODE', 'categories': [{'measurements': [{'value': '1.9', 'spread': '2.3', 'groupId': 'OG000'}]}]}, {'title': '5,6-DiHETrE', 'categories': [{'measurements': [{'value': '0.026', 'spread': '0.037', 'groupId': 'OG000'}]}]}, {'title': '8,9-DiHETrE', 'categories': [{'measurements': [{'value': '0.24', 'spread': '0.25', 'groupId': 'OG000'}]}]}, {'title': '11,12-DiHETrE', 'categories': [{'measurements': [{'value': '0.022', 'spread': '0.026', 'groupId': 'OG000'}]}]}, {'title': '14,15-DiHETrE', 'categories': [{'measurements': [{'value': '0.041', 'spread': '0.018', 'groupId': 'OG000'}]}]}, {'title': '9,10-EpOME', 'categories': [{'measurements': [{'value': '0.46', 'spread': '0.31', 'groupId': 'OG000'}]}]}, {'title': '12,13-EpOME', 'categories': [{'measurements': [{'value': '0.36', 'spread': '0.22', 'groupId': 'OG000'}]}]}, {'title': '9,10-EpODE', 'categories': [{'measurements': [{'value': '0.19', 'spread': '0.18', 'groupId': 'OG000'}]}]}, {'title': '12,13-EpODE', 'categories': [{'measurements': [{'value': '0.019', 'spread': '0.014', 'groupId': 'OG000'}]}]}, {'title': '15,16-EpODE', 'categories': [{'measurements': [{'value': '0.82', 'spread': '0.86', 'groupId': 'OG000'}]}]}, {'title': '9-KODE', 'categories': [{'measurements': [{'value': '1.5', 'spread': '1.4', 'groupId': 'OG000'}]}]}, {'title': '13-KODE', 'categories': [{'measurements': [{'value': '0.89', 'spread': '0.6', 'groupId': 'OG000'}]}]}, {'title': '12,13-Ep-9-KODE', 'categories': [{'measurements': [{'value': '3.4', 'spread': '1.8', 'groupId': 'OG000'}]}]}, {'title': '5-KETE', 'categories': [{'measurements': [{'value': '0.02', 'spread': '0.017', 'groupId': 'OG000'}]}]}, {'title': 'LTB5', 'categories': [{'measurements': [{'value': '0.099', 'spread': '0.082', 'groupId': 'OG000'}]}]}, {'title': '10-Nitrooleate', 'categories': [{'measurements': [{'value': '0.074', 'spread': '0.043', 'groupId': 'OG000'}]}]}, {'title': '10-Nitrolinoleate', 'categories': [{'measurements': [{'value': '0.087', 'spread': '0.078', 'groupId': 'OG000'}]}]}, {'title': 'PGE1', 'categories': [{'measurements': [{'value': '0.85', 'spread': '1', 'groupId': 'OG000'}]}]}, {'title': 'PGE2', 'categories': [{'measurements': [{'value': '1.1', 'spread': '1.3', 'groupId': 'OG000'}]}]}, {'title': 'PGF2a', 'categories': [{'measurements': [{'value': '0.44', 'spread': '0.74', 'groupId': 'OG000'}]}]}, {'title': '15-deoxy PGJ2', 'categories': [{'measurements': [{'value': '0.046', 'spread': '0.036', 'groupId': 'OG000'}]}]}, {'title': 'PGE3', 'categories': [{'measurements': [{'value': '0.18', 'spread': '0.21', 'groupId': 'OG000'}]}]}, {'title': 'Sum TriHOMEs', 'categories': [{'measurements': [{'value': '130', 'spread': '130', 'groupId': 'OG000'}]}]}, {'title': 'PEA', 'categories': [{'measurements': [{'value': '14', 'spread': '25', 'groupId': 'OG000'}]}]}, {'title': 'SEA', 'categories': [{'measurements': [{'value': '15', 'spread': '25', 'groupId': 'OG000'}]}]}, {'title': 'OEA', 'categories': [{'measurements': [{'value': '0.11', 'spread': '0.093', 'groupId': 'OG000'}]}]}, {'title': 'LEA', 'categories': [{'measurements': [{'value': '0.019', 'spread': '0.01', 'groupId': 'OG000'}]}]}, {'title': 'aLEA', 'categories': [{'measurements': [{'value': '0.0033', 'spread': '0.0024', 'groupId': 'OG000'}]}]}, {'title': 'Dihomo GLA EA', 'categories': [{'measurements': [{'value': '0.0023', 'spread': '0.0036', 'groupId': 'OG000'}]}]}, {'title': 'AEA', 'categories': [{'measurements': [{'value': '0.017', 'spread': '0.025', 'groupId': 'OG000'}]}]}, {'title': 'DEA', 'categories': [{'measurements': [{'value': '0.015', 'spread': '0.013', 'groupId': 'OG000'}]}]}, {'title': '1-OG', 'categories': [{'measurements': [{'value': '11', 'spread': '14', 'groupId': 'OG000'}]}]}, {'title': '2-OG', 'categories': [{'measurements': [{'value': '7', 'spread': '7.3', 'groupId': 'OG000'}]}]}, {'title': '1-LG', 'categories': [{'measurements': [{'value': '2.1', 'spread': '1.8', 'groupId': 'OG000'}]}]}, {'title': '2-LG', 'categories': [{'measurements': [{'value': '0.34', 'spread': '0.36', 'groupId': 'OG000'}]}]}, {'title': '1-AG', 'categories': [{'measurements': [{'value': '1.8', 'spread': '3.1', 'groupId': 'OG000'}]}]}, {'title': '2-AG', 'categories': [{'measurements': [{'value': '0.79', 'spread': '1', 'groupId': 'OG000'}]}]}, {'title': 'NO-Gly', 'categories': [{'measurements': [{'value': '0.15', 'spread': '0.13', 'groupId': 'OG000'}]}]}, {'title': 'NA-Gly', 'categories': [{'measurements': [{'value': '0.01', 'spread': '0.012', 'groupId': 'OG000'}]}]}, {'title': 'C14 Ceramide', 'categories': [{'measurements': [{'value': '0.23', 'spread': '0.18', 'groupId': 'OG000'}]}]}, {'title': 'C16 Ceramide', 'categories': [{'measurements': [{'value': '1', 'spread': '1', 'groupId': 'OG000'}]}]}, {'title': 'C18 Ceramide', 'categories': [{'measurements': [{'value': '0.29', 'spread': '0.34', 'groupId': 'OG000'}]}]}, {'title': 'C20 Ceramide', 'categories': [{'measurements': [{'value': '0.36', 'spread': '0.34', 'groupId': 'OG000'}]}]}, {'title': 'C24 Ceramide', 'categories': [{'measurements': [{'value': '0.88', 'spread': '0.8', 'groupId': 'OG000'}]}]}, {'title': '18:1 Sphingosine', 'categories': [{'measurements': [{'value': '4.9', 'spread': '4.6', 'groupId': 'OG000'}]}]}], 'paramType': 'MEAN', 'timeFrame': 'measured at study visit 1, detected lipid mediator concentrations reported', 'description': 'Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids.', 'unitOfMeasure': 'picomoles per milliliter', 'dispersionType': 'Standard Deviation', 'reportingStatus': 'POSTED', 'populationDescription': 'Of the 14 subjects recruited for this study, only 7 provided usable data for this primary outcome.'}, {'type': 'PRIMARY', 'title': 'Lower Back Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation', 'denoms': [{'units': 'Participants', 'counts': [{'value': '4', 'groupId': 'OG000'}]}], 'groups': [{'id': 'OG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'classes': [{'title': '9-HODE', 'categories': [{'measurements': [{'value': '17', 'spread': '11', 'groupId': 'OG000'}]}]}, {'title': '13-HODE', 'categories': [{'measurements': [{'value': '81', 'spread': '42', 'groupId': 'OG000'}]}]}, {'title': '9-HOTrE', 'categories': [{'measurements': [{'value': '1.8', 'spread': '0.65', 'groupId': 'OG000'}]}]}, {'title': '13-HOTrE', 'categories': [{'measurements': [{'value': '2.6', 'spread': '1.1', 'groupId': 'OG000'}]}]}, {'title': '5-HETE', 'categories': [{'measurements': [{'value': '0.15', 'spread': '0.1', 'groupId': 'OG000'}]}]}, {'title': '8-HETE', 'categories': [{'measurements': [{'value': '0.23', 'spread': '0.21', 'groupId': 'OG000'}]}]}, {'title': '9-HETE', 'categories': [{'measurements': [{'value': '0.12', 'spread': '0.083', 'groupId': 'OG000'}]}]}, {'title': '11-HETE', 'categories': [{'measurements': [{'value': '0.2', 'spread': '0.27', 'groupId': 'OG000'}]}]}, {'title': '12-HETE', 'categories': [{'measurements': [{'value': '0.3', 'spread': '0.22', 'groupId': 'OG000'}]}]}, {'title': '15-HETE', 'categories': [{'measurements': [{'value': '0.32', 'spread': '0.2', 'groupId': 'OG000'}]}]}, {'title': '12-HEPE', 'categories': [{'measurements': [{'value': '0.15', 'spread': '0.11', 'groupId': 'OG000'}]}]}, {'title': '9,10-e-DiHO', 'categories': [{'measurements': [{'value': '36', 'spread': '13', 'groupId': 'OG000'}]}]}, {'title': '9,10-DiHOME', 'categories': [{'measurements': [{'value': '2.6', 'spread': '0.73', 'groupId': 'OG000'}]}]}, {'title': '12,13-DiHOME', 'categories': [{'measurements': [{'value': '2.2', 'spread': '0.52', 'groupId': 'OG000'}]}]}, {'title': '9,10-DiHODE', 'categories': [{'measurements': [{'value': '0.38', 'spread': '0.16', 'groupId': 'OG000'}]}]}, {'title': '12,13-DiHODE', 'categories': [{'measurements': [{'value': '0.34', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': '15,16-DiHODE', 'categories': [{'measurements': [{'value': '0.55', 'spread': '0.16', 'groupId': 'OG000'}]}]}, {'title': '5,6-DiHETrE', 'categories': [{'measurements': [{'value': '2.8', 'spread': '1.8', 'groupId': 'OG000'}]}]}, {'title': '8,9-DiHETrE', 'categories': [{'measurements': [{'value': '0.1', 'spread': '0.22', 'groupId': 'OG000'}]}]}, {'title': '11,12-DiHETrE', 'categories': [{'measurements': [{'value': '0.28', 'spread': '0.039', 'groupId': 'OG000'}]}]}, {'title': '14,15-DiHETrE', 'categories': [{'measurements': [{'value': '0.34', 'spread': '0.067', 'groupId': 'OG000'}]}]}, {'title': '9,10-EpOME', 'categories': [{'measurements': [{'value': '1.6', 'spread': '1.1', 'groupId': 'OG000'}]}]}, {'title': '12,13-EpOME', 'categories': [{'measurements': [{'value': '1.6', 'spread': '1.3', 'groupId': 'OG000'}]}]}, {'title': '9,10-EpODE', 'categories': [{'measurements': [{'value': '0.28', 'spread': '0.15', 'groupId': 'OG000'}]}]}, {'title': '12,13-EpODE', 'categories': [{'measurements': [{'value': '0.2', 'spread': '0.1', 'groupId': 'OG000'}]}]}, {'title': '15,16-EpODE', 'categories': [{'measurements': [{'value': '0.49', 'spread': '0.34', 'groupId': 'OG000'}]}]}, {'title': '9-KODE', 'categories': [{'measurements': [{'value': '11', 'spread': '9.2', 'groupId': 'OG000'}]}]}, {'title': '13-KODE', 'categories': [{'measurements': [{'value': '6', 'spread': '2.9', 'groupId': 'OG000'}]}]}, {'title': '12,13-Ep-9-KODE', 'categories': [{'measurements': [{'value': '2.6', 'spread': '0.94', 'groupId': 'OG000'}]}]}, {'title': '5-KETE', 'categories': [{'measurements': [{'value': '0.1', 'spread': '0.12', 'groupId': 'OG000'}]}]}, {'title': 'LTB5', 'categories': [{'measurements': [{'value': '0.13', 'spread': '0.093', 'groupId': 'OG000'}]}]}, {'title': '10-Nitrooleate', 'categories': [{'measurements': [{'value': '0.75', 'spread': '1.2', 'groupId': 'OG000'}]}]}, {'title': '10-Nitrolinoleate', 'categories': [{'measurements': [{'value': '0.26', 'spread': '0.31', 'groupId': 'OG000'}]}]}, {'title': 'PGE1', 'categories': [{'measurements': [{'value': '0.11', 'spread': '0.046', 'groupId': 'OG000'}]}]}, {'title': 'PGE2', 'categories': [{'measurements': [{'value': '0.62', 'spread': '0.28', 'groupId': 'OG000'}]}]}, {'title': 'PGF2a', 'categories': [{'measurements': [{'value': '0.27', 'spread': '0.15', 'groupId': 'OG000'}]}]}, {'title': 'PGE3', 'categories': [{'measurements': [{'value': '0.17', 'spread': '0.13', 'groupId': 'OG000'}]}]}, {'title': 'Sum TriHOMEs', 'categories': [{'measurements': [{'value': '12', 'spread': '11', 'groupId': 'OG000'}]}]}, {'title': 'PEA', 'categories': [{'measurements': [{'value': '47', 'spread': '65', 'groupId': 'OG000'}]}]}, {'title': 'SEA', 'categories': [{'measurements': [{'value': '53', 'spread': '73', 'groupId': 'OG000'}]}]}, {'title': 'OEA', 'categories': [{'measurements': [{'value': '0.55', 'spread': '0.46', 'groupId': 'OG000'}]}]}, {'title': 'LEA', 'categories': [{'measurements': [{'value': '0.14', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': 'aLEA', 'categories': [{'measurements': [{'value': '0.11', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': 'Dihomo GLA EA', 'categories': [{'measurements': [{'value': '0.05', 'spread': '0.083', 'groupId': 'OG000'}]}]}, {'title': 'AEA', 'categories': [{'measurements': [{'value': '0.14', 'spread': '0.19', 'groupId': 'OG000'}]}]}, {'title': 'DEA', 'categories': [{'measurements': [{'value': '0.028', 'spread': '0.069', 'groupId': 'OG000'}]}]}, {'title': '1-OG', 'categories': [{'measurements': [{'value': '300', 'spread': '210', 'groupId': 'OG000'}]}]}, {'title': '2-OG', 'categories': [{'measurements': [{'value': '46', 'spread': '47', 'groupId': 'OG000'}]}]}, {'title': '1-LG', 'categories': [{'measurements': [{'value': '110', 'spread': '82', 'groupId': 'OG000'}]}]}, {'title': '2-LG', 'categories': [{'measurements': [{'value': '2.6', 'spread': '1.3', 'groupId': 'OG000'}]}]}, {'title': '1-AG', 'categories': [{'measurements': [{'value': '10', 'spread': '12', 'groupId': 'OG000'}]}]}, {'title': '2-AG', 'categories': [{'measurements': [{'value': '2.6', 'spread': '1.3', 'groupId': 'OG000'}]}]}, {'title': 'NO-Gly', 'categories': [{'measurements': [{'value': '0.25', 'spread': '0.26', 'groupId': 'OG000'}]}]}, {'title': 'NA-Gly', 'categories': [{'measurements': [{'value': '0.027', 'spread': '0.057', 'groupId': 'OG000'}]}]}, {'title': 'C14 Ceramide', 'categories': [{'measurements': [{'value': '0.49', 'spread': '0.28', 'groupId': 'OG000'}]}]}, {'title': 'C16 Ceramide', 'categories': [{'measurements': [{'value': '1.7', 'spread': '1.6', 'groupId': 'OG000'}]}]}, {'title': 'C18 Ceramide', 'categories': [{'measurements': [{'value': '0.34', 'spread': '0.32', 'groupId': 'OG000'}]}]}, {'title': 'C20 Ceramide', 'categories': [{'measurements': [{'value': '0.33', 'spread': '0.26', 'groupId': 'OG000'}]}]}, {'title': 'C24 Ceramide', 'categories': [{'measurements': [{'value': '0.41', 'spread': '0.28', 'groupId': 'OG000'}]}]}, {'title': '18:1 Sphingosine', 'categories': [{'measurements': [{'value': '8.1', 'spread': '12', 'groupId': 'OG000'}]}]}], 'paramType': 'MEAN', 'timeFrame': 'measured at study visit 1, detected lipid mediator concentrations reported', 'description': 'Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids.', 'unitOfMeasure': 'picomoles per milliliter', 'dispersionType': 'Standard Deviation', 'reportingStatus': 'POSTED', 'populationDescription': 'Of the 14 subjects recruited for this study, only 4 provided usable data for this primary outcome.'}, {'type': 'PRIMARY', 'title': 'Anterior Distal Thigh Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation', 'denoms': [{'units': 'Participants', 'counts': [{'value': '4', 'groupId': 'OG000'}]}], 'groups': [{'id': 'OG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'classes': [{'title': '9-HODE', 'categories': [{'measurements': [{'value': '19', 'spread': '9.9', 'groupId': 'OG000'}]}]}, {'title': '13-HODE', 'categories': [{'measurements': [{'value': '48', 'spread': '24', 'groupId': 'OG000'}]}]}, {'title': '9-HOTrE', 'categories': [{'measurements': [{'value': '1.8', 'spread': '0.54', 'groupId': 'OG000'}]}]}, {'title': '13-HOTrE', 'categories': [{'measurements': [{'value': '5.2', 'spread': '1', 'groupId': 'OG000'}]}]}, {'title': '5-HETE', 'categories': [{'measurements': [{'value': '0.067', 'spread': '0.059', 'groupId': 'OG000'}]}]}, {'title': '8-HETE', 'categories': [{'measurements': [{'value': '0.17', 'spread': '0.066', 'groupId': 'OG000'}]}]}, {'title': '9-HETE', 'categories': [{'measurements': [{'value': '0.12', 'spread': '0.043', 'groupId': 'OG000'}]}]}, {'title': '11-HETE', 'categories': [{'measurements': [{'value': '0.12', 'spread': '0.075', 'groupId': 'OG000'}]}]}, {'title': '12-HETE', 'categories': [{'measurements': [{'value': '0.31', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': '15-HETE', 'categories': [{'measurements': [{'value': '0.26', 'spread': '0.28', 'groupId': 'OG000'}]}]}, {'title': '12-HEPE', 'categories': [{'measurements': [{'value': '0.13', 'spread': '0.057', 'groupId': 'OG000'}]}]}, {'title': '9,10-e-DiHO', 'categories': [{'measurements': [{'value': '35', 'spread': '5.5', 'groupId': 'OG000'}]}]}, {'title': '9,10-DiHOME', 'categories': [{'measurements': [{'value': '2.9', 'spread': '0.89', 'groupId': 'OG000'}]}]}, {'title': '12,13-DiHOME', 'categories': [{'measurements': [{'value': '2.4', 'spread': '0.69', 'groupId': 'OG000'}]}]}, {'title': '9,10-DiHODE', 'categories': [{'measurements': [{'value': '0.46', 'spread': '0.095', 'groupId': 'OG000'}]}]}, {'title': '12,13-DiHODE', 'categories': [{'measurements': [{'value': '0.7', 'spread': '0.13', 'groupId': 'OG000'}]}]}, {'title': '15,16-DiHODE', 'categories': [{'measurements': [{'value': '0.99', 'spread': '0.31', 'groupId': 'OG000'}]}]}, {'title': '5,6-DiHETrE', 'categories': [{'measurements': [{'value': '2.7', 'spread': '1.2', 'groupId': 'OG000'}]}]}, {'title': '8,9-DiHETrE', 'categories': [{'measurements': [{'value': '0.62', 'spread': '0.45', 'groupId': 'OG000'}]}]}, {'title': '11,12-DiHETrE', 'categories': [{'measurements': [{'value': '0.19', 'spread': '0.072', 'groupId': 'OG000'}]}]}, {'title': '14,15-DiHETrE', 'categories': [{'measurements': [{'value': '0.19', 'spread': '0.087', 'groupId': 'OG000'}]}]}, {'title': '9,10-EpOME', 'categories': [{'measurements': [{'value': '1.7', 'spread': '0.54', 'groupId': 'OG000'}]}]}, {'title': '12,13-EpOME', 'categories': [{'measurements': [{'value': '1.6', 'spread': '0.47', 'groupId': 'OG000'}]}]}, {'title': '9,10-EpODE', 'categories': [{'measurements': [{'value': '0.8', 'spread': '0.66', 'groupId': 'OG000'}]}]}, {'title': '12,13-EpODE', 'categories': [{'measurements': [{'value': '0.25', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': '15,16-EpODE', 'categories': [{'measurements': [{'value': '0.95', 'spread': '0.45', 'groupId': 'OG000'}]}]}, {'title': '9-KODE', 'categories': [{'measurements': [{'value': '21', 'spread': '17', 'groupId': 'OG000'}]}]}, {'title': '13-KODE', 'categories': [{'measurements': [{'value': '9.3', 'spread': '7.3', 'groupId': 'OG000'}]}]}, {'title': '12,13-Ep-9-KODE', 'categories': [{'measurements': [{'value': '3.3', 'spread': '1.4', 'groupId': 'OG000'}]}]}, {'title': '5-KETE', 'categories': [{'measurements': [{'value': '0.04', 'spread': '0.018', 'groupId': 'OG000'}]}]}, {'title': 'LTB5', 'categories': [{'measurements': [{'value': '0.088', 'spread': '0.068', 'groupId': 'OG000'}]}]}, {'title': '10-Nitrooleate', 'categories': [{'measurements': [{'value': '0.12', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': '10-Nitrolinoleate', 'categories': [{'measurements': [{'value': '0.16', 'spread': '0.019', 'groupId': 'OG000'}]}]}, {'title': 'PGE1', 'categories': [{'measurements': [{'value': '0.18', 'spread': '0.15', 'groupId': 'OG000'}]}]}, {'title': 'PGE2', 'categories': [{'measurements': [{'value': '0.36', 'spread': '0.38', 'groupId': 'OG000'}]}]}, {'title': 'PGF2a', 'categories': [{'measurements': [{'value': '0.44', 'spread': '0.45', 'groupId': 'OG000'}]}]}, {'title': 'PGE3', 'categories': [{'measurements': [{'value': '0.15', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': 'Sum TriHOMEs', 'categories': [{'measurements': [{'value': '36', 'spread': '14', 'groupId': 'OG000'}]}]}, {'title': 'PEA', 'categories': [{'measurements': [{'value': '18', 'spread': '6.4', 'groupId': 'OG000'}]}]}, {'title': 'SEA', 'categories': [{'measurements': [{'value': '18', 'spread': '5.8', 'groupId': 'OG000'}]}]}, {'title': 'OEA', 'categories': [{'measurements': [{'value': '0.31', 'spread': '0.24', 'groupId': 'OG000'}]}]}, {'title': 'LEA', 'categories': [{'measurements': [{'value': '0.078', 'spread': '0.039', 'groupId': 'OG000'}]}]}, {'title': 'aLEA', 'categories': [{'measurements': [{'value': '0.022', 'spread': '0.005', 'groupId': 'OG000'}]}]}, {'title': 'Dihomo GLA EA', 'categories': [{'measurements': [{'value': '0.00037', 'spread': '0.0058', 'groupId': 'OG000'}]}]}, {'title': 'AEA', 'categories': [{'measurements': [{'value': '0.013', 'spread': '0.005', 'groupId': 'OG000'}]}]}, {'title': 'DEA', 'categories': [{'measurements': [{'value': '0.053', 'spread': '0.061', 'groupId': 'OG000'}]}]}, {'title': '1-OG', 'categories': [{'measurements': [{'value': '200', 'spread': '88', 'groupId': 'OG000'}]}]}, {'title': '2-OG', 'categories': [{'measurements': [{'value': '37', 'spread': '16', 'groupId': 'OG000'}]}]}, {'title': '1-LG', 'categories': [{'measurements': [{'value': '81', 'spread': '41', 'groupId': 'OG000'}]}]}, {'title': '2-LG', 'categories': [{'measurements': [{'value': '2.1', 'spread': '0.6', 'groupId': 'OG000'}]}]}, {'title': '1-AG', 'categories': [{'measurements': [{'value': '13', 'spread': '5.4', 'groupId': 'OG000'}]}]}, {'title': '2-AG', 'categories': [{'measurements': [{'value': '4.2', 'spread': '0.72', 'groupId': 'OG000'}]}]}, {'title': 'NO-Gly', 'categories': [{'measurements': [{'value': '0.23', 'spread': '0.14', 'groupId': 'OG000'}]}]}, {'title': 'NA-Gly', 'categories': [{'measurements': [{'value': '0.02', 'spread': '0.013', 'groupId': 'OG000'}]}]}, {'title': 'C14 Ceramide', 'categories': [{'measurements': [{'value': '0.24', 'spread': '0.15', 'groupId': 'OG000'}]}]}, {'title': 'C16 Ceramide', 'categories': [{'measurements': [{'value': '1.5', 'spread': '1.2', 'groupId': 'OG000'}]}]}, {'title': 'C18 Ceramide', 'categories': [{'measurements': [{'value': '0.31', 'spread': '0.25', 'groupId': 'OG000'}]}]}, {'title': 'C20 Ceramide', 'categories': [{'measurements': [{'value': '0.36', 'spread': '0.32', 'groupId': 'OG000'}]}]}, {'title': 'C24 Ceramide', 'categories': [{'measurements': [{'value': '1', 'spread': '0.99', 'groupId': 'OG000'}]}]}, {'title': '18:1 Sphingosine', 'categories': [{'measurements': [{'value': '2.2', 'spread': '4.5', 'groupId': 'OG000'}]}]}], 'paramType': 'MEAN', 'timeFrame': 'measured at study visit 2, detected lipid mediator concentrations reported', 'description': 'Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids.', 'unitOfMeasure': 'picomoles per milliliter', 'dispersionType': 'Standard Deviation', 'reportingStatus': 'POSTED', 'populationDescription': 'Of the 14 subjects recruited for this study, only 4 provided usable data for this primary outcome.'}, {'type': 'PRIMARY', 'title': 'Change in Plasma Lipid Mediator Concentrations Before and After Oral Ibuprofen Administration', 'denoms': [{'units': 'Participants', 'counts': [{'value': '9', 'groupId': 'OG000'}]}], 'groups': [{'id': 'OG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'classes': [{'title': 'PGE1', 'categories': [{'measurements': [{'value': '0.126', 'groupId': 'OG000', 'lowerLimit': '0.04', 'upperLimit': '0.421'}]}]}, {'title': 'PGE2', 'categories': [{'measurements': [{'value': '0.159', 'groupId': 'OG000', 'lowerLimit': '0.06', 'upperLimit': '0.6'}]}]}, {'title': 'PGF2a', 'categories': [{'measurements': [{'value': '5.47', 'groupId': 'OG000', 'lowerLimit': '1.43', 'upperLimit': '23.7'}]}]}, {'title': '13-HODE', 'categories': [{'measurements': [{'value': '23', 'groupId': 'OG000', 'lowerLimit': '11.7', 'upperLimit': '40.7'}]}]}, {'title': '9-HODE', 'categories': [{'measurements': [{'value': '8.77', 'groupId': 'OG000', 'lowerLimit': '5.38', 'upperLimit': '16'}]}]}, {'title': '13-HOTE', 'categories': [{'measurements': [{'value': '1.56', 'groupId': 'OG000', 'lowerLimit': '0.92', 'upperLimit': '4.65'}]}]}, {'title': '9-HOTE', 'categories': [{'measurements': [{'value': '1.19', 'groupId': 'OG000', 'lowerLimit': '0.71', 'upperLimit': '2.43'}]}]}, {'title': '15-HETE', 'categories': [{'measurements': [{'value': '0.861', 'groupId': 'OG000', 'lowerLimit': '0.14', 'upperLimit': '3.56'}]}]}, {'title': '12-HETE', 'categories': [{'measurements': [{'value': '1.31', 'groupId': 'OG000', 'lowerLimit': '0.66', 'upperLimit': '6.38'}]}]}, {'title': '11-HETE', 'categories': [{'measurements': [{'value': '0.408', 'groupId': 'OG000', 'lowerLimit': '0.2', 'upperLimit': '0.88'}]}]}, {'title': '5-HETE', 'categories': [{'measurements': [{'value': '0.425', 'groupId': 'OG000', 'lowerLimit': '0.02', 'upperLimit': '1.86'}]}]}, {'title': '17-HDoHE', 'categories': [{'measurements': [{'value': '2.55', 'groupId': 'OG000', 'lowerLimit': '0.1', 'upperLimit': '34.7'}]}]}, {'title': '9,10-e-DiHO', 'categories': [{'measurements': [{'value': '10.4', 'groupId': 'OG000', 'lowerLimit': '5.76', 'upperLimit': '34.1'}]}]}, {'title': '12,13-DiHOME', 'categories': [{'measurements': [{'value': '4.13', 'groupId': 'OG000', 'lowerLimit': '2.24', 'upperLimit': '10.4'}]}]}, {'title': '9,10-DiHOME', 'categories': [{'measurements': [{'value': '4.44', 'groupId': 'OG000', 'lowerLimit': '1.44', 'upperLimit': '12'}]}]}, {'title': '15,16-DiHODE', 'categories': [{'measurements': [{'value': '15', 'groupId': 'OG000', 'lowerLimit': '6.96', 'upperLimit': '37.5'}]}]}, {'title': '12,13-DiHODE', 'categories': [{'measurements': [{'value': '1.77', 'groupId': 'OG000', 'lowerLimit': '0.3', 'upperLimit': '7.52'}]}]}, {'title': '9,10-DiHODE', 'categories': [{'measurements': [{'value': '0.458', 'groupId': 'OG000', 'lowerLimit': '0.2', 'upperLimit': '1.04'}]}]}, {'title': '14,15-DiHETrE', 'categories': [{'measurements': [{'value': '1.17', 'groupId': 'OG000', 'lowerLimit': '0.7', 'upperLimit': '2.77'}]}]}, {'title': '11,12-DiHETrE', 'categories': [{'measurements': [{'value': '1.11', 'groupId': 'OG000', 'lowerLimit': '0.56', 'upperLimit': '3.09'}]}]}, {'title': '5,6-DiHETrE', 'categories': [{'measurements': [{'value': '0.406', 'groupId': 'OG000', 'lowerLimit': '0.06', 'upperLimit': '1.04'}]}]}, {'title': '17,18-DiHETE', 'categories': [{'measurements': [{'value': '20.2', 'groupId': 'OG000', 'lowerLimit': '7.4', 'upperLimit': '33.2'}]}]}, {'title': '19,20-DiHDoPA', 'categories': [{'measurements': [{'value': '1.91', 'groupId': 'OG000', 'lowerLimit': '0.76', 'upperLimit': '4.13'}]}]}, {'title': 'Sum TriHOMEs', 'categories': [{'measurements': [{'value': '7.88', 'groupId': 'OG000', 'lowerLimit': '4.64', 'upperLimit': '12.2'}]}]}, {'title': '9(10)-EpO', 'categories': [{'measurements': [{'value': '9.96', 'groupId': 'OG000', 'lowerLimit': '2.37', 'upperLimit': '45.8'}]}]}, {'title': '12(13)-EpOME', 'categories': [{'measurements': [{'value': '4.1', 'groupId': 'OG000', 'lowerLimit': '1.14', 'upperLimit': '11.8'}]}]}, {'title': '9(10)-EpOME', 'categories': [{'measurements': [{'value': '1.12', 'groupId': 'OG000', 'lowerLimit': '0.26', 'upperLimit': '4.7'}]}]}, {'title': '15(16)-EpODE', 'categories': [{'measurements': [{'value': '3.95', 'groupId': 'OG000', 'lowerLimit': '0.95', 'upperLimit': '11.3'}]}]}, {'title': '9(10)-EpODE', 'categories': [{'measurements': [{'value': '1.02', 'groupId': 'OG000', 'lowerLimit': '0.35', 'upperLimit': '10.2'}]}]}, {'title': '13-KODE', 'categories': [{'measurements': [{'value': '1.89', 'groupId': 'OG000', 'lowerLimit': '0.13', 'upperLimit': '19.3'}]}]}, {'title': '9-KODE', 'categories': [{'measurements': [{'value': '3.09', 'groupId': 'OG000', 'lowerLimit': '0.67', 'upperLimit': '15.7'}]}]}, {'title': 'OEA', 'categories': [{'measurements': [{'value': '4.14', 'groupId': 'OG000', 'lowerLimit': '2.83', 'upperLimit': '8.65'}]}]}, {'title': 'LEA', 'categories': [{'measurements': [{'value': '1.19', 'groupId': 'OG000', 'lowerLimit': '0.67', 'upperLimit': '1.95'}]}]}, {'title': 'aLEA', 'categories': [{'measurements': [{'value': '0.0968', 'groupId': 'OG000', 'lowerLimit': '0.04', 'upperLimit': '0.29'}]}]}, {'title': 'Dihomo GLA EA', 'categories': [{'measurements': [{'value': '0.168', 'groupId': 'OG000', 'lowerLimit': '0.05', 'upperLimit': '0.39'}]}]}, {'title': 'AEA', 'categories': [{'measurements': [{'value': '0.627', 'groupId': 'OG000', 'lowerLimit': '0.29', 'upperLimit': '0.92'}]}]}, {'title': 'DEA', 'categories': [{'measurements': [{'value': '0.298', 'groupId': 'OG000', 'lowerLimit': '0.21', 'upperLimit': '0.48'}]}]}, {'title': 'DHEA', 'categories': [{'measurements': [{'value': '0.76', 'groupId': 'OG000', 'lowerLimit': '0.28', 'upperLimit': '1.46'}]}]}, {'title': 'NO-Gly', 'categories': [{'measurements': [{'value': '3.5', 'groupId': 'OG000', 'lowerLimit': '1.62', 'upperLimit': '9.44'}]}]}, {'title': 'NA-Gly', 'categories': [{'measurements': [{'value': '0.833', 'groupId': 'OG000', 'lowerLimit': '0.34', 'upperLimit': '1.63'}]}]}, {'title': 'OG', 'categories': [{'measurements': [{'value': '393', 'groupId': 'OG000', 'lowerLimit': '212', 'upperLimit': '702'}]}]}, {'title': 'LG', 'categories': [{'measurements': [{'value': '238', 'groupId': 'OG000', 'lowerLimit': '67.6', 'upperLimit': '820'}]}]}, {'title': 'AG', 'categories': [{'measurements': [{'value': '20.7', 'groupId': 'OG000', 'lowerLimit': '10', 'upperLimit': '51.2'}]}]}], 'paramType': 'GEOMETRIC_MEAN', 'timeFrame': 'measured at four timepoints at study visit 4, detected lipid mediator concentrations for first timepoint reported', 'description': 'Approximately 100 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids, and endocannabinoid-like molecules.', 'unitOfMeasure': 'picomoles per milliliter', 'dispersionType': 'Full Range', 'reportingStatus': 'POSTED', 'populationDescription': 'Of the 14 subjects recruited for this study, only 9 provided usable data for this primary outcome.'}, {'type': 'SECONDARY', 'title': 'Change in Pilocarpine-stimulated Sweat Lipid Mediator Concentrations Before and After Oral Ibuprofen Administration', 'denoms': [{'units': 'Participants', 'counts': [{'value': '9', 'groupId': 'OG000'}]}], 'groups': [{'id': 'OG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'classes': [{'title': 'PGE1', 'categories': [{'measurements': [{'value': '0.453', 'groupId': 'OG000', 'lowerLimit': '0.12', 'upperLimit': '4.91'}]}]}, {'title': 'PGE2', 'categories': [{'measurements': [{'value': '2.71', 'groupId': 'OG000', 'lowerLimit': '0.34', 'upperLimit': '51.6'}]}]}, {'title': '13-HODE', 'categories': [{'measurements': [{'value': '21.9', 'groupId': 'OG000', 'lowerLimit': '3.72', 'upperLimit': '69.6'}]}]}, {'title': '9-HODE', 'categories': [{'measurements': [{'value': '7.72', 'groupId': 'OG000', 'lowerLimit': '1.69', 'upperLimit': '23.4'}]}]}, {'title': '13-HOTE', 'categories': [{'measurements': [{'value': '1.08', 'groupId': 'OG000', 'lowerLimit': '0.29', 'upperLimit': '3.23'}]}]}, {'title': '9-HOTE', 'categories': [{'measurements': [{'value': '1.08', 'groupId': 'OG000', 'lowerLimit': '0.53', 'upperLimit': '3.25'}]}]}, {'title': '20-HETE', 'categories': [{'measurements': [{'value': '0.742', 'groupId': 'OG000', 'lowerLimit': '0.25', 'upperLimit': '1.74'}]}]}, {'title': '15-HETE', 'categories': [{'measurements': [{'value': '0.311', 'groupId': 'OG000', 'lowerLimit': '0.06', 'upperLimit': '4.05'}]}]}, {'title': '12-HETE', 'categories': [{'measurements': [{'value': '0.965', 'groupId': 'OG000', 'lowerLimit': '0.52', 'upperLimit': '1.7'}]}]}, {'title': '11-HETE', 'categories': [{'measurements': [{'value': '0.0882', 'groupId': 'OG000', 'lowerLimit': '0.01', 'upperLimit': '0.39'}]}]}, {'title': '8-HETE', 'categories': [{'measurements': [{'value': '0.0618', 'groupId': 'OG000', 'lowerLimit': '0.01', 'upperLimit': '0.18'}]}]}, {'title': '5-HETE', 'categories': [{'measurements': [{'value': '0.138', 'groupId': 'OG000', 'lowerLimit': '0.04', 'upperLimit': '0.36'}]}]}, {'title': '15-HEPE', 'categories': [{'measurements': [{'value': '0.0576', 'groupId': 'OG000', 'lowerLimit': '0.00136', 'upperLimit': '0.3'}]}]}, {'title': '12-HEPE', 'categories': [{'measurements': [{'value': '0.0781', 'groupId': 'OG000', 'lowerLimit': '0.01', 'upperLimit': '0.23'}]}]}, {'title': '17-HDoHE', 'categories': [{'measurements': [{'value': '0.46', 'groupId': 'OG000', 'lowerLimit': '0.09', 'upperLimit': '7.41'}]}]}, {'title': '9,10-e-DiHO', 'categories': [{'measurements': [{'value': '58.4', 'groupId': 'OG000', 'lowerLimit': '39', 'upperLimit': '135'}]}]}, {'title': '12,13-DiHOME', 'categories': [{'measurements': [{'value': '1.89', 'groupId': 'OG000', 'lowerLimit': '0.9', 'upperLimit': '3.6'}]}]}, {'title': '9,10-DiHOME', 'categories': [{'measurements': [{'value': '3.61', 'groupId': 'OG000', 'lowerLimit': '1.85', 'upperLimit': '7.66'}]}]}, {'title': '15,16-DiHODE', 'categories': [{'measurements': [{'value': '1.49', 'groupId': 'OG000', 'lowerLimit': '0.43', 'upperLimit': '5.65'}]}]}, {'title': '12,13-DiHODE', 'categories': [{'measurements': [{'value': '0.332', 'groupId': 'OG000', 'lowerLimit': '0.12', 'upperLimit': '1.34'}]}]}, {'title': '9,10-DiHODE', 'categories': [{'measurements': [{'value': '0.35', 'groupId': 'OG000', 'lowerLimit': '0.1', 'upperLimit': '0.87'}]}]}, {'title': '14,15-DiHETrE', 'categories': [{'measurements': [{'value': '0.341', 'groupId': 'OG000', 'lowerLimit': '0.09', 'upperLimit': '1.09'}]}]}, {'title': '11,12-DiHETrE', 'categories': [{'measurements': [{'value': '0.231', 'groupId': 'OG000', 'lowerLimit': '0.07', 'upperLimit': '1.02'}]}]}, {'title': '5,6-DiHETrE', 'categories': [{'measurements': [{'value': '3.29', 'groupId': 'OG000', 'lowerLimit': '1.28', 'upperLimit': '10.7'}]}]}, {'title': '17,18-DiHETE', 'categories': [{'measurements': [{'value': '1.76', 'groupId': 'OG000', 'lowerLimit': '0.31', 'upperLimit': '6.72'}]}]}, {'title': 'Sum TriHOMEs', 'categories': [{'measurements': [{'value': '31.7', 'groupId': 'OG000', 'lowerLimit': '11.4', 'upperLimit': '93.5'}]}]}, {'title': '9(10)-EpO', 'categories': [{'measurements': [{'value': '2.81', 'groupId': 'OG000', 'lowerLimit': '0.89', 'upperLimit': '6.54'}]}]}, {'title': '12(13)-EpOME', 'categories': [{'measurements': [{'value': '0.485', 'groupId': 'OG000', 'lowerLimit': '0.08', 'upperLimit': '1.31'}]}]}, {'title': '9(10)-EpOME', 'categories': [{'measurements': [{'value': '0.657', 'groupId': 'OG000', 'lowerLimit': '0.16', 'upperLimit': '1.93'}]}]}, {'title': '15(16)-EpODE', 'categories': [{'measurements': [{'value': '0.491', 'groupId': 'OG000', 'lowerLimit': '0.04', 'upperLimit': '4.5'}]}]}, {'title': '12(13)-EpODE', 'categories': [{'measurements': [{'value': '0.0703', 'groupId': 'OG000', 'lowerLimit': '0.02', 'upperLimit': '0.14'}]}]}, {'title': '9(10)-EpODE', 'categories': [{'measurements': [{'value': '0.194', 'groupId': 'OG000', 'lowerLimit': '0.04', 'upperLimit': '0.51'}]}]}, {'title': '13-KODE', 'categories': [{'measurements': [{'value': '2.17', 'groupId': 'OG000', 'lowerLimit': '0.3', 'upperLimit': '13.2'}]}]}, {'title': '9-KODE', 'categories': [{'measurements': [{'value': '5.35', 'groupId': 'OG000', 'lowerLimit': '0.64', 'upperLimit': '38.5'}]}]}, {'title': '12(13)-Ep-9-KODE', 'categories': [{'measurements': [{'value': '1.82', 'groupId': 'OG000', 'lowerLimit': '0.72', 'upperLimit': '3.64'}]}]}, {'title': '10-Nitrooleate', 'categories': [{'measurements': [{'value': '1.78', 'groupId': 'OG000', 'lowerLimit': '0.36', 'upperLimit': '6.24'}]}]}, {'title': '9-Nitrooleate', 'categories': [{'measurements': [{'value': '0.729', 'groupId': 'OG000', 'lowerLimit': '0.09', 'upperLimit': '3.76'}]}]}, {'title': '10-Nitrolinoleate', 'categories': [{'measurements': [{'value': '0.297', 'groupId': 'OG000', 'lowerLimit': '0.16', 'upperLimit': '0.6'}]}]}, {'title': 'OEA', 'categories': [{'measurements': [{'value': '0.586', 'groupId': 'OG000', 'lowerLimit': '0.09', 'upperLimit': '2.7'}]}]}, {'title': 'LEA', 'categories': [{'measurements': [{'value': '0.0447', 'groupId': 'OG000', 'lowerLimit': '0.01', 'upperLimit': '0.22'}]}]}, {'title': 'NO-Gly', 'categories': [{'measurements': [{'value': '0.156', 'groupId': 'OG000', 'lowerLimit': '0.01', 'upperLimit': '0.55'}]}]}, {'title': 'NA-Gly', 'categories': [{'measurements': [{'value': '0.0627', 'groupId': 'OG000', 'lowerLimit': '0.01', 'upperLimit': '0.23'}]}]}, {'title': 'OG', 'categories': [{'measurements': [{'value': '61.5', 'groupId': 'OG000', 'lowerLimit': '2.67', 'upperLimit': '5620'}]}]}, {'title': 'LG', 'categories': [{'measurements': [{'value': '4.11', 'groupId': 'OG000', 'lowerLimit': '0.46', 'upperLimit': '21.2'}]}]}, {'title': 'AG', 'categories': [{'measurements': [{'value': '4.2', 'groupId': 'OG000', 'lowerLimit': '0.15', 'upperLimit': '836'}]}]}], 'paramType': 'GEOMETRIC_MEAN', 'timeFrame': 'measured at four timepoints at study visit 4, detected lipid mediator concentrations for first timepoint reported', 'description': 'Approximately 100 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids, and endocannabinoid-like molecules.', 'unitOfMeasure': 'picomoles per milliliter', 'dispersionType': 'Full Range', 'reportingStatus': 'POSTED', 'populationDescription': 'Of the 14 subjects recruited for this study, only 9 provided usable data for this primary outcome.'}, {'type': 'SECONDARY', 'title': 'Change in Plasma Ibuprofen Concentrations', 'denoms': [{'units': 'Participants', 'counts': [{'value': '9', 'groupId': 'OG000'}]}], 'groups': [{'id': 'OG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'classes': [{'title': 'Prior to administration', 'categories': [{'measurements': [{'value': '0', 'groupId': 'OG000', 'lowerLimit': '0', 'upperLimit': '0'}]}]}, {'title': '30 min after administration', 'categories': [{'measurements': [{'value': '18.8', 'groupId': 'OG000', 'lowerLimit': '3.4', 'upperLimit': '65.3'}]}]}, {'title': '2 hr after administration', 'categories': [{'measurements': [{'value': '48.3', 'groupId': 'OG000', 'lowerLimit': '39.1', 'upperLimit': '75.9'}]}]}, {'title': '4 hr after administration', 'categories': [{'measurements': [{'value': '33.8', 'groupId': 'OG000', 'lowerLimit': '14.9', 'upperLimit': '90.1'}]}]}], 'paramType': 'GEOMETRIC_MEAN', 'timeFrame': 'measured at four timepoints at study visit 4', 'description': 'Plasma concentration of ibuprofen prior to and 30 min, 2 hr and 4 hr after oral administration', 'unitOfMeasure': 'micrograms per milliliter', 'dispersionType': 'Full Range', 'reportingStatus': 'POSTED', 'populationDescription': 'Of the 14 subjects recruited for this study, only 9 provided usable data for this primary outcome.'}, {'type': 'SECONDARY', 'title': 'Change in Sweat Ibuprofen Concentrations', 'denoms': [{'units': 'Participants', 'counts': [{'value': '9', 'groupId': 'OG000'}]}], 'groups': [{'id': 'OG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit COX metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'classes': [{'title': 'Prior to administration', 'categories': [{'measurements': [{'value': '0', 'groupId': 'OG000', 'lowerLimit': '0', 'upperLimit': '0'}]}]}, {'title': '30 min after administration', 'categories': [{'measurements': [{'value': '0', 'groupId': 'OG000', 'lowerLimit': '0', 'upperLimit': '0'}]}]}, {'title': '2 hr after administration', 'categories': [{'measurements': [{'value': '0', 'groupId': 'OG000', 'lowerLimit': '0', 'upperLimit': '0'}]}]}, {'title': '4 hr after administration', 'categories': [{'measurements': [{'value': '0', 'groupId': 'OG000', 'lowerLimit': '0', 'upperLimit': '0'}]}]}], 'paramType': 'GEOMETRIC_MEAN', 'timeFrame': 'measured at four timepoints at study visit 4', 'description': 'Sweat concentration of ibuprofen prior to and 30 min, 2 hr and 4 hr after oral administration', 'unitOfMeasure': 'micrograms per milliliter', 'dispersionType': 'Full Range', 'reportingStatus': 'POSTED', 'populationDescription': 'Of the 14 subjects recruited for this study, only 9 provided usable data for this primary outcome.'}]}, 'participantFlowModule': {'groups': [{'id': 'FG000', 'title': 'Single Group', 'description': 'Subjects will participate in 4 study visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit cyclooxygenase metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine'}], 'periods': [{'title': 'Overall Study', 'milestones': [{'type': 'STARTED', 'achievements': [{'groupId': 'FG000', 'numSubjects': '14'}]}, {'type': 'COMPLETED', 'achievements': [{'groupId': 'FG000', 'numSubjects': '13'}]}, {'type': 'NOT COMPLETED', 'achievements': [{'groupId': 'FG000', 'numSubjects': '1'}]}], 'dropWithdraws': [{'type': 'Subject dismissed', 'reasons': [{'groupId': 'FG000', 'numSubjects': '1'}]}]}]}, 'baselineCharacteristicsModule': {'denoms': [{'units': 'Participants', 'counts': [{'value': '14', 'groupId': 'BG000'}]}], 'groups': [{'id': 'BG000', 'title': 'Single Group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise on a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.\n\nIbuprofen: 400 mg ibuprofen given to inhibit cyclooxygenase (COX) metabolism\n\nPhysiological induction of sweating: 15 minutes exercise at 60-80% oxygen consumption to induce sweating\n\nPilocarpine: Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a'}], 'measures': [{'title': 'Age, Categorical', 'classes': [{'denoms': [{'units': 'Participants', 'counts': [{'value': '14', 'groupId': 'BG000'}]}], 'categories': [{'title': '<=18 years', 'measurements': [{'value': '0', 'groupId': 'BG000'}]}, {'title': 'Between 18 and 65 years', 'measurements': [{'value': '14', 'groupId': 'BG000'}]}, {'title': '>=65 years', 'measurements': [{'value': '0', 'groupId': 'BG000'}]}]}], 'paramType': 'COUNT_OF_PARTICIPANTS', 'unitOfMeasure': 'Participants'}, {'title': 'Sex: Female, Male', 'classes': [{'denoms': [{'units': 'Participants', 'counts': [{'value': '14', 'groupId': 'BG000'}]}], 'categories': [{'title': 'Female', 'measurements': [{'value': '0', 'groupId': 'BG000'}]}, {'title': 'Male', 'measurements': [{'value': '14', 'groupId': 'BG000'}]}]}], 'paramType': 'COUNT_OF_PARTICIPANTS', 'unitOfMeasure': 'Participants'}, {'title': 'Race and Ethnicity Not Collected', 'classes': [{'denoms': [{'units': 'Participants', 'counts': [{'value': '0', 'groupId': 'BG000'}]}]}], 'paramType': 'COUNT_OF_PARTICIPANTS', 'unitOfMeasure': 'Participants', 'populationDescription': 'Race and Ethnicity were not collected from any participant.'}, {'title': 'Region of Enrollment', 'classes': [{'title': 'United States', 'denoms': [{'units': 'Participants', 'counts': [{'value': '14', 'groupId': 'BG000'}]}], 'categories': [{'measurements': [{'value': '14', 'groupId': 'BG000'}]}]}], 'paramType': 'NUMBER', 'unitOfMeasure': 'participants'}, {'title': 'Height', 'classes': [{'denoms': [{'units': 'Participants', 'counts': [{'value': '14', 'groupId': 'BG000'}]}], 'categories': [{'measurements': [{'value': '179.2', 'spread': '6.2', 'groupId': 'BG000'}]}]}], 'paramType': 'MEAN', 'unitOfMeasure': 'centimeters', 'dispersionType': 'STANDARD_DEVIATION'}, {'title': 'Weight', 'classes': [{'denoms': [{'units': 'Participants', 'counts': [{'value': '14', 'groupId': 'BG000'}]}], 'categories': [{'measurements': [{'value': '82.6', 'spread': '15.9', 'groupId': 'BG000'}]}]}], 'paramType': 'MEAN', 'unitOfMeasure': 'kilograms', 'dispersionType': 'STANDARD_DEVIATION'}, {'title': 'Body Mass Index', 'classes': [{'denoms': [{'units': 'Participants', 'counts': [{'value': '14', 'groupId': 'BG000'}]}], 'categories': [{'measurements': [{'value': '25.8', 'spread': '5.0', 'groupId': 'BG000'}]}]}], 'paramType': 'MEAN', 'unitOfMeasure': 'kilograms per square meter', 'dispersionType': 'STANDARD_DEVIATION'}, {'title': 'Perceived Exercise Intensity', 'classes': [{'denoms': [{'units': 'Participants', 'counts': [{'value': '14', 'groupId': 'BG000'}]}], 'categories': [{'measurements': [{'value': '1.6', 'spread': '0.5', 'groupId': 'BG000'}]}]}], 'paramType': 'MEAN', 'description': 'Based on Physical Activity Intensity guidelines published by the Centers for Disease Control and Prevention. Intensity is based on an arbitrary scale where 0 = Sedentary, 1 = Light, 2 = Moderate, 3 = Strenuous', 'unitOfMeasure': 'units on a scale', 'dispersionType': 'STANDARD_DEVIATION'}]}}, 'documentSection': {'largeDocumentModule': {'largeDocs': [{'date': '2017-02-09', 'size': 221028, 'label': 'Study Protocol', 'hasIcf': False, 'hasSap': False, 'filename': 'Prot_000.pdf', 'typeAbbrev': 'Prot', 'uploadDate': '2017-09-29T16:17', 'hasProtocol': True}, {'date': '2017-02-13', 'size': 193099, 'label': 'Informed Consent Form', 'hasIcf': True, 'hasSap': False, 'filename': 'ICF_001.pdf', 'typeAbbrev': 'ICF', 'uploadDate': '2017-09-29T16:18', 'hasProtocol': False}, {'date': '2017-12-05', 'size': 128959, 'label': 'Statistical Analysis Plan', 'hasIcf': False, 'hasSap': True, 'filename': 'SAP_002.pdf', 'typeAbbrev': 'SAP', 'uploadDate': '2017-12-11T17:42', 'hasProtocol': False}]}}, 'protocolSection': {'designModule': {'phases': ['NA'], 'studyType': 'INTERVENTIONAL', 'designInfo': {'allocation': 'NA', 'maskingInfo': {'masking': 'NONE'}, 'primaryPurpose': 'BASIC_SCIENCE', 'interventionModel': 'SINGLE_GROUP'}, 'enrollmentInfo': {'type': 'ACTUAL', 'count': 14}}, 'statusModule': {'overallStatus': 'COMPLETED', 'startDateStruct': {'date': '2016-11-28', 'type': 'ACTUAL'}, 'expandedAccessInfo': {'hasExpandedAccess': False}, 'statusVerifiedDate': '2018-04', 'completionDateStruct': {'date': '2017-06-08', 'type': 'ACTUAL'}, 'lastUpdateSubmitDate': '2018-04-30', 'studyFirstSubmitDate': '2016-10-12', 'resultsFirstSubmitDate': '2017-06-23', 'studyFirstSubmitQcDate': '2016-10-13', 'lastUpdatePostDateStruct': {'date': '2018-06-01', 'type': 'ACTUAL'}, 'resultsFirstSubmitQcDate': '2018-04-30', 'studyFirstPostDateStruct': {'date': '2016-10-18', 'type': 'ESTIMATED'}, 'resultsFirstPostDateStruct': {'date': '2018-06-01', 'type': 'ACTUAL'}, 'primaryCompletionDateStruct': {'date': '2017-06-08', 'type': 'ACTUAL'}}, 'outcomesModule': {'primaryOutcomes': [{'measure': 'Volar Forearm Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation', 'timeFrame': 'measured at study visit 1, 2 and 3; detected lipid mediator concentrations for study visit 1 reported', 'description': 'Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids.'}, {'measure': 'Volar Forearm Sweat Lipid Mediator Concentrations Following Exercise', 'timeFrame': 'measured at study visit 1, detected lipid mediator concentrations reported', 'description': 'Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids.'}, {'measure': 'Lower Back Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation', 'timeFrame': 'measured at study visit 1, detected lipid mediator concentrations reported', 'description': 'Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids.'}, {'measure': 'Anterior Distal Thigh Sweat Lipid Mediator Concentrations Following Pilocarpine Stimulation', 'timeFrame': 'measured at study visit 2, detected lipid mediator concentrations reported', 'description': 'Approximately 150 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids and endocannabinoid-like molecules, and sphingolipids.'}, {'measure': 'Change in Plasma Lipid Mediator Concentrations Before and After Oral Ibuprofen Administration', 'timeFrame': 'measured at four timepoints at study visit 4, detected lipid mediator concentrations for first timepoint reported', 'description': 'Approximately 100 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids, and endocannabinoid-like molecules.'}], 'secondaryOutcomes': [{'measure': 'Change in Pilocarpine-stimulated Sweat Lipid Mediator Concentrations Before and After Oral Ibuprofen Administration', 'timeFrame': 'measured at four timepoints at study visit 4, detected lipid mediator concentrations for first timepoint reported', 'description': 'Approximately 100 lipid mediators will be measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Assayed lipid mediators include oxygenated lipids, endocannabinoids, and endocannabinoid-like molecules.'}, {'measure': 'Change in Plasma Ibuprofen Concentrations', 'timeFrame': 'measured at four timepoints at study visit 4', 'description': 'Plasma concentration of ibuprofen prior to and 30 min, 2 hr and 4 hr after oral administration'}, {'measure': 'Change in Sweat Ibuprofen Concentrations', 'timeFrame': 'measured at four timepoints at study visit 4', 'description': 'Sweat concentration of ibuprofen prior to and 30 min, 2 hr and 4 hr after oral administration'}]}, 'oversightModule': {'oversightHasDmc': False}, 'conditionsModule': {'keywords': ['oxygenated lipids', 'metabolic profiling', 'sphingoid bases', 'ceramides', 'endocannabinoids', 'non-invasive sampling'], 'conditions': ['Stability & Variability of Lipid-derived Molecules in Sweat']}, 'descriptionModule': {'briefSummary': 'The purpose of this study is to see what the differences are in sweat (amount and small molecule content) collected from different sites of the body and by different methods of sweat stimulation. Additionally, the investigators want to know whether the amount and small molecule content of the sweat is the same in an individual over time, and the same across individuals at a given time. Finally, the investigators want to know how consumption of over-the-counter anti-inflammatory drugs such as ibuprofen will affect the inflammatory mediator content of sweat and how that compares to blood. This information will help to better understand the composition and behavior of sweat and assess its potential utility as a routine clinical tool in skin research.', 'detailedDescription': 'Subjects will participate in 4 study day visits that will be scheduled about 1- week apart. During the first study visit, the investigators will compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological stimulation of sweating (using a stationary bicycle). During the second study visit, the investigators will collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) and also the upper surface of the thigh (near the knee). During the third study visit, the investigators will collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist). During the fourth study visit, the investigators will collect a sweat sample following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) and a blood sample (about one teaspoon) from the other arm. After blood and sweat collection, participants will consume 400 mg (two tablets) of ibuprofen. The investigators will then collect blood and sweat from 30 minutes, 2 hours and 4 hours after participants consume the ibuprofen.'}, 'eligibilityModule': {'sex': 'MALE', 'stdAges': ['ADULT'], 'maximumAge': '40 Years', 'minimumAge': '20 Years', 'healthyVolunteers': True, 'eligibilityCriteria': 'Inclusion Criteria:\n\n* 20-40 y\n* Male\n* Weight \\> 110 pounds\n\nExclusion Criteria:\n\n* Diagnosed active chronic diseases for which the individual is currently taking daily medication, including but not limited to:\n\n * Diabetes mellitus\n * Cardiovascular disease\n * Cancer\n * Gastrointestinal disorders\n * Kidney disease\n * Liver disease\n * Bleeding disorders\n * Asthma\n * Autoimmune disorders\n * Hypertension\n * Osteoporosis\n* Recent minor surgery (within 4 wk) or major surgery (within 16 wk)\n* Recent antibiotic therapy (within 4 wk)\n* Recent hospitalization (within 4 wk)\n* Use of over-the-counter or prescription medications at the time of the study that directly affect endpoints of interest (e.g. hyperlipidemia, glycemic control, steroids, statins, anti-inflammatory agents, and weight loss aids)\n* Adults who are not able to consent\n* Under current medical supervision\n* Ibuprofen intolerance or allergy\n* Those with a bleeding disorder\n* Current enrollee in a clinical research study.\n* Individuals with blood clotting or platelet defect disorders\n* Individuals with orthopedic limitations or cardiovascular risk that preclude participation in the physiological stimulation of sweat by light exercise portion of the study\n* Individuals who are trained athletes or that regularly perform physical activity defined as "vigorous" by the Centers for Disease Control and Prevention'}, 'identificationModule': {'nctId': 'NCT02935894', 'briefTitle': 'Investigating the Stability, Variability and Mechanism of Incorporation of Lipid Mediators Into Eccrine Sweat', 'organization': {'class': 'FED', 'fullName': 'USDA, Western Human Nutrition Research Center'}, 'officialTitle': 'Investigating the Stability, Variability and Mechanism of Incorporation of Lipid Mediators Into Eccrine Sweat', 'orgStudyIdInfo': {'id': '929370'}}, 'armsInterventionsModule': {'armGroups': [{'type': 'EXPERIMENTAL', 'label': 'Single group', 'description': 'All subjects will participate in 4 study day visits in the same order.\n\n1. compare sweat collected following pharmacological stimulation of sweating (using the drug pilocarpine) to sweat collected following physiological induction of sweating (exercise using a stationary bicycle).\n2. compare sweat collected following stimulation of sweating by the drug pilocarpine from the inner part of the forearm (near the wrist) to upper surface of thigh (near the knee).\n3. collect sweat following stimulation of sweating by the drug pilocarpine from the inner part of both forearms (near the wrist).\n4. collect blood and sweat samples before and 30 minutes, 2 hours and 4 hours after consumption of 400 mg of ibuprofen.', 'interventionNames': ['Drug: Ibuprofen', 'Other: Physiological induction of sweating', 'Drug: Pilocarpine']}], 'interventions': [{'name': 'Ibuprofen', 'type': 'DRUG', 'description': '400 mg ibuprofen given to inhibit cyclooxygenase metabolism', 'armGroupLabels': ['Single group']}, {'name': 'Physiological induction of sweating', 'type': 'OTHER', 'otherNames': ['Exercise'], 'description': '15 minutes exercise at 60-80% oxygen consumption to induce sweating', 'armGroupLabels': ['Single group']}, {'name': 'Pilocarpine', 'type': 'DRUG', 'otherNames': ['Pilogel'], 'description': 'Iontophoresis with a 1.5 milliampere (mA) current using a gel disc containing a 5% pilocarpine nitrate solution to induce sweating', 'armGroupLabels': ['Single group']}]}, 'contactsLocationsModule': {'locations': [{'zip': '95616', 'city': 'Davis', 'state': 'California', 'country': 'United States', 'facility': 'Western Human Nutrition Research Center', 'geoPoint': {'lat': 38.54491, 'lon': -121.74052}}], 'overallOfficials': [{'name': 'John W. Newman, Ph.D.', 'role': 'PRINCIPAL_INVESTIGATOR', 'affiliation': 'USDA-ARS-Western Human Nutrition Research Center'}]}, 'ipdSharingStatementModule': {'ipdSharing': 'NO'}, 'sponsorCollaboratorsModule': {'leadSponsor': {'name': 'USDA, Western Human Nutrition Research Center', 'class': 'FED'}, 'collaborators': [{'name': 'University of California, Davis', 'class': 'OTHER'}], 'responsibleParty': {'type': 'SPONSOR'}}}}