Ignite Creation Date:
2024-06-16 @ 11:51 AM
Last Modification Date:
2024-10-26 @ 3:31 PM
Study NCT ID:
NCT06450301
Status:
RECRUITING
Last Update Posted:
2024-06-11
First Post:
2024-05-28
Brief Title:
Senescent Endometrial Cells in Patients With Thin Endometrial Lining
Sponsor:
Organization:
Trio Fertility
Study Design
Study Type:
OBSERVATIONAL
Expanded Access Type Individual:
None
Expanded Access Type Intermediate:
None
Expanded Access Type Treatment:
None
Patient Registry:
False
Target Duration:
None
Design Allocation:
Design Intervention Model:
Design Intervention Model Description:
Design Masking Description:
Bio Spec Retention:
SAMPLES_WITH_DNA
Bio Spec Description:
We will measure the percentage of senescent cells in endometrial biopsies by Sudan Black-B SBB immunohistochemistry since this is a specific marker of cell senescence and can be performed on formalin fixed paraffin-embedded tissue This assay also allows us to test archived samples for women in the control group who have already had an endometrial biopsy We will also freeze part of the endometrial biopsy sample for RNA extraction for senescent biomarker expression BMI-1 p16lnka4 p14ARF Cyclin-D1 and CDK4 by RT-PCR and protein determination western blotting for confirmation of the SBB staining results
Enrollment Count:
100
Enrollment Type:
ESTIMATED
Design Primary Purpose:
Design Masking:
Phases:
Observational Models:
| Name |
|---|
| Cohort |
Time Perspective List:
Clinical Trial Statuses
| Name |
|---|
| 107695 |
Who Masked List: