Ignite Creation Date:
2024-05-06 @ 7:07 PM
Last Modification Date:
2024-10-26 @ 3:00 PM
Study NCT ID:
NCT05894512
Status:
ACTIVE_NOT_RECRUITING
Last Update Posted:
2024-04-30
First Post:
2023-05-31
Brief Title:
Analysis of T- and B-Cell Subpopulations in Membranous Nephropathy
Sponsor:
Organization:
Istanbul University
Study Design
Study Type:
OBSERVATIONAL
Expanded Access Type Individual:
None
Expanded Access Type Intermediate:
None
Expanded Access Type Treatment:
None
Patient Registry:
False
Target Duration:
None
Design Allocation:
Design Intervention Model:
Design Intervention Model Description:
Design Masking Description:
Bio Spec Retention:
SAMPLES_WITH_DNA
Bio Spec Description:
Peripheral venous blood samples from the volunteers will be taken into 20 ml EDTA heparinized and dry tubes Blood samples taken into a 10 ml dry tube will be centrifuged at 3000 rpm for 10 minutes After the process the separated serum will be placed in Eppendorf tubes microcentrifuge tubes and frozen at -20C for 48 hours and then transferred to deep freezers to be stored at -80C
Peripheral blood mononuclear cells PBMCs will be obtained from blood samples by Ficoll concentration gradient centrifugation method PBMCs collected after centrifugation will be suspended with fetal bovine serum FBS Cells in FBS medium will be taken into cryotubes frozen in liquid nitrogen -196C by adding dimethyl sulfoxide and stored until the laboratory studies
Enrollment Count:
50
Enrollment Type:
ESTIMATED
Design Primary Purpose:
Design Masking:
Phases:
Observational Models:
| Name |
|---|
| Cohort |
Time Perspective List:
Clinical Trial Statuses
| Name |
|---|
| 96248 |
Who Masked List: