Ignite Creation Date:
2024-05-06 @ 4:52 PM
Last Modification Date:
2024-10-26 @ 2:17 PM
Study NCT ID:
NCT05118126
Status:
UNKNOWN
Last Update Posted:
2022-01-12
First Post:
2021-11-01
Brief Title:
Gut Microbiota in ITP
Sponsor:
Organization:
Peking University Peoples Hospital
Study Design
Study Type:
OBSERVATIONAL
Expanded Access Type Individual:
None
Expanded Access Type Intermediate:
None
Expanded Access Type Treatment:
None
Patient Registry:
False
Target Duration:
None
Design Allocation:
Design Intervention Model:
Design Intervention Model Description:
Design Masking Description:
Bio Spec Retention:
SAMPLES_WITH_DNA
Bio Spec Description:
Fecal sample collection and metagenomic sequencing A specific stool sample collector was selected to collect an appropriate amount of stool samples 1g sample Samples that could not be extracted immediately were transported on dry ice and stored at -80 DNA was extracted by phenolchloroform method Qubit Fluorometer is used to test samples DNA concentration agarose gel electrophoresis gel concentration 1 Voltage 150 V Electrophoresis time 40 min is used to test DNA integrity of samples and unqualified samples with insufficient DNA amount or degradation were eliminated Qualified samples were used for DNA library construction and Illumina sequencing The sequencing library was constructed according to Illuminas official operating instructions and further optimized Qualified libraries were sequenced using Illumina platform
Enrollment Count:
240
Enrollment Type:
ESTIMATED
Design Primary Purpose:
Design Masking:
Phases:
Observational Models:
| Name |
|---|
| Cohort |
Time Perspective List:
Clinical Trial Statuses
| Name |
|---|
| 80437 |
Who Masked List: