Ignite Creation Date:
2024-05-06 @ 2:57 PM
Last Modification Date:
2024-10-26 @ 1:40 PM
Study NCT ID:
NCT04478409
Status:
RECRUITING
Last Update Posted:
2021-11-22
First Post:
2020-07-16
Brief Title:
Characterization of a Functional Test for Mediterranean Family Fever Screening - 2
Sponsor:
Organization:
Hospices Civils de Lyon
Study Design
Study Type:
OBSERVATIONAL
Expanded Access Type Individual:
None
Expanded Access Type Intermediate:
None
Expanded Access Type Treatment:
None
Patient Registry:
False
Target Duration:
None
Design Allocation:
Design Intervention Model:
Design Intervention Model Description:
Design Masking Description:
Bio Spec Retention:
SAMPLES_WITHOUT_DNA
Bio Spec Description:
Sample will be code and sent to the laboratory within 24 hours Inserm U1111 Lyon For healthy subjects research does not involve drawing additional blood from their blood donation
There will be no long-term storage or establishment of biological collections The monocytes will be extracted selected and counted The pyras inflammasome will then be activated and the interleukin-1β secretion will be measured by ELISA Cell death will be quantified in real time by fluorimetry
Validation on cellular models in vitro immortalized monocytes will be invalidated for the MEFV gene by CrispR-Cas9 and different inducible variants of the MEFV gene will be expressed in MEFV knot out cells These genetically modified cells will then undergo the tests for stimulation of the inflammatory Pyrine according to the same procedures as the monocytes of the patients
Enrollment Count:
160
Enrollment Type:
ESTIMATED
Design Primary Purpose:
Design Masking:
Phases:
Observational Models:
| Name |
|---|
| Case-Control |
Time Perspective List:
Clinical Trial Statuses
| Name |
|---|
| 66909 |
Who Masked List: