Ignite Creation Date:
2024-05-06 @ 10:40 AM
Last Modification Date:
2024-10-26 @ 12:34 PM
Study NCT ID:
NCT03326037
Status:
UNKNOWN
Last Update Posted:
2019-01-10
First Post:
2017-10-22
Brief Title:
Study of The Association of Mutations in The NPHS2 Gene and Nephrotic Syndrome in Children and Adults in Middle East
Sponsor:
Organization:
Assiut University
Study Design
Study Type:
OBSERVATIONAL
Expanded Access Type Individual:
None
Expanded Access Type Intermediate:
None
Expanded Access Type Treatment:
None
Patient Registry:
True
Target Duration:
2 Years
Design Allocation:
Design Intervention Model:
Design Intervention Model Description:
Design Masking Description:
Bio Spec Retention:
SAMPLES_WITH_DNA
Bio Spec Description:
Saliva and blood sampling and DNA extraction
After obtaining informed consent from parents of children and adults 3 ml of blood will be collected from children and in the event that the patients or their parents refuse then buccal swabs will be collected from children and 5ml of blood will be collected from adults The buccal swabs will be processed to extract DNA and stored at -20 till time of genetic analysis Peripheral blood samples will be collected in EDTA anticoagulated tubes and DNA will be extracted according to standard methods using QIAGEN DNA blood mini kit QIAGEN and stored at 20 till time of analysis
Genotyping Genotyping of the NPHS2 variant rs61747728 will be performed by Real-time TaqMan Allelic Discrimination Assay Life technologies CA USA according to standard manufacturer protocols Allelic discrimination analysis will be performed and analyzed using ABI 7500 Fast Real-time PCR system SDS software Life technologies CA USA
Enrollment Count:
150
Enrollment Type:
ESTIMATED
Design Primary Purpose:
Design Masking:
Phases:
Observational Models:
| Name |
|---|
| Cohort |
Time Perspective List:
Clinical Trial Statuses
| Name |
|---|
| 44505 |
Who Masked List: