Ignite Creation Date:
2024-05-05 @ 10:14 PM
Last Modification Date:
2024-10-26 @ 10:16 AM
Study NCT ID:
NCT01066533
Status:
TERMINATED
Last Update Posted:
2010-02-10
First Post:
2010-02-09
Brief Title:
Immunohistochemical Study of Human Teeth Pulp Capped With Mineral Trioxide Aggregate MTA and Novel Endodontic Cement NEC
Sponsor:
Mashhad University of Medical Sciences
Organization:
Mashhad University of Medical Sciences
Study Overview
Official Title:
Histologic and Immunohistochemical Study of Fibronectin and Tenascin in Human Teeth Pulp Capped With MTA and NEC
Status:
TERMINATED
Status Verified Date:
2009-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
immunohistochemistry markers are not recieved yet
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The purpose of this study is to evaluate the response of human dental pulp to capping with MTA and NEC by histology and immunohistochemistry using fibronectin and tenascin as markers following 2 weeks and 8 weeks
Detailed Description:
Capping of the exposed pulp is indicated for reversible pulp tissue injury after physical or mechanical trauma in developing or mature teethThe response to direct pulp capping with materials such as MTA or NEC is the formation of dentin barrier resulting from the recruitment and proliferation of undifferentiated cells
Different materials can be used to induce dentinal bridge formationMTA has been introduced as a proper material to induce dentinal bridge formationwith greater mean thickness of dentinal bridge and less inflammation compared to traditional materials like calcium hydroxide Although biocompatible MTA has a poor handling characteristic delay setting time off-white color and it is almost expensiveThe Novel Endodontic Cement NEC has been introduced to combine reasonable characteristics of MTA with appropriate chemical properties setting time color and handling characteristics
The extracellular matrix ECM of pulp comprises a variety of proteins and polysaccharides forming a neat network The ECM components can induce either reactionary or reparative dentin formation Fibronectin FN and tenascin TNC are the two major glycoproteins involved in wound healing and odontogenesis
Fibronectins FNs are a class of high molecular weight adhesive proteins composed of two very large subunits It has a variety of cell functions such as adhesion migration growth and differentiation Furthermore FN may be involved in the polarization and migration of odontoblasts
Tenascin is a large oligomeric glycoprotein of the ECM secreted by fibroblasts and glial cells in tissue cultures It has also been identified in pulp and more prominent in dentinogenesis TN has been shown to be important in the differentiation of odontoblasts Therefore it may be associated with secondary dentin formation when pulp cells differentiate into odontoblasts in response to physiologic stimuli
Although dentin bridge formation has been reportedusing different materials such as MTA and NEC in direct pulp cap but the exact mechanism is not well understood Immunohistochemistry using different markers may be useful in developing the molecules involved in this process
Moreoverresponse of dental pulp to different pulp capping materials can be studiedincluding mean thickness of dentinal bridge morphology of dentinal bridge and intensity of pulp inflammation and presence of odontoblast cells
Different materials can be used to induce dentinal bridge formationMTA has been introduced as a proper material to induce dentinal bridge formationwith greater mean thickness of dentinal bridge and less inflammation compared to traditional materials like calcium hydroxide Although biocompatible MTA has a poor handling characteristic delay setting time off-white color and it is almost expensiveThe Novel Endodontic Cement NEC has been introduced to combine reasonable characteristics of MTA with appropriate chemical properties setting time color and handling characteristics
The extracellular matrix ECM of pulp comprises a variety of proteins and polysaccharides forming a neat network The ECM components can induce either reactionary or reparative dentin formation Fibronectin FN and tenascin TNC are the two major glycoproteins involved in wound healing and odontogenesis
Fibronectins FNs are a class of high molecular weight adhesive proteins composed of two very large subunits It has a variety of cell functions such as adhesion migration growth and differentiation Furthermore FN may be involved in the polarization and migration of odontoblasts
Tenascin is a large oligomeric glycoprotein of the ECM secreted by fibroblasts and glial cells in tissue cultures It has also been identified in pulp and more prominent in dentinogenesis TN has been shown to be important in the differentiation of odontoblasts Therefore it may be associated with secondary dentin formation when pulp cells differentiate into odontoblasts in response to physiologic stimuli
Although dentin bridge formation has been reportedusing different materials such as MTA and NEC in direct pulp cap but the exact mechanism is not well understood Immunohistochemistry using different markers may be useful in developing the molecules involved in this process
Moreoverresponse of dental pulp to different pulp capping materials can be studiedincluding mean thickness of dentinal bridge morphology of dentinal bridge and intensity of pulp inflammation and presence of odontoblast cells
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None