Ignite Creation Date:
2024-10-26 @ 3:43 PM
Last Modification Date:
2024-10-26 @ 3:43 PM
Study NCT ID:
NCT06657755
Status:
COMPLETED
Last Update Posted:
None
First Post:
2024-10-21
Brief Title:
Evaluation of ENPP1 Expression and Immune Response in Bladder Cancer Patients
Sponsor:
None
Organization:
None
Study Overview
Official Title:
A Study to Evaluate ENPP1 Expression and Its Role in Immune Evasion and Chemoresistance in Bladder Cancer
Status:
COMPLETED
Status Verified Date:
2024-10
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
ENPP1-BC
Brief Summary:
This study aims to investigate the role of ENPP1 in bladder cancer BC specifically focusing on its impact on immune evasion chemoresistance and prognosis The study will analyze gene expression data from clinical samples and use various laboratory techniques including RNA sequencing qRT-PCR and immunohistochemistry to assess ENPP1s expression levels In addition the research will explore the relationship between ENPP1 and immune cell infiltration along with its correlation with patient survival outcomes By identifying ENPP1s contribution to cancer progression and treatment resistance the study aims to discover potential therapeutic targets for improving bladder cancer treatment strategies
Detailed Description:
This study is designed to comprehensively explore the role of ENPP1 Ectonucleotide PyrophosphatasePhosphodiesterase 1 in bladder cancer BC development and progression ENPP1 has been implicated in various cancers with evidence suggesting its involvement in immune evasion chemoresistance and poor prognosis However its specific function in bladder cancer remains unclear This research will analyze clinical samples including tumor tissues and normal adjacent tissues to evaluate ENPP1 expression through a combination of advanced laboratory techniques such as RNA sequencing quantitative real-time PCR qRT-PCR Western blotting immunohistochemistry IHC and immunofluorescence
Furthermore the study will investigate the association between ENPP1 expression and key clinical parameters including tumor stage grade lymph node involvement metastasis and patient survival outcomes Through bioinformatics tools immune infiltration analysis will be conducted to assess the impact of ENPP1 on immune cells such as CD8 T cells dendritic cells and M2 macrophages which are crucial in the tumor microenvironment
The research will also explore the correlation between ENPP1 expression and resistance to common chemotherapeutic agents particularly gemcitabine and cytarabine using drug sensitivity data from public databases By identifying the molecular mechanisms by which ENPP1 influences immune responses and chemoresistance the study aims to provide valuable insights into potential therapeutic targets for bladder cancer treatment Ultimately this research may contribute to the development of personalized therapies for bladder cancer patients based on ENPP1 expression
Furthermore the study will investigate the association between ENPP1 expression and key clinical parameters including tumor stage grade lymph node involvement metastasis and patient survival outcomes Through bioinformatics tools immune infiltration analysis will be conducted to assess the impact of ENPP1 on immune cells such as CD8 T cells dendritic cells and M2 macrophages which are crucial in the tumor microenvironment
The research will also explore the correlation between ENPP1 expression and resistance to common chemotherapeutic agents particularly gemcitabine and cytarabine using drug sensitivity data from public databases By identifying the molecular mechanisms by which ENPP1 influences immune responses and chemoresistance the study aims to provide valuable insights into potential therapeutic targets for bladder cancer treatment Ultimately this research may contribute to the development of personalized therapies for bladder cancer patients based on ENPP1 expression
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None