Ignite Creation Date:
2024-10-26 @ 3:38 PM
Last Modification Date:
2024-10-26 @ 3:38 PM
Study NCT ID:
NCT06561893
Status:
NOT_YET_RECRUITING
Last Update Posted:
None
First Post:
2024-06-18
Brief Title:
Effect of L Plantarum Probiotic Supplementation on Broccoli Sulforaphane Bioavailability Randomised Double-blind Placebo-controlled Crossover Study
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Effect of L Plantarum Probiotic Supplementation on Broccoli Sulforaphane Bioavailability Randomised Double-blind Placebo-controlled Crossover Study
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-08
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
PRO-BROC
Brief Summary:
Broccoli has the precursor of an active compound sulforaphane that has shown a wide range of health promoting benefits Sulforaphane formation depends on the conversion of glucoraphanin precursor by myrosinase enzyme Thus the bioavailability of sulforaphane is affected by myrosinase activity Some bacteria such as L plantarum probiotic have shown they can also convert glucoraphanin to sulforaphane in vitro This study investigates the effect of short-term L plantarum supplementation on broccoli sulforaphane bioavailability in humans using a randomised double-blind placebo-controlled crossover trial
Detailed Description:
Brassicas ie broccoli kale cabbage are a group of edible plants that contain a phytochemical called glucoraphanin that upon enzymatic hydrolysis forms the bioactive form sulforaphane Sulforaphane has shown consistent in vitro and in vivo animals anti-cancer activity The enzyme responsible for this conversion is called myrosinase however the enzymatic activity of myrosinase can be affected by several biological factors eg plant variety growth conditions etc and food processing eg cooking drying etc Broccoli is one of the richest sources of glucoraphanin but its myrosinase activity can be easily lost during cooking 10 minutes at 70 C reduces the enzyme activity by 95 This means broccoli prepared this way will contain little to no biologically active sulforaphane Therefore is important to find strategies to increase the bioavailability of sulforaphane in broccoli and other brassicas for human consumption
Certain bacteria have shown myrosinase-like activity and were able to convert glucoraphanin to sulforaphane in vitro one of these bacteria is Lactiplantibacillus plantarum This bacterium is present in the human gut however there is a large biological variability in the prevalence of this bacteria between individuals Several human studies have demonstrated that chronic ampampgt1 week consumption of probiotic supplements containing L plantarum was able to increase the presence of this bacterium in human gut participants Thus the investigators aim to test if supplementing participants with L plantarum will increase the bioavailability of sulforaphane compared to placebo
Certain bacteria have shown myrosinase-like activity and were able to convert glucoraphanin to sulforaphane in vitro one of these bacteria is Lactiplantibacillus plantarum This bacterium is present in the human gut however there is a large biological variability in the prevalence of this bacteria between individuals Several human studies have demonstrated that chronic ampampgt1 week consumption of probiotic supplements containing L plantarum was able to increase the presence of this bacterium in human gut participants Thus the investigators aim to test if supplementing participants with L plantarum will increase the bioavailability of sulforaphane compared to placebo
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None