Ignite Creation Date:
2024-10-26 @ 3:38 PM
Last Modification Date:
2024-10-26 @ 3:38 PM
Study NCT ID:
NCT06560216
Status:
NOT_YET_RECRUITING
Last Update Posted:
None
First Post:
2024-08-15
Brief Title:
Cryopreservation Medium With Apigenin on Post-thaw Human Sperm
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-08
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The goal of this clinical trial is to compare the effect of apigenin supplementation in freezing medium with no apigenin supplementation in freezing medium on post-thaw human sperm motility It will also evaluate the sperm viability total antioxidant capacity and oxidative stress markers The main questions it aims to answer are
Does apigenin supplementation in freezing medium improve the post-thaw sperm motility
Does apigenin supplementation in freezing medium improve the post-thaw sperm viability total antioxidant capacity and oxidative stress
Does apigenin supplementation in freezing medium improve the post-thaw sperm motility
Does apigenin supplementation in freezing medium improve the post-thaw sperm viability total antioxidant capacity and oxidative stress
Detailed Description:
All normal semen samples from participants who give written informed consent and meet the eligibility requirements will be included in the study Prepared semen samples were divided into 3 groups
1 Pre-freezing sperm suspension
2 Frozen-thawed control without apigenin supplementation in freezing medium
3 Frozen-thawed sperm suspension with apigenin 02 mM supplementation in freezing medium Baseline sperm motility will be measured in pre-freezing sperm suspension The last 2 groups will be stored in liquid nitrogen for at least one week After thawing the sperm suspensions will be evaluated for motility viability total antioxidant capacity and oxidative stress markers
1 Pre-freezing sperm suspension
2 Frozen-thawed control without apigenin supplementation in freezing medium
3 Frozen-thawed sperm suspension with apigenin 02 mM supplementation in freezing medium Baseline sperm motility will be measured in pre-freezing sperm suspension The last 2 groups will be stored in liquid nitrogen for at least one week After thawing the sperm suspensions will be evaluated for motility viability total antioxidant capacity and oxidative stress markers
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None