Ignite Creation Date:
2024-10-26 @ 3:37 PM
Last Modification Date:
2024-10-26 @ 3:37 PM
Study NCT ID:
NCT06542042
Status:
NOT_YET_RECRUITING
Last Update Posted:
None
First Post:
2024-06-27
Brief Title:
Application of Nanopore Adaptive Sequencing
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Application of Oxford Nanopore Adaptive Sequencing Method in Infection Post Liver Transplantation
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-06
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Infection post liver transplantation is an important factor in the death in patients The traditional method of diagnosing infection post liver transplantation is laboratory tests But the sensitivity and specificity of blood tests is poor Next-generation sequencing NGS has greater detection rate for mycobacterium tuberculosis anaerobes and fungi and greater sensitivity compare with blood tests However use of NGS is limited because of the short read-length Oxford nanopore adaptive sequencing NAS method is the Third Revolution in Sequencing Technology For each 1 Gbp of data NAS sequencing detected 45 times more microbiome sequences than Nanopore standard sequencing and 25 times more than Illumina sequencing The purpose of this study is to compare NAS with NGS and laboratory tests for the diagnostic rate of infection post liver transplantation
Detailed Description:
Based on the previous work the investigators found that 1 NAS has a higher microbial enrichment efficiency and can detect pathogenic information more quickly compared with nanopores normal sequencing NNS 2 NAS can detect more specific pathogen fragments with short-time sequencing 3 NAS can cover more pathogenic genomes than NNS in a short-time 4 NAS can detect antibiotic resistance information of pathogenic bacteria in clinical samples
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None