Ignite Creation Date:
2024-10-26 @ 3:35 PM
Last Modification Date:
2024-10-26 @ 3:35 PM
Study NCT ID:
NCT06514573
Status:
NOT_YET_RECRUITING
Last Update Posted:
None
First Post:
2024-06-26
Brief Title:
Butyrate Supplementation in Children With Autism Spectrum Disorder ASD and Functional Gastrointestinal Disorders
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Effects of a Postbiotic Supplementation on Gastrointestinal and Core Symptoms in Autism Spectrum Disorder A Multicenter Randomized Controlled Trial
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
bAUTyrate
Brief Summary:
Autism spectrum disorder ASD is a neurodevelopmental condition occurring in 177 Italian children Several comorbidities are reported including functional gastrointestinal disorders FGIDs present in up to 70 of patients FGIDs are disorders resulting from a combination of symptoms affecting motility hypersensitivity and other functions which are not caused by anatomic or organic origin and that impact the severity of ASD core symptoms and complicate the clinical management of ASD children especially those who are non-verbal Evidence reports gut microbiome GM remodelling in ASD children and postbiotic butyrate a GM-derived metabolite attenuates FGIDs in children and restores social behavior in ASD mouse models Clinical data on butyrate effects in ASD are still scanty The present study investigates the therapeutic effects of a 16-week oral postbiotic supplementation on clinicalbehavioral profiles gastrointestinal disturbances gut microbiome and immune and inflammatory biomarkers in peripheral blood and fecal samples in children with ASD and FGIDs Using the Machine Learning ML approach a subset of artificial intelligence this study also aims to identify predictive factors implicated in the effect of the postbiotic supplementation on FGIDs important for prevention through modulation of the microbiota The investigators expect that treating FGIDs will have an impact on the behavioral and core symptoms of ASD and the quality of life of children and their families
Detailed Description:
ASD is a heterogeneous group of neurodevelopmental disorders with complex multifactorial etiologies requiring personalized timely and evidence-based interventions to improve the lives of children and their families Medical comorbidities are common in ASD and include FGIDs which are disorders resulting from a combination of symptoms affecting motility hypersensitivity and other functions which are not caused by anatomic or organic origin FGIDs are often associated with sleep and behavioral problems with a negative impact on clinical functioning of ASD children Pharmacological agents that could target FGIDs in ASD children are advocated Immune system epigenetic gut microbiome mitochondrial metabolism and gut-brain axis alterations have been implicated in ASD and FGIDs etiology and may represent potential intervention targets The term postbiotics can be regarded as an umbrella term for all synonyms and related terms of microbial fermentation components including many different constituents such as short-chain fatty acids microbial cell fractions functional proteins extracellular polysaccharides cell lysates teichoic acid peptidoglycan-derived muropeptides and pili-type structures Emerging data indicate that postbiotics can have direct immunomodulatory and clinically relevant effects Evidence can be found for using postbiotics in healthy individuals to improve overall health and relieve symptoms of various diseases In particular the postbiotic butyrate a Gut Microbiota-derived short-chain fatty acid exerts protective action against FGIDs and ASD through a wide range of activities involving Gut Microbiota immune system epigenetic mechanisms and mitochondrial function Taken together these data strongly support the hypothesis that butyrate oral supplementation can exert a therapeutic action against FGIDs and behavioral symptoms in ASD children Butyrate has been proposed for FGIDs treatment but its promising effect on ASD is limited to preclinical data The present multicenter double blinded randomized controlled trial has been rigorously designed to investigate the therapeutic effects of oral butyrate supplementation in children with ASD either idiopathic or syndromic and FGIDs In line with precision medicine and designing and improving diagnosis therapeutics and prognosis using large complex datasets this study will implement high-performance computing and artificial intelligence in multidimensional clinical and biological datasets The great heterogeneity of ASD in terms of etiology and clinical presentation requires a better understanding of who is most likely to respond to which interventions at what intensity and for what duration so they could be assigned to those most likely to benefit while reducing the cost of treatment to the National Health Service Thus there is a need to identify mediators of treatment response and moderators factors that predict greater responsiveness to treatment including factors that influence individuals responses to various treatments allowing clinicians to tailor early interventions more carefully To this aim mediators and moderators implicated in the effect of postbiotic supplementation on FGIDs will be explored through ML performed on clinical and biological variables collected in the digital platform Research Electronic Data Capture REDCap Identifying patternsprofiles of the child responding to treatment may be important in assessing risk during the early stage and planning for individualized treatment and prevention of ASD through modulation of the microbiota
Increasing evidence suggests that the gut microbiome is one of the key modulators of gut-brain communication in ASD The brain-gut-microbiome axis has become a compelling area of investigation in ASD specifically in children Previous studies suggested the presence of alteration in gut microbiome structure and function in ASD children as well as in FGIDs decreased BacteroidetesFirmicutes well-known butyrate producers ratio in fecal samples of ASD children and significantly reduced butyrate level production by the gut microbiome of ASD children
Butyrate is a widely known histone deacetylase inhibitor due to its ability to cross the blood brain barrier Previous research has shown that treatment with oral sodium butyrate improved behavioral deficits in BTBR mice one of the most robust animal models of ASD The gene expression analyses of the frontal cortex revealed that the effects of butyrate on behavior were attributable to a positive modulation of genes involved in excitatoryinhibitory balance and neuronal activation Specifically sodium butyrate downregulated the neuronal activation marker genes and upregulated the inhibitory neurotransmitter genes In addition a double-blind trial in 3-8 year old children with ASD was recently conducted to assess the effect of L Reuteri supplementation 6 months on social deficits gastrointestinal GI symptoms and immune response Unpublished findings showed that L Reuteri significantly increased scores of Adaptive Behavior Assessment System social subdomains and decreased the total score of the Social Responsiveness Scale and gastrointestinal GI symptoms at 6 months Behavioral results based on intelligent quotient IQ stratification also revealed the L Reuteri efficacy on social and GI domains in ASD children with normal IQ range These studies highlight the promising role of probiotics and postbiotics in ASD children
Aims A multicenter double-blind sequential Randomized Controlled Trial of butyrate vs placebo will be carried out in 128 ASD children estimated sample size to evaluate the efficacy of the postbiotic supplementation on functional gastrointestinal disorders in children with ASD A secondary aim is to define whether and to what extent the butyrate supplementation could influence ASD behavioral and core symptoms The third aim is to identify clinical andor biological determinants of ASD childrens response to postbiotic supplementation
Materials and Methods Azienda Ospedaliera Universitaria Federico II of Naples and Policlinico Tor Vergata Hospital will invite parents of 3-6 years old ASD children to participate in the study If they agree full information about the protocol will be provided and written signed informed consent will be collected ASD diagnosis severity and clinical functioning will be defined according to Diagnostic and Statistical Manual of Mental Disorders DSM-5 criteria and gold-standard tests the FGIDs diagnosis will be performed using the Rome III Criteria questionnaire and the 6-GSI Childrens dietary habits will be assessed by the 3-day food diary Clinical centers will send age gender and IQ data to Istituto Superiore di Sanità for the centralized randomization procedure in the REDCap platform At baseline Timepoint 0 parents will be invited to Azienda Ospedaliera Universitaria Federico II of Naples and Policlinico Tor Vergata Hospital for a comprehensive clinical evaluation including anamnestic interview and the administration of standardized tests for autism and gastrointestinal symptoms global development and adaptive and behavioral functioning Blood and faecal samples for genetic and metabolic analysis will be collected and electroencephalography will also be performed
Parents will receive butyrate or placebo supplementation for 16 weeks 4 months according to the allocation process Treatment will be numbered according to the randomization scheme with no reference to group assignment only known by the Istituto Superiore di Sanità statistician who generates the list The Central Pharmacy Service of the Academic Hospital Federico II of Naples will prepare the sachets and indistinguishable same color smell taste treatments content Clinical centers will repeat a full evaluation at the end of the supplementation period Timepoint 1 At Timepoint 2 after 4 months by the end of Timepoint 1 parents will be asked to fill Timepoint 1 questionnaires to evaluate the long-term effect - consolidation - of supplementation To avoid further discomfort to children and families blood and fecal sampling will not be collected at Timepoint 2
Determinants of response to the butyrate supplementation and biomarkers that differentiate responders from non-responders will be identified by implementing high-performance computing and artificial intelligence to the multidimensional clinical and biological datasets collected in the digital platform developed and managed by Istituto Superiore di Sanità Using the ML approach a subset of artificial intelligence the University Federico II of Naples will analyze the dataset to establish relationships without preassigned modeling ML is an innovative approach to provide robust predictions overcoming descriptive statistical models and proving a digital twin for the considered phenomena Clinical behavioral inflammatory and immunological factors are altered in ASD However the great variability among individuals with ASD of these problems makes it difficult to predict who is most likely to respond to a specific intervention at what intensity and for what duration To this aim the investigators will develop a probabilistic multi-domain data integration model consisting of the gut microbiome immune and inflammatory biomarkers in peripheral blood and fecal samples and clinicalbehavioral biomarkers using ML to improve therapeutics and predict the prognosis of ASD Clinical and behavioral variables to be included in the dataset will be collected through the testsquestionnaires described in the experimental design Gut microbiome immune and inflammatory variables will be gathered on blood and fecal samples at baseline Timepoint 0 and at the end of supplementation Timepoint 1 The composition of the gut microbiome will be characterized by shotgun metagenomics Genomes of dominant strains will also be reconstructed with a recently validated standard pipeline and compared to define if a strain-level variation exists upon treatment with butyrate Fecal Short-Chain Fatty Acids SCFAs acetate propionate and butyrate levels will be determined by gas chromatograph-mass spectrometry as previously described Altered composition of the gut microbiome may alter the gut barrier potentially allowing the translocation of bacteria and their antigens toxins and metabolites and result in multiple alterations of cytokines production immune cell populations as well as cellular activation markers
Randomization and allocation concealment will be performed by the Istituto Superiore di Sanità Randomization will be generated using the procedure Ralloc in STATA managed through the RECap platform after enrollment and provided to clinicians by the Istituto Superiore di Sanità Specific standard operative procedures SOPs were defined to garantee study and trial integrity A blinded statistician will analyze data using Stata 161
Increasing evidence suggests that the gut microbiome is one of the key modulators of gut-brain communication in ASD The brain-gut-microbiome axis has become a compelling area of investigation in ASD specifically in children Previous studies suggested the presence of alteration in gut microbiome structure and function in ASD children as well as in FGIDs decreased BacteroidetesFirmicutes well-known butyrate producers ratio in fecal samples of ASD children and significantly reduced butyrate level production by the gut microbiome of ASD children
Butyrate is a widely known histone deacetylase inhibitor due to its ability to cross the blood brain barrier Previous research has shown that treatment with oral sodium butyrate improved behavioral deficits in BTBR mice one of the most robust animal models of ASD The gene expression analyses of the frontal cortex revealed that the effects of butyrate on behavior were attributable to a positive modulation of genes involved in excitatoryinhibitory balance and neuronal activation Specifically sodium butyrate downregulated the neuronal activation marker genes and upregulated the inhibitory neurotransmitter genes In addition a double-blind trial in 3-8 year old children with ASD was recently conducted to assess the effect of L Reuteri supplementation 6 months on social deficits gastrointestinal GI symptoms and immune response Unpublished findings showed that L Reuteri significantly increased scores of Adaptive Behavior Assessment System social subdomains and decreased the total score of the Social Responsiveness Scale and gastrointestinal GI symptoms at 6 months Behavioral results based on intelligent quotient IQ stratification also revealed the L Reuteri efficacy on social and GI domains in ASD children with normal IQ range These studies highlight the promising role of probiotics and postbiotics in ASD children
Aims A multicenter double-blind sequential Randomized Controlled Trial of butyrate vs placebo will be carried out in 128 ASD children estimated sample size to evaluate the efficacy of the postbiotic supplementation on functional gastrointestinal disorders in children with ASD A secondary aim is to define whether and to what extent the butyrate supplementation could influence ASD behavioral and core symptoms The third aim is to identify clinical andor biological determinants of ASD childrens response to postbiotic supplementation
Materials and Methods Azienda Ospedaliera Universitaria Federico II of Naples and Policlinico Tor Vergata Hospital will invite parents of 3-6 years old ASD children to participate in the study If they agree full information about the protocol will be provided and written signed informed consent will be collected ASD diagnosis severity and clinical functioning will be defined according to Diagnostic and Statistical Manual of Mental Disorders DSM-5 criteria and gold-standard tests the FGIDs diagnosis will be performed using the Rome III Criteria questionnaire and the 6-GSI Childrens dietary habits will be assessed by the 3-day food diary Clinical centers will send age gender and IQ data to Istituto Superiore di Sanità for the centralized randomization procedure in the REDCap platform At baseline Timepoint 0 parents will be invited to Azienda Ospedaliera Universitaria Federico II of Naples and Policlinico Tor Vergata Hospital for a comprehensive clinical evaluation including anamnestic interview and the administration of standardized tests for autism and gastrointestinal symptoms global development and adaptive and behavioral functioning Blood and faecal samples for genetic and metabolic analysis will be collected and electroencephalography will also be performed
Parents will receive butyrate or placebo supplementation for 16 weeks 4 months according to the allocation process Treatment will be numbered according to the randomization scheme with no reference to group assignment only known by the Istituto Superiore di Sanità statistician who generates the list The Central Pharmacy Service of the Academic Hospital Federico II of Naples will prepare the sachets and indistinguishable same color smell taste treatments content Clinical centers will repeat a full evaluation at the end of the supplementation period Timepoint 1 At Timepoint 2 after 4 months by the end of Timepoint 1 parents will be asked to fill Timepoint 1 questionnaires to evaluate the long-term effect - consolidation - of supplementation To avoid further discomfort to children and families blood and fecal sampling will not be collected at Timepoint 2
Determinants of response to the butyrate supplementation and biomarkers that differentiate responders from non-responders will be identified by implementing high-performance computing and artificial intelligence to the multidimensional clinical and biological datasets collected in the digital platform developed and managed by Istituto Superiore di Sanità Using the ML approach a subset of artificial intelligence the University Federico II of Naples will analyze the dataset to establish relationships without preassigned modeling ML is an innovative approach to provide robust predictions overcoming descriptive statistical models and proving a digital twin for the considered phenomena Clinical behavioral inflammatory and immunological factors are altered in ASD However the great variability among individuals with ASD of these problems makes it difficult to predict who is most likely to respond to a specific intervention at what intensity and for what duration To this aim the investigators will develop a probabilistic multi-domain data integration model consisting of the gut microbiome immune and inflammatory biomarkers in peripheral blood and fecal samples and clinicalbehavioral biomarkers using ML to improve therapeutics and predict the prognosis of ASD Clinical and behavioral variables to be included in the dataset will be collected through the testsquestionnaires described in the experimental design Gut microbiome immune and inflammatory variables will be gathered on blood and fecal samples at baseline Timepoint 0 and at the end of supplementation Timepoint 1 The composition of the gut microbiome will be characterized by shotgun metagenomics Genomes of dominant strains will also be reconstructed with a recently validated standard pipeline and compared to define if a strain-level variation exists upon treatment with butyrate Fecal Short-Chain Fatty Acids SCFAs acetate propionate and butyrate levels will be determined by gas chromatograph-mass spectrometry as previously described Altered composition of the gut microbiome may alter the gut barrier potentially allowing the translocation of bacteria and their antigens toxins and metabolites and result in multiple alterations of cytokines production immune cell populations as well as cellular activation markers
Randomization and allocation concealment will be performed by the Istituto Superiore di Sanità Randomization will be generated using the procedure Ralloc in STATA managed through the RECap platform after enrollment and provided to clinicians by the Istituto Superiore di Sanità Specific standard operative procedures SOPs were defined to garantee study and trial integrity A blinded statistician will analyze data using Stata 161
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None