Ignite Creation Date:
2024-10-25 @ 7:48 PM
Last Modification Date:
2024-10-26 @ 3:34 PM
Study NCT ID:
NCT06502210
Status:
RECRUITING
Last Update Posted:
None
First Post:
2024-06-30
Brief Title:
Identification of New Gene Spliceosomes in Neuroblastoma
Sponsor:
None
Organization:
None
Study Overview
Official Title:
Identification of New Gene Spliceosomes in Neuroblastoma and Their Translational Application in Clinical Accurate Diagnosis and Therapy
Status:
RECRUITING
Status Verified Date:
2024-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Neuroblastoma is an early childhood embryonic malignancy that originates from neural crest cells Neuroblastoma shows high heterogeneity in biological morphological genetic and clinical features At present the main treatment methods for neuroblastoma are surgical treatment combined with chemotherapy after the operation and immunological therapy However clinical studies have found that 4050 of patients dont have good outcomes after postoperative chemotherapy
The clinical trial study aims to screen the genotype of children with neuroblastoma and conduct an in-depth analysis of sequencing data and tumor-specific transcripts by using technologies--Deep Sequencing Technology and Third Generation Sequencing Technology
The main questions this study aims to answer are Question 1This study will use Third Generation Sequencing technology to find specific transcript variants associated with cancer differentiation Question 2This study will identify possible tissue differential expression by using the Deep Sequencing Technology
Participants will undergo surgery during which doctors will remove tumor tissue and adjacent normal tissue In this experiment the tumor tissue of children with neuroblastoma will be used as the experimental group and the adjacent normal tissue will be used as the control group In this study the different genotypes of children with neuroblastoma are screened by Deep Sequencing Technology and Third Generation Sequencing Technology And according to the difference in genotypes doctors will treat children with neuroblastoma personally This study hopes to find new single nucleotide polymorphism and therapeutic targets
The clinical trial study aims to screen the genotype of children with neuroblastoma and conduct an in-depth analysis of sequencing data and tumor-specific transcripts by using technologies--Deep Sequencing Technology and Third Generation Sequencing Technology
The main questions this study aims to answer are Question 1This study will use Third Generation Sequencing technology to find specific transcript variants associated with cancer differentiation Question 2This study will identify possible tissue differential expression by using the Deep Sequencing Technology
Participants will undergo surgery during which doctors will remove tumor tissue and adjacent normal tissue In this experiment the tumor tissue of children with neuroblastoma will be used as the experimental group and the adjacent normal tissue will be used as the control group In this study the different genotypes of children with neuroblastoma are screened by Deep Sequencing Technology and Third Generation Sequencing Technology And according to the difference in genotypes doctors will treat children with neuroblastoma personally This study hopes to find new single nucleotide polymorphism and therapeutic targets
Detailed Description:
The clinical trial study aims to screen the genotype of children with neuroblastoma and conduct an in-depth analysis of sequencing data and tumor-specific transcripts using technologies--Deep Sequencing Technology and Third Generation Sequencing Technology This study hopes to find new single nucleotide polymorphism and therapeutic targets
Firstly researchers collect tumor tissues and adjacent normal tissues of 20 children with neuroblastoma meeting the inclusion criteria and collect their clinical data In this experiment the tumor tissue of children with neuroblastoma will be used as the experimental group and the adjacent normal tissue will be used as the control group
Secondly researchers will find differentially expressed genes using Deep Sequencing technology and Third Generation Sequencing Technology to sequence 7 neuroblastoma cell lines The expression of the transcription isoforms was divided into one group of N-myc gene amplification and another group of N-myc gene non-amplification by using Third Generation Sequencing Technology After extracting the total RNA from tissues researchers check the RNAs purity concentration and integrity If the RNA is tested up to standard researchers will build a gene library After the construction of the gene library is completed researchers will use the machine to sequence tissues
Finally survival analysis and repeated measurements will be used to analyze the data of the experimental group and control group in this experiment T-test will be used for measurement data χ2 test will be used for counting data Kaplan-Meier method and Cox regression analysis will be used for survival analysis All statistical analyses will be performed using Statistical Analysis Software Stata 260 P 005 is considered significant
Firstly researchers collect tumor tissues and adjacent normal tissues of 20 children with neuroblastoma meeting the inclusion criteria and collect their clinical data In this experiment the tumor tissue of children with neuroblastoma will be used as the experimental group and the adjacent normal tissue will be used as the control group
Secondly researchers will find differentially expressed genes using Deep Sequencing technology and Third Generation Sequencing Technology to sequence 7 neuroblastoma cell lines The expression of the transcription isoforms was divided into one group of N-myc gene amplification and another group of N-myc gene non-amplification by using Third Generation Sequencing Technology After extracting the total RNA from tissues researchers check the RNAs purity concentration and integrity If the RNA is tested up to standard researchers will build a gene library After the construction of the gene library is completed researchers will use the machine to sequence tissues
Finally survival analysis and repeated measurements will be used to analyze the data of the experimental group and control group in this experiment T-test will be used for measurement data χ2 test will be used for counting data Kaplan-Meier method and Cox regression analysis will be used for survival analysis All statistical analyses will be performed using Statistical Analysis Software Stata 260 P 005 is considered significant
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None