Ignite Creation Date:
2024-07-17 @ 11:52 AM
Last Modification Date:
2024-10-26 @ 3:33 PM
Study NCT ID:
NCT06475651
Status:
NOT_YET_RECRUITING
Last Update Posted:
2024-06-26
First Post:
2024-06-20
Brief Title:
Characterization and Contribution of Genome-wide DNA Methylation DNA Methylation Episignatures in Rare Diseases With Prenatal Onset
Sponsor:
Assistance Publique - Hôpitaux de Paris
Organization:
Assistance Publique - Hôpitaux de Paris
Study Overview
Official Title:
Characterization and Contribution of Genome-wide DNA Methylation DNA Methylation Episignatures in Rare Diseases With Prenatal Onset
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-06
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
FOETEPISIGN
Brief Summary:
It is necessary to define reference DNA Methylation Episignatures from fetal DNA The hypotheses are
It is possible to define reference DNA Methylation Episignatures from fetal DNA extracted from amniotic fluid or frozen tissues collected during the postmortem examination
Fetal DNA Methylation Episignatures may be different to postanal DNA Methylation Episignatures defined on DNA extracted from blood
It is possible to define reference DNA Methylation Episignatures from fetal DNA extracted from amniotic fluid or frozen tissues collected during the postmortem examination
Fetal DNA Methylation Episignatures may be different to postanal DNA Methylation Episignatures defined on DNA extracted from blood
Detailed Description:
Congenital anomalies CA complicate 3 to 5 of pregnancies and may be associated with genetic disorders Diagnosis of genetic diseases is a major medical challenge especially during pregnancy
Over the past two decades next-generation sequencing NGS has revolutionized our ability to identify the genetic condition associated with CA During pregnancy prenatal exome sequencing identified an additional diagnosis in around 30 of fetuses with CA when standard chromosomal investigations karyotype and chromosomal microarray analysis CMA fail to provide a diagnosis
Despite these major advances around 40 of rare diseases remain unsolved including 10-15 of patients harboring variants of uncertain significance VUS
After birth additional functional analyses multi-OMICS including genome-wide DNA methylation studies may be offered to reclassify VUS
DNA methylation anomalies play an important role in pathologies developmental disorders and oncology
DNA methylation Episignatures defined as the cumulative DNA methylation patterns occurring at multiple CpG dinucleotides across the genome have been recognized to be intricately associated with many human traits including age sex and disease status Recently DNA Methylation Episignatures have been identified in the blood of children or adults for several well-characterized genetic diseases However these postnatal DNA Methylation Episignatures cannot be used during pregnancy because DNA methylation changes from one tissue to another and during time especially during fetal developpement In addition the tissues available during pregnancy are different from those analyzed postnatally blood
Over the past two decades next-generation sequencing NGS has revolutionized our ability to identify the genetic condition associated with CA During pregnancy prenatal exome sequencing identified an additional diagnosis in around 30 of fetuses with CA when standard chromosomal investigations karyotype and chromosomal microarray analysis CMA fail to provide a diagnosis
Despite these major advances around 40 of rare diseases remain unsolved including 10-15 of patients harboring variants of uncertain significance VUS
After birth additional functional analyses multi-OMICS including genome-wide DNA methylation studies may be offered to reclassify VUS
DNA methylation anomalies play an important role in pathologies developmental disorders and oncology
DNA methylation Episignatures defined as the cumulative DNA methylation patterns occurring at multiple CpG dinucleotides across the genome have been recognized to be intricately associated with many human traits including age sex and disease status Recently DNA Methylation Episignatures have been identified in the blood of children or adults for several well-characterized genetic diseases However these postnatal DNA Methylation Episignatures cannot be used during pregnancy because DNA methylation changes from one tissue to another and during time especially during fetal developpement In addition the tissues available during pregnancy are different from those analyzed postnatally blood
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None