Ignite Creation Date:
2024-07-17 @ 11:40 AM
Last Modification Date:
2024-10-26 @ 3:34 PM
Study NCT ID:
NCT06497608
Status:
NOT_YET_RECRUITING
Last Update Posted:
2024-07-12
First Post:
2024-07-04
Brief Title:
Single-Cell Insights Into Diabetic Retinopathy Mechanisms
Sponsor:
Shanghai General Hospital Shanghai Jiao Tong University School of Medicine
Organization:
Shanghai General Hospital Shanghai Jiao Tong University School of Medicine
Study Overview
Official Title:
Exploring the Cellular and Molecular Mechanisms of the Development and Progression of Diabetic Retinopathy Based on Single-cell Sequencing
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
This study aims to uncover the cellular and molecular mechanisms of diabetic retinopathy through single-cell sequencing to identify new therapeutic targets
The study will collect retinal tissue samples from 3 patients with advanced proliferative diabetic retinopathy who underwent prosthetic eye implantation and 3 normal cadaver donors Additionally aqueous humor vitreous fluid and plasma samples from patients with severe diabetic retinopathy requiring surgery and macular hole surgery patients without diabetic retinopathy will be analyzed using ELISA qPCR and Western Blot These experiments will validate sequencing results and explore the pathogenesis of diabetic retinopathy to identify potential treatment targets
The study will collect retinal tissue samples from 3 patients with advanced proliferative diabetic retinopathy who underwent prosthetic eye implantation and 3 normal cadaver donors Additionally aqueous humor vitreous fluid and plasma samples from patients with severe diabetic retinopathy requiring surgery and macular hole surgery patients without diabetic retinopathy will be analyzed using ELISA qPCR and Western Blot These experiments will validate sequencing results and explore the pathogenesis of diabetic retinopathy to identify potential treatment targets
Detailed Description:
Diabetic retinopathy is the leading cause of blindness among the working population worldwide However its pathogenesis is not fully understood and effective prevention and treatment methods are limited This study initiated by Shanghai First Peoples Hospital aims to explore the cellular and molecular mechanisms underlying its occurrence through methods such as single-cell sequencing to identify new intervention targets This study plans to collect retinal tissue samples from 3 patients each who underwent prosthetic eye implantation due to late-stage proliferative diabetic retinopathy blindness and from 3 normal cadaver donors at the Ophthalmology Department of Shanghai First Peoples Hospital for single-cell sequencing and bioinformatics analysis Additionally samples of aqueous humor vitreous fluid and plasma will be collected from 30 patients with severe diabetic retinopathy requiring surgery and from 30 macular hole surgery patients without diabetic retinopathy These samples will be subjected to experiments such as ELISA qPCR and Western Blot to validate sequencing results investigate the pathogenesis of diabetic retinopathy and identify potential therapeutic targets
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None