Ignite Creation Date:
2024-06-16 @ 11:49 AM
Last Modification Date:
2024-10-26 @ 3:30 PM
Study NCT ID:
NCT06432556
Status:
NOT_YET_RECRUITING
Last Update Posted:
2024-05-29
First Post:
2024-04-29
Brief Title:
Study of Cellular Heterogeneity in Patients With Mastocytosis
Sponsor:
University Hospital Toulouse
Organization:
University Hospital Toulouse
Study Overview
Official Title:
Study of Cellular Heterogeneity in Patients With Mastocytosis MastCellHet Mastocytosis Cell Heterogeneity
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
MastCellHet
Brief Summary:
This study will aim to study the heterogeneity of skin-resident mast cells and of blood circulating hematopoietic progenitors in patients suffering from isolated Cutaneous Mastocytosis and from systemic Mastocytosis with skin lesions
Detailed Description:
Mastocytosis is a rare disease caused by abnormal mast cell accumulationproliferation Its clinical features are very heterogeneous Among adult patients 15 present an isolated cutaneous mastocytosis CM while 85 of them present a systemic mastocytosis SM with cutaneous lesions In addition regardless of the diagnosis ie CM or SM it is frequent to observe mast cell-dependent symptoms of variable severity ranging from gastrointestinal discomfort to life-threatening reactions To date the origin of such heterogeneous manifestations in adult patients is still elusive Researchers hypothesize that the heterogeneity in mastocytosis symptoms might originate at least in part from a broad diversity of mast cell populations in patients This study will aim to uncover heterogeneity of skin-resident mast cells and of blood circulating hematopoietic progenitors in patients suffering from isolated CM or SM with skin lesions Patients with isolated CM and patients with SM with cutaneous involvement will be recruited from the Mastocytosis Expert Center of Toulouse
Cluster of Differentiation CD 45 cells from skin biopsies and CD 34 cells from blood will be isolated by magnetic cell sorting for scRNAseq Bioinformatics analysis pipeline will be used in order to analyze the cellular heterogeneity of skin lesions and blood from CM and SM patients by comparing their transcriptomic signatures Using trajectories analysis the researchers will then deduce infer a differentiation pathway between blood progenitors and cutaneous mast cells at the patient level Researchers will then confirm the expression of identified relevant biomarkers by highly multiplexed imaging in frozen skin biopsies from CM patients and from SM patients
Cluster of Differentiation CD 45 cells from skin biopsies and CD 34 cells from blood will be isolated by magnetic cell sorting for scRNAseq Bioinformatics analysis pipeline will be used in order to analyze the cellular heterogeneity of skin lesions and blood from CM and SM patients by comparing their transcriptomic signatures Using trajectories analysis the researchers will then deduce infer a differentiation pathway between blood progenitors and cutaneous mast cells at the patient level Researchers will then confirm the expression of identified relevant biomarkers by highly multiplexed imaging in frozen skin biopsies from CM patients and from SM patients
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 2024-A00021-46 | OTHER | ID-RCB | None |