Ignite Creation Date:
2024-06-16 @ 11:49 AM
Last Modification Date:
2024-10-26 @ 3:30 PM
Study NCT ID:
NCT06432855
Status:
NOT_YET_RECRUITING
Last Update Posted:
2024-06-24
First Post:
2024-05-22
Brief Title:
Genetic Determinants of the Antiviral Immune Response in Oceanian Populations
Sponsor:
Institut Pasteur
Organization:
Institut Pasteur
Study Overview
Official Title:
Genetic Determinants of the Antiviral Immune Response in Oceanian Populations
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
GEDIPOP
Brief Summary:
Oceanias populations including Melanesians are paying a heavy price for dengue fever which has been circulating actively in the region since the Second World War In New Caledonia NC the incidence of dengue fever is higher among municipalities predominantly populated by Melanesians suggesting that Melanesians may have an increased susceptibility to symptomatic dengue fever Differences in antiviral immune responses between populations of different geographical origins are partly the result of population-specific immune regulatory variants In turn viruses have imposed considerable selective pressure on human populations Although crucial to understanding their susceptibility to viral infections the genetic determinants of the antiviral immune response of Oceanians remain to be characterized
In this context the hypothesis is that the genetic origin of Oceanians and Melanesians in particular has shaped their antiviral immune response and contributes to their greater susceptibility to certain viral infections The aim is to characterize the immune response to pathogens affecting the New Caledonian population and in particular to dengue virus of Melanesian and European populations and to identify its genetic determinants It will be explored whether saliva can be used as a non-invasive sample to study the seroprevalence of dengue in Oceanian populations
In this context the hypothesis is that the genetic origin of Oceanians and Melanesians in particular has shaped their antiviral immune response and contributes to their greater susceptibility to certain viral infections The aim is to characterize the immune response to pathogens affecting the New Caledonian population and in particular to dengue virus of Melanesian and European populations and to identify its genetic determinants It will be explored whether saliva can be used as a non-invasive sample to study the seroprevalence of dengue in Oceanian populations
Detailed Description:
Cross-sectional observational study with prospective sample and data collection Individuals will be identified from among eligible participants in the STEP-BSA21 and COVCAL studies
Information and consent Questionnaire saliva collection on Oragene tube buccal swabbing and 20 mL blood collection on CPT tube
DNA extraction and low-coverage sequencing of participants complete genomes And Isolation and in vitro stimulation of peripheral blood mononuclear cells PBMCs with dengue virus
single-cell RNA sequencing scRNAseq
multiplex quantification of cytokines and chemokines
bioinformatics analysis to identify genetic markers associated with a differential transcriptomic and secretory response to dengue virus stimulation
Information and consent Questionnaire saliva collection on Oragene tube buccal swabbing and 20 mL blood collection on CPT tube
DNA extraction and low-coverage sequencing of participants complete genomes And Isolation and in vitro stimulation of peripheral blood mononuclear cells PBMCs with dengue virus
single-cell RNA sequencing scRNAseq
multiplex quantification of cytokines and chemokines
bioinformatics analysis to identify genetic markers associated with a differential transcriptomic and secretory response to dengue virus stimulation
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 2023-A02206-39 | OTHER | ID RCB | None |