Ignite Creation Date:
2024-05-06 @ 8:23 PM
Last Modification Date:
2024-10-26 @ 3:27 PM
Study NCT ID:
NCT06367413
Status:
RECRUITING
Last Update Posted:
2024-06-07
First Post:
2024-04-07
Brief Title:
Effects of Protein and NAC Co-ingestion on Skeletal Muscle Recovery
Sponsor:
University of Thessaly
Organization:
University of Thessaly
Study Overview
Official Title:
Effects of Whey Protein and N-acetylcysteine Co-ingestion on Skeletal Muscle Recovery Following Exercise-induced Muscle Damage
Status:
RECRUITING
Status Verified Date:
2024-06
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
PRoNAC
Brief Summary:
Previous evidence suggests that though N-acetylcysteine NAC supplementation following eccentric exercise-induced muscle damage disrupts the skeletal muscles repair and remodelling process at 8 days of recovery it attenuates substantially the decline of skeletal muscle performance during the first 48 hours of recovery The enhanced performance capacity during the first phase of recovery in response to NAC supplementation might be attributed to the altered redox status in skeletal muscle as a consequence of the NAC-mediated elevation of reduced glutathione GSH levels The rise in GSH results in a redox-dependent attenuation of immune cell mobilisation and reduction of oxidative stress response leading to a blunted rise of muscle damage and inflammatory markers during the first 2-3 days of recovery However following exercise-induced muscle damage protein synthesis increases in skeletal muscle over the next 24-48 hours to support its repair process and thus protein supplementation might accelerate the recovery process by enhancing the protein synthetic response Therefore the present study aims at investigating for first time the combined effect of NAC and whey protein supplementation on the short-term during the first 72 hours recovery process of skeletal muscle following damaging exercise eccentric exercise and compared it with the well-documented efficacy of NAC supplementation The results of this study might be particularly useful for athletes such as those in soccer and basketball that participate in 3 games or intense training sessions during the same macrocycle with only 48-72 hours of recovery in-between
Detailed Description:
Participants will initially undergo baseline testing including assessment of their i anthropometrics ii body composition via DXA iii habitual physical activity level via accelerometry iv resting metabolic rate via indirect calorimetry v daily dietary intake via 7-day dietary recalls and vi cardiorespiratory fitness using a treadmill ramp test After baseline testing participants will be assigned to a a Placebo b a N-acetylcysteine NAC or c a N-acetylcysteine Whey protein PRoNAC group in a randomized double-blind placebo-controlled parallel group repeated measures design In all groups participants will execute an eccentric exercise protocol on an isokinetic dynamometer consisted of 150 eccentric muscle contractions Before exercise as well as at 1- 2- 3- 24- 48- and 72-hours post-exercise they will undergo assessment of their maximal voluntary isometric peal torque maximal countermovement jump height and muscle soreness level In addition blood sample will be drawn before exercise and at 24- 48- and 72-hours post-exercise for the determination of myoglobin concentration reduced GSH and oxidized GSSG glutathione content protein carbonyl formation and catalase activity
Throughout the 4-day experimental period Day 1 exercise day Days 2 3 and 4 24- 48- and 72-hours post-exercise respectively participants will receive daily 3 doses from the respective supplement as follows the 1st dose at mid-morning between breakfast and lunch the 2nd dose at mid-afternoon post-exercise on day 1 and between lunch and dinner on days 2-4 and the 3rd dose before sleep In Placebo group participants will be supplemented with 031 gr of maltodextrin per kg of body weight in the first two servings at mid-morning and mid-afternoon supplements and with 049 gr of maltodextrin per kg of body weight in the pre-sleep serving 11 gr maltodextrinkg BWday In NAC group participants will be supplemented with 1333 mg of N-acetylcysteine per kg of body weight per serving 40 mg NACkg BWday In PRoNAC group participants will be supplemented with 031 gr of whey protein and 1333 mg of N-acetylcysteine per kg of body weight in the first two servings at mid-morning and mid-afternoon supplements and with 049 gr of whey protein and 1333 mg of N-acetylcysteine per kg of body weight in the pre-sleep serving 11 gr Whey protein 40 mg NAC kg BWday
Throughout the 4-day experimental period Day 1 exercise day Days 2 3 and 4 24- 48- and 72-hours post-exercise respectively participants will receive daily 3 doses from the respective supplement as follows the 1st dose at mid-morning between breakfast and lunch the 2nd dose at mid-afternoon post-exercise on day 1 and between lunch and dinner on days 2-4 and the 3rd dose before sleep In Placebo group participants will be supplemented with 031 gr of maltodextrin per kg of body weight in the first two servings at mid-morning and mid-afternoon supplements and with 049 gr of maltodextrin per kg of body weight in the pre-sleep serving 11 gr maltodextrinkg BWday In NAC group participants will be supplemented with 1333 mg of N-acetylcysteine per kg of body weight per serving 40 mg NACkg BWday In PRoNAC group participants will be supplemented with 031 gr of whey protein and 1333 mg of N-acetylcysteine per kg of body weight in the first two servings at mid-morning and mid-afternoon supplements and with 049 gr of whey protein and 1333 mg of N-acetylcysteine per kg of body weight in the pre-sleep serving 11 gr Whey protein 40 mg NAC kg BWday
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
False
Is an FDA AA801 Violation?:
None