Ignite Creation Date:
2024-05-06 @ 8:23 PM
Last Modification Date:
2024-10-26 @ 3:26 PM
Study NCT ID:
NCT06365320
Status:
NOT_YET_RECRUITING
Last Update Posted:
2024-04-15
First Post:
2024-03-25
Brief Title:
Association Between Training Load and Lactate and Other Metabolites Analyzed by Metabolomic and Proteomic Techniques
Sponsor:
Fundació Eurecat
Organization:
Fundació Eurecat
Study Overview
Official Title:
Evaluation of the Relationship Between Training Load and the Levels of Lactate and Other Metabolites Analyzed in Blood and Saliva Samples Using Metabolomic and Proteomic Techniques in Federated Basketball Players Quasi-experimental Study
Status:
NOT_YET_RECRUITING
Status Verified Date:
2024-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
PROTEOMET
Brief Summary:
Physical exercise induces numerous changes in the body in a complex signalling network caused by or in response to increased metabolic activity of contracting skeletal muscles
The application of omics analytical techniques such as proteomics and metabolomics in the field of sport allows us to understand how the human body responds to exercise and how sports results can be improved by optimising nutrition and training Both omics techniques offer a quantitative measurement of the metabolic profiles associated with exercise and are able to identify metabolic signatures of athletes from different sports disciplines
Basketball is a high-intensity exercise modality interspersed with low-intensity The performance requirements of basketball include aerobic and anaerobic metabolism with anaerobic metabolism being considered the main energy system Therefore basketball players need great athletic ability to produce a successful performance during competition
For optimal sports performance it is important to adjust the training load ie the degree of effort that the player can withstand in a single training session Coaches require effective and objective load monitoring tools that allow them to make decisions about training plans based on the needs of each player
Microsampling systems emerge as an alternative to venipuncture by facilitating self-sampling which can be carried out outside healthcare centres in a comfortable and precise way from a small finger prick that the user can perform These systems are less expensive and can be effective in measuring the levels of glucose metabolism products such as lactate through the application of metabolomics and proteomics On the other hand the use of non-invasive methods of measuring lactate levels is becoming increasingly popular in sports medicine The use of saliva as an alternative fluid to the blood shows promise for identifying the concentrations of metabolites that occur during and after sports training
The application of omics analytical techniques such as proteomics and metabolomics in the field of sport allows us to understand how the human body responds to exercise and how sports results can be improved by optimising nutrition and training Both omics techniques offer a quantitative measurement of the metabolic profiles associated with exercise and are able to identify metabolic signatures of athletes from different sports disciplines
Basketball is a high-intensity exercise modality interspersed with low-intensity The performance requirements of basketball include aerobic and anaerobic metabolism with anaerobic metabolism being considered the main energy system Therefore basketball players need great athletic ability to produce a successful performance during competition
For optimal sports performance it is important to adjust the training load ie the degree of effort that the player can withstand in a single training session Coaches require effective and objective load monitoring tools that allow them to make decisions about training plans based on the needs of each player
Microsampling systems emerge as an alternative to venipuncture by facilitating self-sampling which can be carried out outside healthcare centres in a comfortable and precise way from a small finger prick that the user can perform These systems are less expensive and can be effective in measuring the levels of glucose metabolism products such as lactate through the application of metabolomics and proteomics On the other hand the use of non-invasive methods of measuring lactate levels is becoming increasingly popular in sports medicine The use of saliva as an alternative fluid to the blood shows promise for identifying the concentrations of metabolites that occur during and after sports training
Detailed Description:
The study hypothesizes that the use of minimally invasive microsampling systems and subsequent application of metabolomics and proteomics will allow the detection of differences in the levels of lactate and other metabolites and proteins produced by the greater energy demand of the musculoskeletal system after a single collective training on the court in federated basketball players In addition lactate levels will be correlated with the subjective sensation of perceived exertion
The main objective of the study is to apply metabolomics techniques to analyze lactate levels in capillary blood samples collected by a dried blood spot DBS microsampling device and to study their correlation with the subjective sensation of perceived effort in federated basketball players before and after performing a single collective training session on the court
The secondary objectives of the study are to measure the change in lactate levels in capillary blood samples collected by a DBS device and in saliva samples collected by a collector before and after performing a single collective training on the court In addition in these samples the change in the levels of other metabolomic and proteomic markers related to energy lipid and amino acid metabolism will be measured The correlation between salivary and blood lactate levels will also be studied subjective sensation of perceived exertion and salivary lactate levels the subjective sensation of perceived exertion and the levels of other metabolomic and proteomic markers will be also studied
A single-group quasi-experimental or pre-post study will be carried out on 70 basketball players between the ages of 18 and 40
Each participant will attend 2 visits to the sports facilities of their basketball club
A recruitment and pre-selection visit to check the eligibility criteria and sign the informed consent If the inclusion criteria are met will be scheduled to
A single study visit on the same day agreed for training
The main variable of the study is the correlation between lactate levels measured in capillary blood pre- and post-training and the subjective sensation of perceived effort
The main objective of the study is to apply metabolomics techniques to analyze lactate levels in capillary blood samples collected by a dried blood spot DBS microsampling device and to study their correlation with the subjective sensation of perceived effort in federated basketball players before and after performing a single collective training session on the court
The secondary objectives of the study are to measure the change in lactate levels in capillary blood samples collected by a DBS device and in saliva samples collected by a collector before and after performing a single collective training on the court In addition in these samples the change in the levels of other metabolomic and proteomic markers related to energy lipid and amino acid metabolism will be measured The correlation between salivary and blood lactate levels will also be studied subjective sensation of perceived exertion and salivary lactate levels the subjective sensation of perceived exertion and the levels of other metabolomic and proteomic markers will be also studied
A single-group quasi-experimental or pre-post study will be carried out on 70 basketball players between the ages of 18 and 40
Each participant will attend 2 visits to the sports facilities of their basketball club
A recruitment and pre-selection visit to check the eligibility criteria and sign the informed consent If the inclusion criteria are met will be scheduled to
A single study visit on the same day agreed for training
The main variable of the study is the correlation between lactate levels measured in capillary blood pre- and post-training and the subjective sensation of perceived effort
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None