Ignite Creation Date:
2024-05-06 @ 8:08 PM
Last Modification Date:
2024-10-26 @ 3:21 PM
Study NCT ID:
NCT06265519
Status:
RECRUITING
Last Update Posted:
2024-02-21
First Post:
2024-02-02
Brief Title:
Association of Urine BDNF and NGF With Lower Urinary System Parameters
Sponsor:
Marmara University
Organization:
Marmara University
Study Overview
Official Title:
The Association of Urinary BDNF and NGF With Lower Urinary System Parameters in Patients With Bladder Outlet Obstruction Secondary to Benign Prostate Hyperplasia
Status:
RECRUITING
Status Verified Date:
2024-02
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Histologically BPH is a benign proliferative process involving both epithelial and stromal elements and is characterised by progressive enlargement of the prostate Symptom complex including increased frequency of urination sudden feeling of urge to urinate nocturia difficulty in urinating feeling of incomplete emptying of the bladder decreased flow rate and intermittent urination are called lower urinary tract symptoms LUTS The most important cause of LUTS in men is BPH Many structural and physiological changes occur in the lower urinary system with bladder outlet obstruction Detrusor hypertrophy and bladder hyperactivity may occur due to bladder outlet obstruction Although the density of afferent and efferent nerves in the bladder decreases after urethral obstruction enlargement of their trunks indicates that changes occur in these nerves In addition changes also occur in the neural pathways of the central nervous system following lower urinary tract obstruction Nerve growth factor NGF and brain derived neurotropin factor BDNF are trophic proteins that act as retrograde messengers between peripheral effector tissue and the nerves that innervate it In peripheral tissues the source of NGF and BDNF is presumed to be the target tissues innervated by nerves Smooth muscle cells fibroblasts astrocytes and other cells synthesise NGF and BDNF in culture medium Many potential stimuli that increase NGF in the lower urinary system have been identified These are denervation inflammation and mechanical tension This information has led to the idea that autonomic innervation changes in the bladder may be related with changing NGF levels Altered afferent and adrenergic innervation in the obstructed bladder increases the possibility that NGF plays an important role in this neural growth because this type of nerves are highly sensitive to this neurotrophin
In this study we investigated NGF ve BDNF levels in urine samples obtained before surgery Transurethral Prostate Resection Prostate Enucleation with Holmium Laser and Prostate Enucleation with Thulium Fibre Laser and after removal of obstruction in patients with bladder outlet obstruction secondary to benign prostatic enlargement using ELISA method We aimed to determine the role of NGF and BDNF in bladder outlet obstruction and bladder changes secondary to obstruction by comparing with control patients without obstruction
In this study we investigated NGF ve BDNF levels in urine samples obtained before surgery Transurethral Prostate Resection Prostate Enucleation with Holmium Laser and Prostate Enucleation with Thulium Fibre Laser and after removal of obstruction in patients with bladder outlet obstruction secondary to benign prostatic enlargement using ELISA method We aimed to determine the role of NGF and BDNF in bladder outlet obstruction and bladder changes secondary to obstruction by comparing with control patients without obstruction
Detailed Description:
As the demographic structure of societies changes benign prostatic hyperplasia BPH is one of the most important health problems for older men especially in developed countries Histologically BPH is a benign proliferative process involving both epithelial and stromal elements and is characterised by progressive enlargement of the prostate BPH is a lifelong chronic disease with an incidence of approximately 8 in men aged 31-40 years which increases rapidly with age and reaches 90 in the 9th decade Symptom complex including increased frequency of urination sudden feeling of urge to urinate nocturia difficulty in urinating feeling of incomplete emptying of the bladder decreased flow rate and intermittent urination are called lower urinary tract symptoms LUTS The most important cause of LUTS in men is BPH Many structural and physiological changes occur in the lower urinary system with bladder outlet obstruction Detrusor hypertrophy and bladder hyperactivity may occur due to bladder outlet obstruction Although the density of afferent and efferent nerves in the bladder decreases after urethral obstruction enlargement of their trunks indicates that changes occur in these nerves
In addition changes also occur in the neural pathways of the central nervous system following lower urinary tract obstruction Nerve growth factor NGF and brain derived neurotropin factor BDNF are trophic proteins that act as retrograde messengers between peripheral effector tissue and the nerves that innervate it In peripheral tissues the source of NGF and BDNF is presumed to be the target tissues innervated by nerves Smooth muscle cells fibroblasts astrocytes and other cells synthesise NGF and BDNF in culture medium NGF is required for survival development and neurotransmitter synthesis regulation of dorsal root ganglion and sympathetic cells in embryonic and postnatal life NGF receptor contains two subunits the low affinity subunit is called p75 and the high affinity tyrosine kinase subunit is called tyrosine kinase A which is responsible for the growth and survival effects of NGF Many potential stimuli that increase NGF in the lower urinary system have been identified These are denervation inflammation and mechanical tension This information has led to the idea that autonomic innervation changes in the bladder may be related with changing NGF levels Altered afferent and adrenergic innervation in the obstructed bladder increases the possibility that NGF plays an important role in this neural growth because this type of nerves are highly sensitive to this neurotrophin Clinical and experimental data have shown a relationship between urinary NGF and overactive bladder Overactive bladder OAB is a complex of uncomfortable symptoms accompanied by a feeling of urgency frequent urination and nocturia with or without urge incontinence Overactive bladder is thought to occur as a result of an inflammatory process occurring in the bladder The reason for this is shown as high levels of inflammation mediators in bladder biopsies and urine of OAB patients NGF which is one of the inflammation mediators was found to be high in OAB patients in studies and it was observed that NGF level decreased after antimuscarinic treatment or botulinum neurotoxin injection
In this study we investigated NGF ve BDNF levels in urine samples obtained before surgery Transurethral Prostate Resection Prostate Enucleation with Holmium Laser and Prostate Enucleation with Thulium Fibre Laser and after removal of obstruction in patients with bladder outlet obstruction secondary to benign prostatic enlargement using ELISA method We aimed to determine the role of NGF and BDNF in bladder outlet obstruction and bladder changes secondary to obstruction by comparing with control patients without obstruction
In addition changes also occur in the neural pathways of the central nervous system following lower urinary tract obstruction Nerve growth factor NGF and brain derived neurotropin factor BDNF are trophic proteins that act as retrograde messengers between peripheral effector tissue and the nerves that innervate it In peripheral tissues the source of NGF and BDNF is presumed to be the target tissues innervated by nerves Smooth muscle cells fibroblasts astrocytes and other cells synthesise NGF and BDNF in culture medium NGF is required for survival development and neurotransmitter synthesis regulation of dorsal root ganglion and sympathetic cells in embryonic and postnatal life NGF receptor contains two subunits the low affinity subunit is called p75 and the high affinity tyrosine kinase subunit is called tyrosine kinase A which is responsible for the growth and survival effects of NGF Many potential stimuli that increase NGF in the lower urinary system have been identified These are denervation inflammation and mechanical tension This information has led to the idea that autonomic innervation changes in the bladder may be related with changing NGF levels Altered afferent and adrenergic innervation in the obstructed bladder increases the possibility that NGF plays an important role in this neural growth because this type of nerves are highly sensitive to this neurotrophin Clinical and experimental data have shown a relationship between urinary NGF and overactive bladder Overactive bladder OAB is a complex of uncomfortable symptoms accompanied by a feeling of urgency frequent urination and nocturia with or without urge incontinence Overactive bladder is thought to occur as a result of an inflammatory process occurring in the bladder The reason for this is shown as high levels of inflammation mediators in bladder biopsies and urine of OAB patients NGF which is one of the inflammation mediators was found to be high in OAB patients in studies and it was observed that NGF level decreased after antimuscarinic treatment or botulinum neurotoxin injection
In this study we investigated NGF ve BDNF levels in urine samples obtained before surgery Transurethral Prostate Resection Prostate Enucleation with Holmium Laser and Prostate Enucleation with Thulium Fibre Laser and after removal of obstruction in patients with bladder outlet obstruction secondary to benign prostatic enlargement using ELISA method We aimed to determine the role of NGF and BDNF in bladder outlet obstruction and bladder changes secondary to obstruction by comparing with control patients without obstruction
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None