Ignite Creation Date:
2024-05-06 @ 7:50 PM
Last Modification Date:
2024-10-26 @ 3:15 PM
Study NCT ID:
NCT06153641
Status:
ACTIVE_NOT_RECRUITING
Last Update Posted:
2024-02-29
First Post:
2023-11-20
Brief Title:
Cytokeratin 18 Non-invasive Biomarker for Rejection in Liver Transplant Patients
Sponsor:
St Louis University
Organization:
St Louis University
Study Overview
Official Title:
Cytokeratin 18 a Novel Non-invasive Biomarker for Rejection in Liver Transplant Patients
Status:
ACTIVE_NOT_RECRUITING
Status Verified Date:
2024-09
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Liver transplantation is a lifesaving procedure however there are chances that the body may reject the organ following liver transplantation and this remains a significant concern This rejection of the transplanted healthy liver tissue further adds to the patients illness and also increases the related costs of treatment Currently liver biopsy is the standard procedure used for diagnosing this rejection Being an invasive procedure requiring the introduction of instruments into the body this procedure also increases the chances of death of the patient Researchers are looking into the identification of testing methods that can act as a sign of this rejection without requiring the introduction of instruments into the body This type of testing could also allow for adjusting the doses of drugs given to the patient to decrease the chances of graft failure
A particular event that occurs during rejection in the body is the death of liver cells Thus tracking cell death using a blood test would be an important tool in assessing rejection CK-18 is a protein in the liver cells that is thought to be linked to the changes occurring as a result of cell death This study will be looking into a new idea of measuring CK-18 levels and compare them to an existing index to develop a reliable test for liver transplant rejection without introducing any instruments into the body
The purpose of this research study is to assess the history and collect blood samples to be tested for measuring CK-18 levels and assess certain other markers in the blood
A particular event that occurs during rejection in the body is the death of liver cells Thus tracking cell death using a blood test would be an important tool in assessing rejection CK-18 is a protein in the liver cells that is thought to be linked to the changes occurring as a result of cell death This study will be looking into a new idea of measuring CK-18 levels and compare them to an existing index to develop a reliable test for liver transplant rejection without introducing any instruments into the body
The purpose of this research study is to assess the history and collect blood samples to be tested for measuring CK-18 levels and assess certain other markers in the blood
Detailed Description:
Cellular rejection following liver transplantation is of worldwide occurrence and a major cause of morbidity affecting 15 to 20 percent of liver transplantation recipients Elevation of serum aminotransferase may be suspicious of rejection and typically precedes clinical symptoms of fever and jaundice However such clinical signs as well biochemical parameters are neither specific nor sensitive for detection of rejection or its degree of severity
The current standard of care remains a liver biopsy for confirmation of transplant rejection While significant procedural advances have been made liver biopsy is an invasive procedure requiring sedation and has a small but definite risk of complications including mortality Developing noninvasive biomarkers which could mitigate the need for an invasive liver biopsy remains a major national and international research focus in the field of hepatology This could also lay the foundation for early non-invasive longitudinal tracking of rejection and help in decreasing graft failure
Cytokeratin 18 is a cytoskeletal protein found in hepatocytes and is thought to be a major intermediate filament protein linked to the morphological changes of cell death During hepatocyte apoptosis cytokeratin 18 is cleaved by caspases The resulting novel neo-epitope occurs specifically during apoptosis and represents apoptotic cell death M30 a monoclonal antibody recognizes this cleaved epitope CK-18 and thus serves as a novel biomarker for apoptosis Apoptosis is a known event in rejection Several mechanisms have been postulated to lead to apoptosis during rejection which include granule-exocytosis pathway the Fas-mediated pathway and induction by cytokines Additionally there appears to be a significant co-relation between the grade of rejection and severity of apoptosis
These observations lead us into the exciting possibility that CK-18 measurements could prove to be a very valuable noninvasive biomarker for hepatic cellular rejection and could pave the path towards predictive models tracking rejection and its progression The purpose of this research study is to collect blood samples to be tested for measuring CK-18 levels assess certain other markers in the blood and assess the history
Approximately 105 patients will be enrolled in this study at Saint Louis University
This is a prospective study that includes three specific cohorts of subjects as follows no randomization of subjects
1 Post-transplant patients undergoing standard-of-care liver biopsy Biopsy arm
2 Stable post-liver transplantation patients without rejection Stable post-liver transplant arm
3 Healthy non-transplant control patients without liver disease Healthy arm
Each participant will be assigned a unique participant ID at the time of enrollment All participants data and samples collected will be saved by this unique participant ID All blood samples will be labeled with a study number and sent for assessment of CK-18 levels and other markers to understand rejection
A participant may spend less than one hour on this research study the time it takes to give consent review medical information and have their blood drawn
Reviewing their medical records will collect Information about participants age race gender and their health The research team will try to take the research blood sample when participants already have a blood test done for their regular health checks but there may be times when the participant must have blood drawn just for the study
The blood sample required for this research study will be collected as follows depending on the participant category may fall into
1 If the participant is a healthy patient or a participant who underwent liver transplantation and is stable post-transplantation with no symptoms and signs of rejection In that case the research sample will be collected with the standard of care lab work they will have as a part of routine healthcare If such is not possible the Participant may need to get an extra stick
2 If a participant is undergoing a liver biopsy In that case an intravenous line is usually placed as a standard of care at participating centers at the time of liver biopsy - especially in the pediatric population Research blood would be collected during line placement or with standard-of-care lab work
If such cannot be obtained then the research-only blood sample would be collected There may be times when the research team is unable to get adequate blood for our research study at this time which is when participants may need to have additional blood drawn for the study
Participants may be re-enrolled in the study more than once if they qualify Participants will be asked if they are willing to participate each time and will be re-consented each time
If a participant has a liver transplant after completing participation in the healthy arm the Participant may be eligible for re-enrollment in the study under other arms Stable post-liver transplant arm or biopsy arm
If a participant has an episode of rejection and is scheduled for liver biopsy after completing participation in the stable post-liver transplant arm the participant may be eligible for re-enrollment in the study under the biopsy arm since this study captures the rejection events
If a participant has a subsequent episode of rejection and is scheduled for another liver biopsy after completing participation in the biopsy arm the Participant may be eligible for re-enrollment in the study under the liver biopsy arm since this study captures the rejection events
If a participant get stable in the study doctors opinion after completing participation in the biopsy arm The participant may be eligible for re-enrollment in the study under the stable post-liver transplant arm
The current standard of care remains a liver biopsy for confirmation of transplant rejection While significant procedural advances have been made liver biopsy is an invasive procedure requiring sedation and has a small but definite risk of complications including mortality Developing noninvasive biomarkers which could mitigate the need for an invasive liver biopsy remains a major national and international research focus in the field of hepatology This could also lay the foundation for early non-invasive longitudinal tracking of rejection and help in decreasing graft failure
Cytokeratin 18 is a cytoskeletal protein found in hepatocytes and is thought to be a major intermediate filament protein linked to the morphological changes of cell death During hepatocyte apoptosis cytokeratin 18 is cleaved by caspases The resulting novel neo-epitope occurs specifically during apoptosis and represents apoptotic cell death M30 a monoclonal antibody recognizes this cleaved epitope CK-18 and thus serves as a novel biomarker for apoptosis Apoptosis is a known event in rejection Several mechanisms have been postulated to lead to apoptosis during rejection which include granule-exocytosis pathway the Fas-mediated pathway and induction by cytokines Additionally there appears to be a significant co-relation between the grade of rejection and severity of apoptosis
These observations lead us into the exciting possibility that CK-18 measurements could prove to be a very valuable noninvasive biomarker for hepatic cellular rejection and could pave the path towards predictive models tracking rejection and its progression The purpose of this research study is to collect blood samples to be tested for measuring CK-18 levels assess certain other markers in the blood and assess the history
Approximately 105 patients will be enrolled in this study at Saint Louis University
This is a prospective study that includes three specific cohorts of subjects as follows no randomization of subjects
1 Post-transplant patients undergoing standard-of-care liver biopsy Biopsy arm
2 Stable post-liver transplantation patients without rejection Stable post-liver transplant arm
3 Healthy non-transplant control patients without liver disease Healthy arm
Each participant will be assigned a unique participant ID at the time of enrollment All participants data and samples collected will be saved by this unique participant ID All blood samples will be labeled with a study number and sent for assessment of CK-18 levels and other markers to understand rejection
A participant may spend less than one hour on this research study the time it takes to give consent review medical information and have their blood drawn
Reviewing their medical records will collect Information about participants age race gender and their health The research team will try to take the research blood sample when participants already have a blood test done for their regular health checks but there may be times when the participant must have blood drawn just for the study
The blood sample required for this research study will be collected as follows depending on the participant category may fall into
1 If the participant is a healthy patient or a participant who underwent liver transplantation and is stable post-transplantation with no symptoms and signs of rejection In that case the research sample will be collected with the standard of care lab work they will have as a part of routine healthcare If such is not possible the Participant may need to get an extra stick
2 If a participant is undergoing a liver biopsy In that case an intravenous line is usually placed as a standard of care at participating centers at the time of liver biopsy - especially in the pediatric population Research blood would be collected during line placement or with standard-of-care lab work
If such cannot be obtained then the research-only blood sample would be collected There may be times when the research team is unable to get adequate blood for our research study at this time which is when participants may need to have additional blood drawn for the study
Participants may be re-enrolled in the study more than once if they qualify Participants will be asked if they are willing to participate each time and will be re-consented each time
If a participant has a liver transplant after completing participation in the healthy arm the Participant may be eligible for re-enrollment in the study under other arms Stable post-liver transplant arm or biopsy arm
If a participant has an episode of rejection and is scheduled for liver biopsy after completing participation in the stable post-liver transplant arm the participant may be eligible for re-enrollment in the study under the biopsy arm since this study captures the rejection events
If a participant has a subsequent episode of rejection and is scheduled for another liver biopsy after completing participation in the biopsy arm the Participant may be eligible for re-enrollment in the study under the liver biopsy arm since this study captures the rejection events
If a participant get stable in the study doctors opinion after completing participation in the biopsy arm The participant may be eligible for re-enrollment in the study under the stable post-liver transplant arm
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None