Ignite Creation Date:
2024-05-06 @ 7:42 PM
Last Modification Date:
2024-10-26 @ 3:12 PM
Study NCT ID:
NCT06105372
Status:
ENROLLING_BY_INVITATION
Last Update Posted:
2023-10-27
First Post:
2023-10-13
Brief Title:
Ramadan Fasting Microbiota and FABP4
Sponsor:
Okan University
Organization:
Okan University
Study Overview
Official Title:
Effects of Ramadan Fasting on the Intestinal Microbiota and Fatty Acid Binding Protein 4 FABP4 of Individuals
Status:
ENROLLING_BY_INVITATION
Status Verified Date:
2023-10
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
This study which aims to examine the effect of Ramadan fasting on intestinal microbiota composition and FABP4 was designed as a quasi-experimental study
This study is planned to be conducted with volunteer participants who fasted during the Ramadan and met the inclusion criteria
This study is planned to be conducted with volunteer participants who fasted during the Ramadan and met the inclusion criteria
Detailed Description:
The sample of the study was determined as 10 volunteer male participants who fasted during the Ramadan and met the inclusion criteria Participants will not be subjected to any energy restriction they will be fed ad-libitum and only the effect of intermittent fasting and fasting time on the variables will be examined
Intermittent fasting is supported by increasing studies showing that it has a purifying effect on health In particular fasting practices exceeding 16 hours induce autophagy at the cellular level and this continues its effect in all tissue and organ systems after the regeneration movement that starts at the cellular level
One of the important factors affected by intermittent fasting is the changes in microbiota which is closely related to the general health of humans Microbiota is generally defined as the colonization of certain places in our body by various populations of microorganisms and is affected by many factors such as nutrition lifestyle exercise drug use and stress
Fatty acid binding protein 4 FABP4 is an adipocyte member of the fatty acid binding protein FABP family with nine different isoforms It is highly secreted from adipose tissue but is also expressed from monocytes macrophages and dendritic cells This adipocyte which increases in obesity is found at higher levels in women than in men Studies have shown that increased serum FABP4 levels are associated with Type 1 and Type 2 diabetes mellitus DM insulin resistance and cardiovascular diseases
The aim of this thesis study was to investigate the effect of Ramadan fasting on gut microbiota and FABP4
The hypotheses of the study were
1 Gut microbiota composition of adult individuals differs before and after Ramadan fasting
2 Serum FABP4 and other biochemical values of adult individuals differ before and after Ramadan fasting
3 Anthropometric measurements of adults differ before and after Ramadan fasting All individuals who meet the inclusion and exclusion criteria and agree to participate in the study will be read and signed the Informed Voluntary Consent Form and a copy will be delivered to them Questions related to the socio-demographic status and dietary habits of all participants will be asked to them and recorded in the questionnaire form by the researcher using the face-to-face interview technique
Information about the volunteers who participated in the study such as not having any disease DM hypertension dyslipidemia cancer chronic renal failure GI problems etc not taking medication and not having used antibiotics in the last six months were evaluated by the Specialist Dr who took part in the study Dr evaluated the participants through a preliminary interview with the participants Factors such as the fact that the volunteers who participated in the study did not drink alcohol had not applied for any diet program in the last six months whether the participants were doing sahur or not and especially that all participants had the same duration of fasting during the day during Ramadan were evaluated by the researcher through a preliminary interview with the participants All individuals who met the inclusion and exclusion criteria and agreed to participate in the study were read and signed the Informed Voluntary Consent Form and a copy was delivered to them
In order to evaluate the nutritional status of the individuals the participants will be asked to record the amount of food consumption one week before the start of Ramadan fasting and during the last week of Ramadan for three consecutive days one day of which will coincide with the weekend and what should be considered during recording will be explained with examples in advance
A physical activity record form will be filled out to determine the 24-hour physical activity status of the individuals included in the study and daily energy expenditure will be calculated
With the start of the study all participants will be asked about their food consumption records for 3 days 2 days on weekdays 1 day on weekends anthropometric measurements body weight height waist circumference hip circumference and 24-hour physical activity records one week before the start of Ramadan fasting and the last week of Ramadan
One day before and on the last day of Ramadan fasting fecal and blood samples will be collected and total microbiota FABP4 CRP fasting insulin fasting blood glucose HDL-C LDL-C Total-C TG ALT AST and GGT values will be analyzed and recorded
Descriptive statistics will be given as number and percentage for categorical variables and arithmetic mean and standard deviation for continuous variables Chi-square test will be used to compare categorical variables Whether the research data are suitable for normal distribution or not will be tested with Kolmogorov-Smirnov analysis method and when parametric test assumptions are met in the comparison of independent group differences t test and Analysis of Variance will be used in independent groups Mann-Whitney U test and Kruskal Wallis tests will be used when parametric test assumptions are not met In the comparison of dependent groups if parametric assumptions are met t test and repeated measures analysis of variance will be used Wilcoxon test and Friedman test will be used when parametric test assumptions are not met Statistical significance level p005 will be accepted
Intermittent fasting is supported by increasing studies showing that it has a purifying effect on health In particular fasting practices exceeding 16 hours induce autophagy at the cellular level and this continues its effect in all tissue and organ systems after the regeneration movement that starts at the cellular level
One of the important factors affected by intermittent fasting is the changes in microbiota which is closely related to the general health of humans Microbiota is generally defined as the colonization of certain places in our body by various populations of microorganisms and is affected by many factors such as nutrition lifestyle exercise drug use and stress
Fatty acid binding protein 4 FABP4 is an adipocyte member of the fatty acid binding protein FABP family with nine different isoforms It is highly secreted from adipose tissue but is also expressed from monocytes macrophages and dendritic cells This adipocyte which increases in obesity is found at higher levels in women than in men Studies have shown that increased serum FABP4 levels are associated with Type 1 and Type 2 diabetes mellitus DM insulin resistance and cardiovascular diseases
The aim of this thesis study was to investigate the effect of Ramadan fasting on gut microbiota and FABP4
The hypotheses of the study were
1 Gut microbiota composition of adult individuals differs before and after Ramadan fasting
2 Serum FABP4 and other biochemical values of adult individuals differ before and after Ramadan fasting
3 Anthropometric measurements of adults differ before and after Ramadan fasting All individuals who meet the inclusion and exclusion criteria and agree to participate in the study will be read and signed the Informed Voluntary Consent Form and a copy will be delivered to them Questions related to the socio-demographic status and dietary habits of all participants will be asked to them and recorded in the questionnaire form by the researcher using the face-to-face interview technique
Information about the volunteers who participated in the study such as not having any disease DM hypertension dyslipidemia cancer chronic renal failure GI problems etc not taking medication and not having used antibiotics in the last six months were evaluated by the Specialist Dr who took part in the study Dr evaluated the participants through a preliminary interview with the participants Factors such as the fact that the volunteers who participated in the study did not drink alcohol had not applied for any diet program in the last six months whether the participants were doing sahur or not and especially that all participants had the same duration of fasting during the day during Ramadan were evaluated by the researcher through a preliminary interview with the participants All individuals who met the inclusion and exclusion criteria and agreed to participate in the study were read and signed the Informed Voluntary Consent Form and a copy was delivered to them
In order to evaluate the nutritional status of the individuals the participants will be asked to record the amount of food consumption one week before the start of Ramadan fasting and during the last week of Ramadan for three consecutive days one day of which will coincide with the weekend and what should be considered during recording will be explained with examples in advance
A physical activity record form will be filled out to determine the 24-hour physical activity status of the individuals included in the study and daily energy expenditure will be calculated
With the start of the study all participants will be asked about their food consumption records for 3 days 2 days on weekdays 1 day on weekends anthropometric measurements body weight height waist circumference hip circumference and 24-hour physical activity records one week before the start of Ramadan fasting and the last week of Ramadan
One day before and on the last day of Ramadan fasting fecal and blood samples will be collected and total microbiota FABP4 CRP fasting insulin fasting blood glucose HDL-C LDL-C Total-C TG ALT AST and GGT values will be analyzed and recorded
Descriptive statistics will be given as number and percentage for categorical variables and arithmetic mean and standard deviation for continuous variables Chi-square test will be used to compare categorical variables Whether the research data are suitable for normal distribution or not will be tested with Kolmogorov-Smirnov analysis method and when parametric test assumptions are met in the comparison of independent group differences t test and Analysis of Variance will be used in independent groups Mann-Whitney U test and Kruskal Wallis tests will be used when parametric test assumptions are not met In the comparison of dependent groups if parametric assumptions are met t test and repeated measures analysis of variance will be used Wilcoxon test and Friedman test will be used when parametric test assumptions are not met Statistical significance level p005 will be accepted
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None