Ignite Creation Date:
2024-05-06 @ 7:36 PM
Last Modification Date:
2024-10-26 @ 3:10 PM
Study NCT ID:
NCT06066034
Status:
RECRUITING
Last Update Posted:
2023-10-04
First Post:
2023-09-25
Brief Title:
Changes in Serum Follicular Fluid SREBP1c and LRG1 Levels in PCOS Patients and Correlation With Insulin Resistance
Sponsor:
Gui Xiaoting
Organization:
Zunyi Medical College
Study Overview
Official Title:
Changes in Serum Follicular Fluid SREBP1c and LRG1 Levels in PCOS Patients and Correlation With Insulin Resistance
Status:
RECRUITING
Status Verified Date:
2023-09
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Polycystic ovary syndrome PCOS is a common reproductive endocrine and metabolic disease that affects 6-20 of women of childbearing age worldwide Due to changes in modern lifestyles such as low fiber high fat diets sedentary lifestyles smoking and alcohol consumption the risk of developing this disease has increased Its clinical manifestations are heterogeneous with typical clinical manifestations being oligomenorrhea or amenorrhea infertility hirsutism and polycystic ovarian changes under ultrasound Women affected by PCOS face significant reproductive challenges seriously affecting their quality of life and increasing their psychological burdenLRG1 has various regulatory functions including glucose and lipid metabolism IR angiogenesis organ fibrosis and inflammation LRG1 can activate the transcription factor SREBP1 and participate in liver lipid synthesis in obese mice SREBP1c is the active form of SREBP1 Research has shown that SREBP1c can directly or indirectly participate in the development of IR Therefore the investigators speculate that LRG1 may play a certain role in the progression of PCOS through SREBP1cUnderstanding the local changes or metabolic characteristics of follicular fluid in patients with PCOS can help elucidate the pathogenesis of PCOS Therefore in this studythe investigators aim to detect changes in serum and follicular fluid LRG1 SREBP1c and related hormone levels and explore their role in the pathogenesis of PCOS from a systemic and ovarian perspective providing new ideas for the prevention and treatment of PCOS and IR
Detailed Description:
1 The diagnostic criteria for all participants with polycystic ovary syndrome included in the study were based on the 2003 European Society for Human Reproductive and Embryonic Medicine and American Society for Reproductive Medicine ESHREASRM diagnostic criteria in Rotterdam
2 Collect basic data of all participants
3 After informed consent all participants were evaluated for insulin sensitivitythe degree of insulin resistance using the insulin resistance index of the steady-state model All participants were conducted after signing an informed consent form
4 After informed consent all participants were given fasting venous blood samples in the morning for measuring blood sugar blood lipids insulin C-peptide glycosylated hemoglobin HbA1c and a complete set of sex hormones
5 After collecting follicular fluid from all participants centrifuge at 1000xg for 20 minutes filter and precipitate and freeze in a refrigerator at -80 C for the determination of LRG1 SREBP1c and related hormone levels in follicular fluid
6 Use SPSS 220 statistical software for data analysis
2 Collect basic data of all participants
3 After informed consent all participants were evaluated for insulin sensitivitythe degree of insulin resistance using the insulin resistance index of the steady-state model All participants were conducted after signing an informed consent form
4 After informed consent all participants were given fasting venous blood samples in the morning for measuring blood sugar blood lipids insulin C-peptide glycosylated hemoglobin HbA1c and a complete set of sex hormones
5 After collecting follicular fluid from all participants centrifuge at 1000xg for 20 minutes filter and precipitate and freeze in a refrigerator at -80 C for the determination of LRG1 SREBP1c and related hormone levels in follicular fluid
6 Use SPSS 220 statistical software for data analysis
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None