Ignite Creation Date:
2024-05-06 @ 7:30 PM
Last Modification Date:
2024-10-26 @ 3:08 PM
Study NCT ID:
NCT06032117
Status:
RECRUITING
Last Update Posted:
2024-04-23
First Post:
2023-07-28
Brief Title:
Characterizing Protein Biomarkers of Post-infection Irritable Bowel Syndrome
Sponsor:
University of Nevada Reno
Organization:
University of Nevada Reno
Study Overview
Official Title:
Characterizing Protein Biomarkers of Post-infection Irritable Bowel Syndrome
Status:
RECRUITING
Status Verified Date:
2024-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The investigators will characterize and compare protein signatures between groups with and without post-infection irritable bowel syndrome PI-IBS From previous Healthy Nevada Project HNP participants at least 60 patients with PI-IBS and 60 healthy controls will undergo additional proteomics testing age sex and raceethnicity-matched healthy The investigators will use proteomic testing to detect quantify and characterize serum protein biomarkers and protein signatures and compare biomarkers and signatures between the patient groups of interest Serum samples will be analyzed by the Nevada Proteomics Center Samples will first undergo protein digestion then peptides are separated using liquid chromatography LC mass spectral analysis is performed using an Orbitrap Eclipse mass spectrometer Thermo Scientific San Jose CA using data-independent acquisition DIA Library generation and data analysis will be performed using Spectronaut software Biognosys Schlieren Switzerland The Nevada Proteomics Center and Bioinformatics Center will be engaged during the data analyses comparing biomarkers and signatures between the patient groups of interest This research aim has the potential to add to our understanding of the underlying mechanisms of PI-IBS and to create reliable differentiating protein biomarkers to better diagnose PI-IBS
Detailed Description:
The investigators will characterize and compare protein signatures between groups with and without PI-IBS From previous HNP participants at least 60 patients with PI-IBS and 60 healthy controls will be recruited to undergo additional proteomics testing age sex and raceethnicity-matched healthy The investigators will use proteomic testing to detect quantify and characterize serum protein biomarkers and protein signatures and compare biomarkers and signatures between the patient groups of interest Serum samples will be analyzed by the Nevada Proteomics Center Samples will first undergo protein digestion then peptides are separated using liquid chromatography LC mass spectral analysis is performed using an Orbitrap Eclipse mass spectrometer Thermo Scientific San Jose CA using data-independent acquisition DIA Library generation and data analysis will be performed using Spectronaut software Biognosys Schlieren Switzerland The Nevada Proteomics Center and Bioinformatics Center will be engaged during the data analyses comparing biomarkers and signatures between the patient groups of interest This research aim has the potential to add to our understanding of the underlying mechanisms of PI-IBS and to create reliable differentiating protein biomarkers to better diagnose PI-IBS
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None