Ignite Creation Date:
2024-05-06 @ 7:10 PM
Last Modification Date:
2024-10-26 @ 3:01 PM
Study NCT ID:
NCT05916443
Status:
NOT_YET_RECRUITING
Last Update Posted:
2023-07-24
First Post:
2023-05-08
Brief Title:
Dissecting the Biology of Early-onset Colorectal Cancer
Sponsor:
Fondazione Policlinico Universitario Agostino Gemelli IRCCS
Organization:
Fondazione Policlinico Universitario Agostino Gemelli IRCCS
Study Overview
Official Title:
Study of the Biology of Colorectal Cancer in Early-onset Patients
Status:
NOT_YET_RECRUITING
Status Verified Date:
2023-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Contrarily to late-onset LO colorectal cancer CRC early-onset EO CRC incidence is increasingly growing Several factors such as obesity chronic inflammation and intestinal dysbiosis can increase the general risk of CRC However little is known about the biology of EO-CRC To evaluate whether such selective rise in the incidence of EO-CRC patients mirrors a distinct transcriptomic profile the investigators will first dissect EO-CRCs transcriptomic landscape
Then the investigators will investigate the colorectal cancer stem cell CSC compartment by in vitro functional assays and RNA-seq analysis Because our preliminary data indicate an increased aggressiveness of the tumor microenvironment TME in EO-CRCthe investigators propose to investigate the CSC niche and the interaction with the TME to dissect the molecular and cellular pathways occurring in EO-CRC
A cohort of 30 EO-CRC patients 50 years old will be enrolled and fully characterized About 10 EO-CRC-derived CSCs in the form of organoids and spheroids will be generated Since the relevant differences between CR-CSCs isolated from EO-CRC vs LO-CRC patients are still unknown the investigators will gain information about their specific features such as clonogenic activity tumorigenicinvasive capacity and about differences in the mechanisms regulating their cross-talk with TME components
Then the investigators will investigate the colorectal cancer stem cell CSC compartment by in vitro functional assays and RNA-seq analysis Because our preliminary data indicate an increased aggressiveness of the tumor microenvironment TME in EO-CRCthe investigators propose to investigate the CSC niche and the interaction with the TME to dissect the molecular and cellular pathways occurring in EO-CRC
A cohort of 30 EO-CRC patients 50 years old will be enrolled and fully characterized About 10 EO-CRC-derived CSCs in the form of organoids and spheroids will be generated Since the relevant differences between CR-CSCs isolated from EO-CRC vs LO-CRC patients are still unknown the investigators will gain information about their specific features such as clonogenic activity tumorigenicinvasive capacity and about differences in the mechanisms regulating their cross-talk with TME components
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None