Ignite Creation Date:
2024-05-06 @ 6:53 PM
Last Modification Date:
2024-10-26 @ 2:56 PM
Study NCT ID:
NCT05817448
Status:
NOT_YET_RECRUITING
Last Update Posted:
2023-05-24
First Post:
2023-04-05
Brief Title:
Hypoxia-induced Autophagy in the Pathogenesis of MAP
Sponsor:
Assiut University
Organization:
Assiut University
Study Overview
Official Title:
The Possible Role of Hypoxia-induced Autophagy and Matrix Metalloproteinase-9 in the Pathogenesis of Morbidly Adherent Placenta
Status:
NOT_YET_RECRUITING
Status Verified Date:
2023-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
To investigate the role of HIF 1α and LC3B in the pathogenesis of MAP to evaluate the role of MMP-9 in the antenatal prediction of MAP and to compare the expression of HIF1α LC3B and level MMP-9 between patients of placenta previa with MAP and patients with normal placentation
Detailed Description:
Cesarean section CS rate has been rising rapidly in the recent yearsOne of the complications of repeated CS is placenta previa an implantation of placenta on or near internal os
Morbidly adherent placenta MAP known also as placenta accreta spectrum has histopathologic and clinical types The placenta becomes abnormally adherent to the uterine wall fails to separate leading to massive blood loss and possible hysterectomy The risk of MAP in patient with placenta previa increases with repeated CS
Theories for development of MAP include abnormal trophoblastic invasion of myometrium abnormal decidualization neovascularization and reduction of apoptosis of trophobalsts
In early pregnancy the trophoblasts invade spiral arteries and reduce blood flow to the placenta 00leading to hypoxia Hypoxia inducible factor 1 alpha HIF-1α a protein involved in cellular adaptation to hypoxia increases throughout pregnancy and contributes in the invasion of trophoblasts Excessive invasion in MAP might prolong the hypoxia increasing the expression of HIF1α
Autophagy is a process for managing and removing the damaged organelles and cellular proteins in hypoxia It supports the trophoblasts Hypoxia induced autophagy markers such as LC3B might be enhanced by MAP
Matrix metalloproteinase-9 MMP-9 plays an important role in cell invasion and placental implantation Disturbance of MMP-9 might alter the trophobalsts invasion resulting in MAP
The investigators hypothesize that hypoxia autophagy and invasiveness are linked to the pathophysiology of MAP so we will assess the expression of HIF1α LC3B to estimate their role beside MMP-9
Morbidly adherent placenta MAP known also as placenta accreta spectrum has histopathologic and clinical types The placenta becomes abnormally adherent to the uterine wall fails to separate leading to massive blood loss and possible hysterectomy The risk of MAP in patient with placenta previa increases with repeated CS
Theories for development of MAP include abnormal trophoblastic invasion of myometrium abnormal decidualization neovascularization and reduction of apoptosis of trophobalsts
In early pregnancy the trophoblasts invade spiral arteries and reduce blood flow to the placenta 00leading to hypoxia Hypoxia inducible factor 1 alpha HIF-1α a protein involved in cellular adaptation to hypoxia increases throughout pregnancy and contributes in the invasion of trophoblasts Excessive invasion in MAP might prolong the hypoxia increasing the expression of HIF1α
Autophagy is a process for managing and removing the damaged organelles and cellular proteins in hypoxia It supports the trophoblasts Hypoxia induced autophagy markers such as LC3B might be enhanced by MAP
Matrix metalloproteinase-9 MMP-9 plays an important role in cell invasion and placental implantation Disturbance of MMP-9 might alter the trophobalsts invasion resulting in MAP
The investigators hypothesize that hypoxia autophagy and invasiveness are linked to the pathophysiology of MAP so we will assess the expression of HIF1α LC3B to estimate their role beside MMP-9
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None