Ignite Creation Date:
2024-05-06 @ 6:52 PM
Last Modification Date:
2024-10-26 @ 2:56 PM
Study NCT ID:
NCT05814861
Status:
NOT_YET_RECRUITING
Last Update Posted:
2023-04-18
First Post:
2023-03-20
Brief Title:
Isolation and Characterization of Blastocystis Hominis as Apotential Human Pathogen
Sponsor:
Assiut University
Organization:
Assiut University
Study Overview
Official Title:
Isolation and Characterization of Blastocystis Hominis as Apotential Human Pathogen
Status:
NOT_YET_RECRUITING
Status Verified Date:
2023-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Detection of the presence of Blastocystis hominis among outpatients presented with GIT symptoms at Assiut University Hospitals in comparison with those in control group
Assessment of different culture techniques for detection and isolation of Blastocystis hominis
Molecular identification of Blastocystis hominis using polymerase chain reaction PCR
Assessment of different culture techniques for detection and isolation of Blastocystis hominis
Molecular identification of Blastocystis hominis using polymerase chain reaction PCR
Detailed Description:
Blastocystis is a genus of single-celled parasites belonging to a group of organisms that are known as the Stramenopiles Blastocystis consists of several species living in the gastrointestinal tracts of humans and farm animals Many different species can infect humans and would be identified as Blastocystis hominis depending on the sequencing of the gene encoding the 18S rRNA Among the subtypes recognized ST1-8 could colonize humans as well as animals
Infection with B hominis has a worldwide distribution with infection rate reaches up to 60 in developing countries Initially Bhominis was accepted as a harmless commensal in the intestinal tract but recently it was implicated as a pathogen As it able to produce a cysteine protease which breaks up the mucosal IgA antibody Bhominis infection has been associated with several gastrointestinal symptoms Even though most cases are asymptomatic it is also play a significant role in some chronic gastrointestinal illnesses as irritable bowel syndrome IBS
Blastocystis spp are polymorphic parasites with multiple morphological forms including vacuolar multivacuolar granular ameboid and cystic forms At least two out of three infections may be missed during microscopic detection of Bhominis due to their polymorphic nature and the possibility of confusion with yeast Cyclospora spp or fat globules Different studies suggested that it multiply rapidly in culture medium and considered it a sensitive method for diagnosis of Bhominis Recently Molecular diagnosis by polymerase chain reaction PCR is considered the most sensitive method for the detection of Bhominis
Infection with B hominis has a worldwide distribution with infection rate reaches up to 60 in developing countries Initially Bhominis was accepted as a harmless commensal in the intestinal tract but recently it was implicated as a pathogen As it able to produce a cysteine protease which breaks up the mucosal IgA antibody Bhominis infection has been associated with several gastrointestinal symptoms Even though most cases are asymptomatic it is also play a significant role in some chronic gastrointestinal illnesses as irritable bowel syndrome IBS
Blastocystis spp are polymorphic parasites with multiple morphological forms including vacuolar multivacuolar granular ameboid and cystic forms At least two out of three infections may be missed during microscopic detection of Bhominis due to their polymorphic nature and the possibility of confusion with yeast Cyclospora spp or fat globules Different studies suggested that it multiply rapidly in culture medium and considered it a sensitive method for diagnosis of Bhominis Recently Molecular diagnosis by polymerase chain reaction PCR is considered the most sensitive method for the detection of Bhominis
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None