Ignite Creation Date:
2024-05-06 @ 6:50 PM
Last Modification Date:
2024-10-26 @ 2:55 PM
Study NCT ID:
NCT05806372
Status:
NOT_YET_RECRUITING
Last Update Posted:
2023-08-24
First Post:
2023-03-28
Brief Title:
Biomarkers of CVD Dysfunction in Hypertensive Disorders of Pregnancy
Sponsor:
IRCCS Burlo Garofolo
Organization:
IRCCS Burlo Garofolo
Study Overview
Official Title:
Biomarkers of Maternal Cardio-vascular Dysfunction in Pregnancies Complicated by Hypertensive Disorders of Pregnancy
Status:
NOT_YET_RECRUITING
Status Verified Date:
2023-08
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Profound and concomitant cardiovascular hemodynamic changes necessary to support fetoplacental development and its increasing supply demands occur during a physiological pregnancy characterized by an increase in cardiac output heart rate and plasma volume and fall in vascular resistance and blood pressure The result of these changes is a volume overload that will lead to a compensatory transient left ventricular eccentric hypertrophy This together with the pro-inflammatory state typical of pregnancy represents the pregnancy as a stress-test for the maternal cardiovascular system Pregnancies complicated by hypertensive disorders of pregnancy HDP particularly those with early onset andor complicated by intrauterine fetal growth restriction FGR are characterized by a cardiovascular maladaptation Women who experienced HDP in pregnancy especially pre-eclampsia PE more often develop later in life ischemic heart disease hypertension and stroke obesity dyslipidemia and end-stage renal disease
Regardless its clinical impact very little knowledge is available on the mechanisms by which PE could lead to cardiovascular disease CVD and especially to heart failure after pregnancy Preliminary results suggest a cross-talk between pregnancy-induced biomarkers and cardio-vascular system Particularly cultures of neonatal rat cardiomyocytes and fibroblasts were used to investigate the role of the serum of women with HDP in regulating their proliferation 5-ethynyl-2-deoxyuridine EdU was administered to label DNA synthesis in proliferating cells After 3 days of in vitro culture EdU incorporation was analyzed upon immunofluorescence staining using specific antibodies by high content microscopy A possible protective effect exerted by the selected sera against apoptosis was evaluated as well by Caspase activation Moreover the effect of cardiomyocytes and fibroblasts proliferation and apoptosis on maternal hemodynamic parameters was evaluated using median regression models These data show that the serum of women with HDP triggers a net increase in the percentage of proliferating cardiomyocytes compared to controls Moreover there were relationship between cardiomyocytes and fibroblasts proliferation and maternal hemodynamics parameters thus supporting the hypothesis that the serum of women with HDP may contain factors capable of stimulating cardiac cells in response to the cardiovascular stress-test
Regardless its clinical impact very little knowledge is available on the mechanisms by which PE could lead to cardiovascular disease CVD and especially to heart failure after pregnancy Preliminary results suggest a cross-talk between pregnancy-induced biomarkers and cardio-vascular system Particularly cultures of neonatal rat cardiomyocytes and fibroblasts were used to investigate the role of the serum of women with HDP in regulating their proliferation 5-ethynyl-2-deoxyuridine EdU was administered to label DNA synthesis in proliferating cells After 3 days of in vitro culture EdU incorporation was analyzed upon immunofluorescence staining using specific antibodies by high content microscopy A possible protective effect exerted by the selected sera against apoptosis was evaluated as well by Caspase activation Moreover the effect of cardiomyocytes and fibroblasts proliferation and apoptosis on maternal hemodynamic parameters was evaluated using median regression models These data show that the serum of women with HDP triggers a net increase in the percentage of proliferating cardiomyocytes compared to controls Moreover there were relationship between cardiomyocytes and fibroblasts proliferation and maternal hemodynamics parameters thus supporting the hypothesis that the serum of women with HDP may contain factors capable of stimulating cardiac cells in response to the cardiovascular stress-test
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None