Ignite Creation Date:
2024-05-06 @ 6:50 PM
Last Modification Date:
2024-10-26 @ 2:55 PM
Study NCT ID:
NCT05801185
Status:
ENROLLING_BY_INVITATION
Last Update Posted:
2024-06-21
First Post:
2023-03-13
Brief Title:
Single Nuclei Sequencing in Human Skeletal Muscle After Unloading
Sponsor:
Karolinska Institutet
Organization:
Karolinska Institutet
Study Overview
Official Title:
Single Nuclei Sequencing in Human Skeletal Muscle After Unloading
Status:
ENROLLING_BY_INVITATION
Status Verified Date:
2024-06
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
ULLS23
Brief Summary:
Injuries often require people to undergo some degree of limb immobilization This comes at a high cost in terms of muscle mass and function losses At the molecular level it is completely unknown how the different cells in the muscle respond to this complete lack of mechanical stimuli
The investigators will explore cell type specific changes in skeletal muscle after a short period of unloading and specifically examine the behavior of myogenic stem cells with particular attention to potential unloading-induced maturation into fast- or slow-twitch muscle fibers
Ten healthy young participants will perform a 5-day Unilateral Lower Limb Suspension ULLS intervention where one leg is continuously subjected to unloading ie lack of mechanical stimuli Before and after the ULLS period participants will perform muscle function testing and MRI scans to assess muscle size in the lower limbs Immediately after the ULLS two muscle biopsies will be obtained one from each leg The tissue will be employed for single-nuclei RNA sequencing analysis and immunohistochemistry experiments
This project will be the first to analyze cell type specific changes induced by complete short-term lack of mechanical stimuli in skeletal muscle This will add a new dimension to the understanding of the processes directing this type of muscle atrophy ie muscle loss in otherwise healthy individuals and thus help to develop effective countermeasures to offset muscle changes
The investigators will explore cell type specific changes in skeletal muscle after a short period of unloading and specifically examine the behavior of myogenic stem cells with particular attention to potential unloading-induced maturation into fast- or slow-twitch muscle fibers
Ten healthy young participants will perform a 5-day Unilateral Lower Limb Suspension ULLS intervention where one leg is continuously subjected to unloading ie lack of mechanical stimuli Before and after the ULLS period participants will perform muscle function testing and MRI scans to assess muscle size in the lower limbs Immediately after the ULLS two muscle biopsies will be obtained one from each leg The tissue will be employed for single-nuclei RNA sequencing analysis and immunohistochemistry experiments
This project will be the first to analyze cell type specific changes induced by complete short-term lack of mechanical stimuli in skeletal muscle This will add a new dimension to the understanding of the processes directing this type of muscle atrophy ie muscle loss in otherwise healthy individuals and thus help to develop effective countermeasures to offset muscle changes
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None