Ignite Creation Date:
2024-05-06 @ 6:24 PM
Last Modification Date:
2024-10-26 @ 2:47 PM
Study NCT ID:
NCT05658445
Status:
RECRUITING
Last Update Posted:
2022-12-29
First Post:
2022-12-12
Brief Title:
Potential Role of microRNA 410 and BIRC7 Pathways in Recurrent Spontaneous Miscarriage
Sponsor:
Nesma Gamal
Organization:
Assiut University
Study Overview
Official Title:
Potential Role of microRNA 410 and BIRC7 Pathways in Recurrent Spontaneous Miscarriage
Status:
RECRUITING
Status Verified Date:
2023-12
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
microRNA410
Brief Summary:
The definition of recurrent spontaneous abortion RSA has changed over the years and most societies now advocate defining RSA as two or three consecutive or discontinuous miscarriages with the same sexual partner before 24 weeks gestation
In recent years the incidence of this disease has been on the rise occurring in about 1- 5 of pregnancy in women at childbearing age and the success rate of second pregnancy in RSA females has been significantly reduced The etiology of RSA is extremely complex including anatomical factors genetic factors endocrine factors infectious and immune factors and pre-thrombosis etiology
However the cause of the disease is unclear in half of patients and known as unexplained recurrent spontaneous abortion URSA
In recent years the incidence of this disease has been on the rise occurring in about 1- 5 of pregnancy in women at childbearing age and the success rate of second pregnancy in RSA females has been significantly reduced The etiology of RSA is extremely complex including anatomical factors genetic factors endocrine factors infectious and immune factors and pre-thrombosis etiology
However the cause of the disease is unclear in half of patients and known as unexplained recurrent spontaneous abortion URSA
Detailed Description:
MicroRNA miRNA is a kind of non-coding single-stranded RNA molecule encoded by endogenous genes with a length of about 22 nucleotides MiRNA negatively regulates gene expression mainly by binding to the 3 untranslated region 3 UTR of target mRNA to degrade the target mRNA or inhibit its translation In recent years there have been numerous reports about miRNAs involvement in the pathogenesis of RSA and many of them have focused on how miRNA regulates trophoblast function
Additionally microRNA miR-410-5p has been discovered to serve as oncogenes and tumor suppressors altering cell functions in numerous ways including proliferation apoptosis biochemistry metabolism and inflammatory responses in different human malignancies such as liver cancer pancreatic cancer colon cancer and non-small cell lung cancer Recent studies showed that miR-410-5p may be involved in the non-cancerous disorder processes such as RSA Overexpression of miR-410-5p in trophoblast cells inhibited the polarization of M2 macrophages while knockdown of miR-410-5p was beneficial to recruitment of trophoblast cell and promoted the polarization of M2 macrophages Furthermore MicroRNA miR-410-5p was discovered to bind with 3-UTR of ITGA6
The adhesion molecule integrin alpha-6 ITGA6 CD49f a member of the integrin family is overexpressed in many cancers and enhances cell movement and signal output
Down-regulation of ITGA6 changed the biological function of trophoblast cells inhibited cell proliferation invasion and migration and induced apoptosis ITGA6 may affect the biological functions of trophoblast cells by regulating PI3KAKT and MAPK signaling pathways MAPK signaling promote apoptosis through inducing releasing of cytochrome c which in turn induces further caspase activation caspase-9 and the effector caspases-3 -6 and -7
Baculoviral IAP Repeat Containing 7BIRC7 encodes the protein Livin which is a member of the inhibitor of apoptosis protein IAP family Livin consists of a single baculoviral IAP repeat domain BIR and a RING domian at the C-terminus The protein inhibits apoptosis by inhibiting proteolytic activation of capsases
Currently the interaction of BIRC7 and NK cells in endometriumdecidual remains unknown BIRC7 in decidua cell may possess the ability to prevent NK cells from killing embryonic and extraembryonic cells decreased level of BIRC7 and increased number of NK cells in the RM group attribute to the occurrence of miscarriage
Additionally microRNA miR-410-5p has been discovered to serve as oncogenes and tumor suppressors altering cell functions in numerous ways including proliferation apoptosis biochemistry metabolism and inflammatory responses in different human malignancies such as liver cancer pancreatic cancer colon cancer and non-small cell lung cancer Recent studies showed that miR-410-5p may be involved in the non-cancerous disorder processes such as RSA Overexpression of miR-410-5p in trophoblast cells inhibited the polarization of M2 macrophages while knockdown of miR-410-5p was beneficial to recruitment of trophoblast cell and promoted the polarization of M2 macrophages Furthermore MicroRNA miR-410-5p was discovered to bind with 3-UTR of ITGA6
The adhesion molecule integrin alpha-6 ITGA6 CD49f a member of the integrin family is overexpressed in many cancers and enhances cell movement and signal output
Down-regulation of ITGA6 changed the biological function of trophoblast cells inhibited cell proliferation invasion and migration and induced apoptosis ITGA6 may affect the biological functions of trophoblast cells by regulating PI3KAKT and MAPK signaling pathways MAPK signaling promote apoptosis through inducing releasing of cytochrome c which in turn induces further caspase activation caspase-9 and the effector caspases-3 -6 and -7
Baculoviral IAP Repeat Containing 7BIRC7 encodes the protein Livin which is a member of the inhibitor of apoptosis protein IAP family Livin consists of a single baculoviral IAP repeat domain BIR and a RING domian at the C-terminus The protein inhibits apoptosis by inhibiting proteolytic activation of capsases
Currently the interaction of BIRC7 and NK cells in endometriumdecidual remains unknown BIRC7 in decidua cell may possess the ability to prevent NK cells from killing embryonic and extraembryonic cells decreased level of BIRC7 and increased number of NK cells in the RM group attribute to the occurrence of miscarriage
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None