Ignite Creation Date:
2024-05-06 @ 6:06 PM
Last Modification Date:
2024-10-26 @ 2:41 PM
Study NCT ID:
NCT05547685
Status:
UNKNOWN
Last Update Posted:
2022-09-21
First Post:
2020-08-30
Brief Title:
Methylene Blue Intra-vital Nerve Staining
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
Harmonic Generation Microscopy Observation of Intradermal Injection Nerve Fibers by Methylene Blue Intra-vital Staining
Status:
UNKNOWN
Status Verified Date:
2022-01
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
In previous studies we know methylene blue MB stains nerves and oxidized MB has near-infrared NIR fluorescence for fluorescent observation In this trial we using MB on human skin and combining MB and third-harmonic generation imaging THG to label cutaneous nerve fibers
Detailed Description:
Intravital images were obtained before injection and then taken at 05 1 15 2 25 and 3 h after intradermal 003 03 mgmL 1500μM MB 10mgmL Astar injection using a 05-mL 29-gauge insulin syringe needle Becton Dickinson and Company USA The participant lay down and the skin was imaged 10 cm above the ankle in the lower leg and above the wrist in the middle forearm three sites in each limb were randomly selected for each time point SHG or THG captured MB signals with two-photon excitation microscope at the Molecular Biomedical Imaging Research Center of National Taiwan University
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None