Ignite Creation Date:
2024-05-05 @ 6:35 PM
Last Modification Date:
2024-10-26 @ 9:34 AM
Study NCT ID:
NCT00501774
Status:
COMPLETED
Last Update Posted:
2007-07-16
First Post:
2007-07-13
Brief Title:
A Search for Helicobacter Pylori in Localized Vulvodynia
Sponsor:
Western Galilee Hospital-Nahariya
Organization:
Western Galilee Hospital-Nahariya
Study Overview
Official Title:
A Laboratory and Clinical Search for the Presence of H Pylori and Treatment Against it in Localized Vulvodynia Vestibulitis Vestibulodynia
Status:
COMPLETED
Status Verified Date:
2007-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Hypothesis to examine a possible association between localized vulvodynia and H pylori infection
Detailed Description:
Subsequent sections from the paraffin blocks were prepared and stained by modified Giemsa Immunostaining for H pylori was done as described In short tissue sections were deparaffinized in xylene rehydrated through decreasing concentrations of alcohol ending in phosphate-buffered saline PBS and subjected to pretreatment with Proteinase K 8 minutes The sections were quenched with 3 hydrogen peroxidase incubated with protein block for 15 minutes at room temperature and washed in PBS Tissues then were incubated with polyclonal rabbit anti-H pylori antibody dilution 110 clone ch-20 429 DAKO Carpinteria CA Finally sections were washed in 005 polysorbate 20 in PBS pH 74 and the bound antibody was detected using streptavidin and biotinylated secondary antibody with diaminobenzidine as the chromogen Sections were counterstained with hematoxylin dehydrated and mounted Negative controls were sections treated as above but instead of incubation with the primary antibody they were incubated with 1 bovine serum albumin in PBS
Vulvar biopsies of seven other women without localized vulvodynia served as healthy controls
The positive and negative control gastric tissues for the immunohistochemical stain of the H pylori microorganisms were obtained from the archives of the Department of Pathology
Vulvar biopsies of seven other women without localized vulvodynia served as healthy controls
The positive and negative control gastric tissues for the immunohistochemical stain of the H pylori microorganisms were obtained from the archives of the Department of Pathology
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None