Ignite Creation Date:
2024-05-06 @ 5:33 PM
Last Modification Date:
2024-10-26 @ 2:31 PM
Study NCT ID:
NCT05346978
Status:
COMPLETED
Last Update Posted:
2022-04-26
First Post:
2022-04-05
Brief Title:
Nasturtium Tropaeolum Majus L Intake and Biochemical Parameters in Pre-diabetic Subjects in Bogota Colombia
Sponsor:
Javeriana University
Organization:
Javeriana University
Study Overview
Official Title:
Effect of Consumption of a Lyophilized Nasturtium Tropaeolum Majus L on the Insulin Response Lipid Profile Antioxidant Capacity and Gene Expression in Pre-diabetic Subjects Bogotá-Colombia
Status:
COMPLETED
Status Verified Date:
2022-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Brassicaceae plant family have a high content of bioactive compounds such as eg glucosinolates GSLs and isothiocyanates ICTs associated recently with diabetes prevention This research proposal has the intention of evaluating if the ingestion of freeze-dried nasturtium has a positive effect on the insulin response lipid profile oxidative stress biomarkers and gene expression of RESISTIN GLUT 4 acetyl-CoA carboxylase-a ACC fatty acid synthase FASN NRF-2 NQO1 SFRXN1 glutathione peroxidase 2 GPx-2 FOXO1 FOXO3 and FOXO6 in subjects with glucose intolerance
Detailed Description:
Epidemiological and animal studies have shown that consumption of fruits and vegetables decreases the risk of chronic diseases Especially in T2D the primary prevention is considered as a public health priority Some studies show an inverse association between vegetables intake and T2D prevention although the underlying mechanisms and the effect of individual antioxidant compounds are still unclear
Brassicaceae plant family eg broccoli cabbage brussels sprouts watercress Nasturtium garden cress kale cauliflower have a high content of bioactive compounds such as eg glucosinolates GSLs isothiocyanates ICTs and polyphenols associated recently with diabetes prevention 18 Human studies in diabetic patients have shown that the consumption of broccoli sprouts powder BSP containing high concentration of the GLS sulphoraphane for 4 weeks decreases serum insulin concentration homoeostasis model assessment of insulin resistance HOMA-IR LDL inflammation and antioxidant markers Besides sulphoraphane other GLSs have showed a protective effect in diabetes Recently mice fed with very high fat diet VHFD supplemented with 5 of moringa concentrate rich in benzyl isothiocyanate showed that moringa isothiocyanates MICs improved glucose tolerance and insulin signaling reduced plasma leptin resistin cholesterol IL-1β TNFα and lower hepatic glucose-6-phosphatase G6Pase gene expression This evidence suggest that MICs could play a role in T2D prevention probably by inhibiting rate-limiting steps in liver gluconeogenesis resulting in direct or indirect increase in insulin signaling and sensitivity
Recently in vitro studies in human and osteosarcoma hepatoma cells HepG2 cells stimulated with benzyl isothiocyanate BITC from Tropaeolum majus show that this compound is able of 1 regulate the insulin signaling pathway 2 down-regulate the gene and protein expression of gluconeogenic enzymes G6Pase and phosphoenol piruvatokinase PEPCK and 3 up-regulate the gene expression of antioxidant and phase II detoxification genes manganese superoxide dismutase MnSOD nuclear factor erythroid derived-like2 NRF2 NADPH dehydrogenase quinone 1 NQO1 and sulfiredoxin-1 SFRXN1 21 According with this evidence BITC could mimics the fasting state in which insulin stimuli is absent and transcription factors remain in the nucleus modulating gene expression of their target genes with the advantage of down-regulating gluconeogenesis instead of increase it suggesting that BITC could have a therapeutic role in the prevention or treatment of T2D
Despite of strong evidence of 1 chronic inflammation as an underlying cause of T2D and 2 high levels of oxidative stress under T2D conditions the use of ITC-rich foods as therapeutics in T2D remains virtually unknown For that reason this research proposal has the intention of evaluating if the ingestion of freeze-dried nasturtium has a positive effect on the insulin response lipid profile oxidative stress biomarkers and gene expression of RESISTIN GLUT 4 acetyl-CoA carboxylase-a ACC fatty acid synthase FASN NRF-2 NQO1 SFRXN1 glutathione peroxidase 2 GPx-2 FOXO1 FOXO3 and FOXO6 in subjects with glucose intolerance
Brassicaceae plant family eg broccoli cabbage brussels sprouts watercress Nasturtium garden cress kale cauliflower have a high content of bioactive compounds such as eg glucosinolates GSLs isothiocyanates ICTs and polyphenols associated recently with diabetes prevention 18 Human studies in diabetic patients have shown that the consumption of broccoli sprouts powder BSP containing high concentration of the GLS sulphoraphane for 4 weeks decreases serum insulin concentration homoeostasis model assessment of insulin resistance HOMA-IR LDL inflammation and antioxidant markers Besides sulphoraphane other GLSs have showed a protective effect in diabetes Recently mice fed with very high fat diet VHFD supplemented with 5 of moringa concentrate rich in benzyl isothiocyanate showed that moringa isothiocyanates MICs improved glucose tolerance and insulin signaling reduced plasma leptin resistin cholesterol IL-1β TNFα and lower hepatic glucose-6-phosphatase G6Pase gene expression This evidence suggest that MICs could play a role in T2D prevention probably by inhibiting rate-limiting steps in liver gluconeogenesis resulting in direct or indirect increase in insulin signaling and sensitivity
Recently in vitro studies in human and osteosarcoma hepatoma cells HepG2 cells stimulated with benzyl isothiocyanate BITC from Tropaeolum majus show that this compound is able of 1 regulate the insulin signaling pathway 2 down-regulate the gene and protein expression of gluconeogenic enzymes G6Pase and phosphoenol piruvatokinase PEPCK and 3 up-regulate the gene expression of antioxidant and phase II detoxification genes manganese superoxide dismutase MnSOD nuclear factor erythroid derived-like2 NRF2 NADPH dehydrogenase quinone 1 NQO1 and sulfiredoxin-1 SFRXN1 21 According with this evidence BITC could mimics the fasting state in which insulin stimuli is absent and transcription factors remain in the nucleus modulating gene expression of their target genes with the advantage of down-regulating gluconeogenesis instead of increase it suggesting that BITC could have a therapeutic role in the prevention or treatment of T2D
Despite of strong evidence of 1 chronic inflammation as an underlying cause of T2D and 2 high levels of oxidative stress under T2D conditions the use of ITC-rich foods as therapeutics in T2D remains virtually unknown For that reason this research proposal has the intention of evaluating if the ingestion of freeze-dried nasturtium has a positive effect on the insulin response lipid profile oxidative stress biomarkers and gene expression of RESISTIN GLUT 4 acetyl-CoA carboxylase-a ACC fatty acid synthase FASN NRF-2 NQO1 SFRXN1 glutathione peroxidase 2 GPx-2 FOXO1 FOXO3 and FOXO6 in subjects with glucose intolerance
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None