Ignite Creation Date:
2024-05-06 @ 5:18 PM
Last Modification Date:
2024-10-26 @ 2:26 PM
Study NCT ID:
NCT05269901
Status:
COMPLETED
Last Update Posted:
2022-04-15
First Post:
2022-02-23
Brief Title:
Association Between Ferroptosis and Epilepsy
Sponsor:
Affiliated Hospital of Jiangnan University
Organization:
Affiliated Hospital of Jiangnan University
Study Overview
Official Title:
Association Between GPX4 Dependent Ferroptosis Pathway and Epilepsy in School-aged Children
Status:
COMPLETED
Status Verified Date:
2022-02
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Epilepsy is one of the most common neurologic disorders seen in children often characterized by recurring seizures Nearly 105 million children worldwide are estimated to have active epilepsy Children with epilepsy are more likely to have developmental health and developmental comorbidities such as depression anxiety attention deficit hyperactivity disorder learning disabilities and developmental delay compared to children without epilepsy Status epilepticus SE is the most common life-threatening emergency neurological emergency in children and leads to hippocampal neuronal cell death The animal model proved SE-induced neuronal cell death in hippocampal CA1 and CA3 regions Classical drugs like carbamazepine or phenytoin often cause behavioral problems and side effects such as unsteady gait depression and irritability In addition classical medicine did not protect cognitive function and preferred to drive drug-resistant Therefore it is necessary to develop a novel therapy to treat epilepsy Ferroptosis is a new type of cell death usually accompanied by a large amount of iron accumulation and lipid peroxidation It is widely accepted that glutamate-mediated neuronal hyperexcitation plays a causative role in eliciting seizures and cystineglutamate antiporter inhibition induces ferroptosis Hence investigators hypothesize GPX4 dependent ferroptosis pathway may play a key role in eliciting seizures
Detailed Description:
To investigate the possible association between GPX4 dependent ferroptosis pathway and epilepsy Investigators first obtained gene expression of GSE25453 from GEO httpswwwncbinlmnihgovgeo which contained ten medial temporal lobe epilepsy and ten adjacent non-seizure region tissues Then investigators further the possible association between GPX4 dependent ferroptosis pathway and epilepsy in school-aged children Investigators obtained peripheral blood from 20 newly diagnosed untreated school-aged children 6 -12 years and 20 age-matched healthy controls Three glutathione peroxidase 4 GPX4dependent ferroptosis pathway biomarkers were investigated Solute Carrier Family 7 Member 11SLC7A11 GPX4 tumor protein 53 P53 Western blot and Rt-qPCR were used to investigate the possible changes in these three biomarkers
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None