Ignite Creation Date:
2024-05-05 @ 5:33 PM
Last Modification Date:
2024-10-26 @ 9:33 AM
Study NCT ID:
NCT00476658
Status:
COMPLETED
Last Update Posted:
2011-12-07
First Post:
2007-05-21
Brief Title:
Macrophage Infiltration in Human Adipose Tissue
Sponsor:
Assistance Publique - Hôpitaux de Paris
Organization:
Assistance Publique - Hôpitaux de Paris
Study Overview
Official Title:
Characterisation of Macrophage Infiltration in Obesity and Its Complications
Status:
COMPLETED
Status Verified Date:
2007-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Quantification of macrophage infiltration and characterization of macrophage phenotype in adipose tissue of 60 obese subjects and 20 non obese subjects
Detailed Description:
Obesity results from a disequilibrium in energy balance and an inability to adapt to lifestyles which encourage increased energy intake and sedentariness These last years numerous studies contributed to show that obesity is the pathology of an organ the white adipose tissue AT characterized by a low grade inflammation when it is enlarged The fact that adipocytes secrete a number of inflammatory factors or adipokines has forced several groups to reassess the involvement of the AT in wide range of physiological and pathophysiological processes Adipose tissue probably contributes to the links between obesity inflammation and insulin-resistance Our previous results based on transcriptomics studies showed that genes encoding inflammatory molecules are mobilized in adipose tissue of obese subjects and that caloric restriction improves greatly inflammatory profile Our preliminary studies on the morphology of adipose tissue lead us to observe a major infiltration of macrophages in morbidly obese subjects This project is based on a clinical protocol performed in massively obese subjects BMI40 kgm² where drastic weight loss is achieved by gastric bypass This project has several objectives It will allow us to characterize the infiltration of macrophages in the subcutaneous adipose tissue of 60 obese subjects compared with 20 normal-weight subjects to study the evolution during surgically-induced weight loss and to compare the infiltration observed in adipose tissue to that of other tissues visceral adipose tissue muscle liver bowel and stomach This study will be not only quantitative number of macrophages but also qualitative by characterizing the phenotype of macrophages with transcriptomics and immunohistochemical approaches We will analyze the relationships between morphological characteristics of adipose tissue and clinical and biochemical parameters related to insulin sensitivity More generally this project might lead us to go thoroughly into the knowledge of the link between obesity and associated comorbidities particularly metabolic complications and to consider novels therapeutic strategies
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None