Ignite Creation Date:
2024-05-06 @ 5:08 PM
Last Modification Date:
2024-10-26 @ 2:22 PM
Study NCT ID:
NCT05202548
Status:
COMPLETED
Last Update Posted:
2022-01-21
First Post:
2022-01-08
Brief Title:
Immunology Dysregulation in Lymphadenitis Tuberculosis An Observational Study Using Patient Block Paraffins 2019 Until 2021
Sponsor:
Universitas Katolik Widya Mandala Surabaya
Organization:
Universitas Katolik Widya Mandala Surabaya
Study Overview
Official Title:
Immunology Dysregulation in Lymphadenitis Tuberculosis An Observational Study Using Patient Block Paraffins 2019 Until 2021
Status:
COMPLETED
Status Verified Date:
2022-01
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Mycobacterium tuberculosis M tuberculosis infection is still a problem that cannot be overcome in Indonesia In 2018 the number of tuberculosis TB sufferers in Indonesia reached more than 850100000 population an increase around 20 from the previous years incidence One of the highest number of cases is in East Java Province which is 38 of the total number of new TB cases in Indonesia
Extrapulmonary tuberculosis EPTB according to WHO classification criteria is an M tuberculosis infection that occurs in tissues and organs outside the lung parenchyma The incidence rate in Indonesia reaches 1-5 of the incidence of TB thus EPTB may often be forgotten However the diagnosis therapy and monitoring post treatment in EPTB remains difficult to do
The focus of this research is tuberculous lymphadenitis TB due to 50 of EPTB cases in Indonesia was lymphadenitis TB The risk factors for EPTB are immunocompromised conditions such as HIV infection or comorbid conditions such as chronic kidney disease CKD and diabetes mellitus but the mechanism of EPTB homing is still unclear The mechanism of EPTB homing especially TB lymphadenitis really needs to be known for the development of diagnostics and therapy as well as preventing the occurrence of TB lymphadenitis
The importance of this research is to obtain compounds from the human immune response that can be developed as diagnostic markers and therapeutic targets for tuberculosis infection especially TB lymphadenitis Activated macrophages containing M tuberculosis are carried by lymph flow to lymph nodes where there is deposition of antigen-antibody complexes which then activate CC Chemokine Receptor-2 CCR2 on lymphocytes which are the primary receptors for Chemokine CC motif ligands CCL-8 and CCL5 proteins expressed on macrophages containing M tuberculosis Activation of CCR2 increases the production of IL-1010 IL-10 has been responsible for decreasing the secretion of TNF- IFN-γ and IL-1β 11 IFN-γ affects the process of M tuberculosis elimination and the success of TB therapy so that IL-10 is responsible for the failure of macrophages to eliminate M tuberculosis IL-10 also binds to Signal Transducer and Activator of Transcription 3 STAT3 and STAT3 increases the release of Suppressor of Cytokine Signaling 3 SOCS3 SOCS3 interferes with IFN-γ signaling for CCR2 recognition of M tuberculosis-containing macrophages On the other hand the mechanism of T lymphocytes and macrophages that activate pro-inflammatory mediators TNF- IFN-γ and IL-1β and the association of IL-10 activation on STAT3 SOCS3 and CCR2 expression in the incidence of EPTB especially TB lymphadenitis without TB infection remains unknown
Extrapulmonary tuberculosis EPTB according to WHO classification criteria is an M tuberculosis infection that occurs in tissues and organs outside the lung parenchyma The incidence rate in Indonesia reaches 1-5 of the incidence of TB thus EPTB may often be forgotten However the diagnosis therapy and monitoring post treatment in EPTB remains difficult to do
The focus of this research is tuberculous lymphadenitis TB due to 50 of EPTB cases in Indonesia was lymphadenitis TB The risk factors for EPTB are immunocompromised conditions such as HIV infection or comorbid conditions such as chronic kidney disease CKD and diabetes mellitus but the mechanism of EPTB homing is still unclear The mechanism of EPTB homing especially TB lymphadenitis really needs to be known for the development of diagnostics and therapy as well as preventing the occurrence of TB lymphadenitis
The importance of this research is to obtain compounds from the human immune response that can be developed as diagnostic markers and therapeutic targets for tuberculosis infection especially TB lymphadenitis Activated macrophages containing M tuberculosis are carried by lymph flow to lymph nodes where there is deposition of antigen-antibody complexes which then activate CC Chemokine Receptor-2 CCR2 on lymphocytes which are the primary receptors for Chemokine CC motif ligands CCL-8 and CCL5 proteins expressed on macrophages containing M tuberculosis Activation of CCR2 increases the production of IL-1010 IL-10 has been responsible for decreasing the secretion of TNF- IFN-γ and IL-1β 11 IFN-γ affects the process of M tuberculosis elimination and the success of TB therapy so that IL-10 is responsible for the failure of macrophages to eliminate M tuberculosis IL-10 also binds to Signal Transducer and Activator of Transcription 3 STAT3 and STAT3 increases the release of Suppressor of Cytokine Signaling 3 SOCS3 SOCS3 interferes with IFN-γ signaling for CCR2 recognition of M tuberculosis-containing macrophages On the other hand the mechanism of T lymphocytes and macrophages that activate pro-inflammatory mediators TNF- IFN-γ and IL-1β and the association of IL-10 activation on STAT3 SOCS3 and CCR2 expression in the incidence of EPTB especially TB lymphadenitis without TB infection remains unknown
Detailed Description:
Mycobacterium tuberculosis M tuberculosis infection is still a problem that cannot be overcome in Indonesia In 2018 the number of tuberculosis TB sufferers in Indonesia reached more than 850100000 population an increase around 20 from the previous years incidence One of the highest number of cases is in East Java Province which is 38 of the total number of new TB cases in Indonesia
Extrapulmonary tuberculosis EPTB according to WHO classification criteria is an M tuberculosis infection that occurs in tissues and organs outside the lung parenchyma The incidence rate in Indonesia reaches 1-5 of the incidence of TB thus EPTB may often be forgotten However the diagnosis therapy and monitoring post treatment in EPTB remains difficult to do
The focus of this research is tuberculous lymphadenitis TB due to 50 of EPTB cases in Indonesia was lymphadenitis TB The risk factors for EPTB are immunocompromised conditions such as HIV infection or comorbid conditions such as chronic kidney disease CKD and diabetes mellitus but the mechanism of EPTB homing is still unclear The mechanism of EPTB homing especially TB lymphadenitis really needs to be known for the development of diagnostics and therapy as well as preventing the occurrence of TB lymphadenitis
The importance of this research is to obtain compounds from the human immune response that can be developed as diagnostic markers and therapeutic targets for tuberculosis infection especially TB lymphadenitis Activated macrophages containing M tuberculosis are carried by lymph flow to lymph nodes where there is deposition of antigen-antibody complexes which then activate CC Chemokine Receptor-2 CCR2 on lymphocytes which are the primary receptors for Chemokine CC motif ligands CCL-8 and CCL5 proteins expressed on macrophages containing M tuberculosis Activation of CCR2 increases the production of IL-1010 IL-10 has been responsible for decreasing the secretion of TNF- IFN-γ and IL-1β 11 IFN-γ affects the process of M tuberculosis elimination and the success of TB therapy so that IL-10 is responsible for the failure of macrophages to eliminate M tuberculosis IL-10 also binds to Signal Transducer and Activator of Transcription 3 STAT3 and STAT3 increases the release of Suppressor of Cytokine Signaling 3 SOCS3 SOCS3 interferes with IFN-γ signaling for CCR2 recognition of M tuberculosis-containing macrophages On the other hand the mechanism of T lymphocytes and macrophages that activate pro-inflammatory mediators TNF- IFN-γ and IL-1β and the association of IL-10 activation on STAT3 SOCS3 and CCR2 expression in the incidence of EPTB especially TB lymphadenitis without TB infection remains unknown
In this study Lymphadenitis TB paraffin blocks from patients were collected and screened for immunological responses that TB patients have potential as targets for diagnosis and therapy The paraffin block samples came from the Anatomical Pathology Laboratory of St Catholic Hospital Vincentius A Paulo RKZ Surabaya Existing samples were taken and performed immuno-histochemical IHC to define theimmune dysregulation in TB lymphadenitis
Extrapulmonary tuberculosis EPTB according to WHO classification criteria is an M tuberculosis infection that occurs in tissues and organs outside the lung parenchyma The incidence rate in Indonesia reaches 1-5 of the incidence of TB thus EPTB may often be forgotten However the diagnosis therapy and monitoring post treatment in EPTB remains difficult to do
The focus of this research is tuberculous lymphadenitis TB due to 50 of EPTB cases in Indonesia was lymphadenitis TB The risk factors for EPTB are immunocompromised conditions such as HIV infection or comorbid conditions such as chronic kidney disease CKD and diabetes mellitus but the mechanism of EPTB homing is still unclear The mechanism of EPTB homing especially TB lymphadenitis really needs to be known for the development of diagnostics and therapy as well as preventing the occurrence of TB lymphadenitis
The importance of this research is to obtain compounds from the human immune response that can be developed as diagnostic markers and therapeutic targets for tuberculosis infection especially TB lymphadenitis Activated macrophages containing M tuberculosis are carried by lymph flow to lymph nodes where there is deposition of antigen-antibody complexes which then activate CC Chemokine Receptor-2 CCR2 on lymphocytes which are the primary receptors for Chemokine CC motif ligands CCL-8 and CCL5 proteins expressed on macrophages containing M tuberculosis Activation of CCR2 increases the production of IL-1010 IL-10 has been responsible for decreasing the secretion of TNF- IFN-γ and IL-1β 11 IFN-γ affects the process of M tuberculosis elimination and the success of TB therapy so that IL-10 is responsible for the failure of macrophages to eliminate M tuberculosis IL-10 also binds to Signal Transducer and Activator of Transcription 3 STAT3 and STAT3 increases the release of Suppressor of Cytokine Signaling 3 SOCS3 SOCS3 interferes with IFN-γ signaling for CCR2 recognition of M tuberculosis-containing macrophages On the other hand the mechanism of T lymphocytes and macrophages that activate pro-inflammatory mediators TNF- IFN-γ and IL-1β and the association of IL-10 activation on STAT3 SOCS3 and CCR2 expression in the incidence of EPTB especially TB lymphadenitis without TB infection remains unknown
In this study Lymphadenitis TB paraffin blocks from patients were collected and screened for immunological responses that TB patients have potential as targets for diagnosis and therapy The paraffin block samples came from the Anatomical Pathology Laboratory of St Catholic Hospital Vincentius A Paulo RKZ Surabaya Existing samples were taken and performed immuno-histochemical IHC to define theimmune dysregulation in TB lymphadenitis
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None