Ignite Creation Date:
2024-05-06 @ 4:09 PM
Last Modification Date:
2024-10-26 @ 2:04 PM
Study NCT ID:
NCT04888260
Status:
COMPLETED
Last Update Posted:
2021-05-17
First Post:
2021-05-07
Brief Title:
Plasma Mannose Associated Parameter Levels in Nondiabetic Coronary Artery Disease
Sponsor:
Kafkas University
Organization:
Kafkas University
Study Overview
Official Title:
Associations Between Serum Lipids and Mannose Levels in Coronary Artery Disease Among Nondiabetic Patients
Status:
COMPLETED
Status Verified Date:
2021-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Aims Nondiabetic patients have been studied to determine whether modest elevations in plasma mannose levels may be associated with a greater incidence of coronary artery disease CAD
Methods The plasma mannose lipids triglyceride LDL HDL LDL VLDL and LDH levels were successfully will be evaluated with respect to subsequent coronary artery disease using records 120 nondiabetic patients and 120 healthy volunteers CAD was identified from myocardial infarction and new diagnoses of angina
The associations between mannose levels and serum lipid parameters will be investigated
Methods The plasma mannose lipids triglyceride LDL HDL LDL VLDL and LDH levels were successfully will be evaluated with respect to subsequent coronary artery disease using records 120 nondiabetic patients and 120 healthy volunteers CAD was identified from myocardial infarction and new diagnoses of angina
The associations between mannose levels and serum lipid parameters will be investigated
Detailed Description:
Coronary artery disease CAD also known as coronary heart disease CHD or ischemic heart disease IHD entails a decrease of blood flow to the heart muscle as a result of plaque build-up in the arteries of the heart CAD are among the worlds leading causes of mortality and morbidity following rapid change and advancement of cardiovascular diagnosis and treatment methods
The broad range of lipid molecular species in human plasma and their possible role in human health and disease are topics of great interest Plasma lipidome is now progressively recognized as a potentially important marker of chronic diseases but the exact degree of its contribution to the interindividual phenotypic variation in family studies is uncertain
Associative evidence gathered from plasma lipidomic studies promises vital contributions to biomarker research-an important cornerstone of ongoing efforts to prevent chronic disease The plasma lipidomic profile of humans is associated with various conditions including obesity and disorders of glucose metabolism hypertension cardiovascular diseases
Plasma lipids are solubilized and distributed by associating them with different protein groups Most free fatty acids and associated structures with carboxyl functional groups interact with albumin while more complex lipids are transported and distributed using plasma lipoproteins
Glycerolipids account for a high proportion of total lipids in plasma and include triacylglycerols TAGs diacylglycerols DAGs and ether-linked glycerolipids The total plasma concentration of TAGs allocated between chylomicrons and very-low-density lipoprotein VLDL depends on food intake It is well known that various acquired or secondary risk factors including genetic determinants uncontrolled diabetes mellitus obesity and sedentary lifestyle can cause hypertriglyceridemia a prevalent type of dyslipidemia that is often associated with premature coronary artery disease
D-mannose one of the stereoisomers forms is a structural isomer of D-glucose and commonly found in biological systems but L-mannose is generally not found in biological systems Mannose is transported to mammalian cells by hexose transporters primarily by glucose transporters GLUTs present in the plasma membrane Studies demonstrated that plasma mannose levels in human are associated with incident type 2 diabetes mellitus and cardiocascular diseases On the other hand molecular mechanism by which the pathway is induced is still unclear A recent study showed that human plasma lipidome is pleiotropically associated with the cardiovascular risk factors and death
In mammalian cells mannose is joined in glycolysis and gluconeogenesis pathways catalyzed by mannose phosphate isomerase Mannose is a constituent of normal blood plasma and its concentration is generally elevated in diabetes mellitus and chronic glomerulonephritis However studies showed that fructose and mannose levels are significantly reduced in obese individuals Currently we have reported that elevated baseline mannose in plasma triggered to GLUT4 and Heksokinase-1 HK1 associated with a progressive risk of CAD with time
A cytoplasmic enzyme of lactate dehydrogenase LDH found in all body cells which transfers a hydride from one molecule to another LDH catalyzes the reversible conversion of pyruvate to lactate as a unit of Cori cycle A cardiac marker of LDH is expressed extensively in body cells and a marker of heart failure
The aims of the study were to determine whether modest elevations in plasma mannose concentrations and serum lipids triglyceride low density lipoprotein LDL high density lipoprotein HDL and very-low density lipoprotein VLDL may be associated with a greater incidence of CAD among nondiabetic patients Because hyperglycemia andor hydrophilicity is associated with additional risk factors for CAD including hypertension obesity aging Additionally identifying the molecular and biochemical parameters in plasma associated with risk factors for CAD may have strategic importance in the treatment of these diseases
The patient and control groups will be initially informed about the study and obtained a written consent Patients who underwent angiography at the Department of Cardiology and newly diagnosed as coronary artery disease and who werent on statin treatment will be included in the patient group The control group will be consisted of healthy people with normal coronary arteries angiographically Patients with chronic renal failure chronic liver disease inflammatory disease diabetes insulin resistance major metabolic or endocrine disease will be also excluded For this purpose age and sex matched 120 patients and 120 healthy volunteers will be admitted to study
Blood samples will be collected as described earlier into EDTA and citrate vacutainersTotal cholesterol high-density lipoprotein and triglycerides will be measured by a commercially available enzymatic colorimetric assay Roche BaselSwitzerland Glucose creatinine and the other blood profiles will be determined by standard methodsThe samples will be centrifuged at 400xg for 10 min to separate the serumBiochemical parameters will be measured by using an Abbott ARCHITECH c800Abbott Laboratories USA auto analyser and commercial kits Serum mannose levels will be determined by enzymatic methods using in the ELISA
Kolmogrov-Smirnov test will be used to determine whether the group was parametrically distributed Categorical variables were given as numbers and percentages If the parameters are parametrically distributed continuous variables will be expressed as mean standard deviation if the parameters are non-parametrically distributed continuous variables will be expressed as median IQR When parametric test assumptions will be provided the significance test of difference between two means and one-way analysis of variance will be used to compare independent group differences When parametric test assumptions were not provided Mann-Whitney U test will be used to compare independent group differences The relationships between continuous variables will be analyzed using Spearman correlation analysis chi-square test will be used for analyzing categorical variables Furthermore Receiver Operating Characteristic ROC curve analysis was used for the discriminant performance serum mannose levels under investigation In addition the relationships between continuous variables will be examined by Spearman correlation analysis P 005 will be considered statistically significant in all examinations
The broad range of lipid molecular species in human plasma and their possible role in human health and disease are topics of great interest Plasma lipidome is now progressively recognized as a potentially important marker of chronic diseases but the exact degree of its contribution to the interindividual phenotypic variation in family studies is uncertain
Associative evidence gathered from plasma lipidomic studies promises vital contributions to biomarker research-an important cornerstone of ongoing efforts to prevent chronic disease The plasma lipidomic profile of humans is associated with various conditions including obesity and disorders of glucose metabolism hypertension cardiovascular diseases
Plasma lipids are solubilized and distributed by associating them with different protein groups Most free fatty acids and associated structures with carboxyl functional groups interact with albumin while more complex lipids are transported and distributed using plasma lipoproteins
Glycerolipids account for a high proportion of total lipids in plasma and include triacylglycerols TAGs diacylglycerols DAGs and ether-linked glycerolipids The total plasma concentration of TAGs allocated between chylomicrons and very-low-density lipoprotein VLDL depends on food intake It is well known that various acquired or secondary risk factors including genetic determinants uncontrolled diabetes mellitus obesity and sedentary lifestyle can cause hypertriglyceridemia a prevalent type of dyslipidemia that is often associated with premature coronary artery disease
D-mannose one of the stereoisomers forms is a structural isomer of D-glucose and commonly found in biological systems but L-mannose is generally not found in biological systems Mannose is transported to mammalian cells by hexose transporters primarily by glucose transporters GLUTs present in the plasma membrane Studies demonstrated that plasma mannose levels in human are associated with incident type 2 diabetes mellitus and cardiocascular diseases On the other hand molecular mechanism by which the pathway is induced is still unclear A recent study showed that human plasma lipidome is pleiotropically associated with the cardiovascular risk factors and death
In mammalian cells mannose is joined in glycolysis and gluconeogenesis pathways catalyzed by mannose phosphate isomerase Mannose is a constituent of normal blood plasma and its concentration is generally elevated in diabetes mellitus and chronic glomerulonephritis However studies showed that fructose and mannose levels are significantly reduced in obese individuals Currently we have reported that elevated baseline mannose in plasma triggered to GLUT4 and Heksokinase-1 HK1 associated with a progressive risk of CAD with time
A cytoplasmic enzyme of lactate dehydrogenase LDH found in all body cells which transfers a hydride from one molecule to another LDH catalyzes the reversible conversion of pyruvate to lactate as a unit of Cori cycle A cardiac marker of LDH is expressed extensively in body cells and a marker of heart failure
The aims of the study were to determine whether modest elevations in plasma mannose concentrations and serum lipids triglyceride low density lipoprotein LDL high density lipoprotein HDL and very-low density lipoprotein VLDL may be associated with a greater incidence of CAD among nondiabetic patients Because hyperglycemia andor hydrophilicity is associated with additional risk factors for CAD including hypertension obesity aging Additionally identifying the molecular and biochemical parameters in plasma associated with risk factors for CAD may have strategic importance in the treatment of these diseases
The patient and control groups will be initially informed about the study and obtained a written consent Patients who underwent angiography at the Department of Cardiology and newly diagnosed as coronary artery disease and who werent on statin treatment will be included in the patient group The control group will be consisted of healthy people with normal coronary arteries angiographically Patients with chronic renal failure chronic liver disease inflammatory disease diabetes insulin resistance major metabolic or endocrine disease will be also excluded For this purpose age and sex matched 120 patients and 120 healthy volunteers will be admitted to study
Blood samples will be collected as described earlier into EDTA and citrate vacutainersTotal cholesterol high-density lipoprotein and triglycerides will be measured by a commercially available enzymatic colorimetric assay Roche BaselSwitzerland Glucose creatinine and the other blood profiles will be determined by standard methodsThe samples will be centrifuged at 400xg for 10 min to separate the serumBiochemical parameters will be measured by using an Abbott ARCHITECH c800Abbott Laboratories USA auto analyser and commercial kits Serum mannose levels will be determined by enzymatic methods using in the ELISA
Kolmogrov-Smirnov test will be used to determine whether the group was parametrically distributed Categorical variables were given as numbers and percentages If the parameters are parametrically distributed continuous variables will be expressed as mean standard deviation if the parameters are non-parametrically distributed continuous variables will be expressed as median IQR When parametric test assumptions will be provided the significance test of difference between two means and one-way analysis of variance will be used to compare independent group differences When parametric test assumptions were not provided Mann-Whitney U test will be used to compare independent group differences The relationships between continuous variables will be analyzed using Spearman correlation analysis chi-square test will be used for analyzing categorical variables Furthermore Receiver Operating Characteristic ROC curve analysis was used for the discriminant performance serum mannose levels under investigation In addition the relationships between continuous variables will be examined by Spearman correlation analysis P 005 will be considered statistically significant in all examinations
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None