Ignite Creation Date:
2024-05-06 @ 4:06 PM
Last Modification Date:
2024-10-26 @ 2:03 PM
Study NCT ID:
NCT04871932
Status:
RECRUITING
Last Update Posted:
2021-06-03
First Post:
2021-04-30
Brief Title:
COVID-2019 Vaccine Immune Response Base on Single Cell Multi-Omics
Sponsor:
RenJi Hospital
Organization:
RenJi Hospital
Study Overview
Official Title:
Evolution of Immune System in Healthy Adults After Vaccination of New Coronavirus COVID-2019 Inactivated Vaccine Based on Single Cell Multi-Omics Technologies
Status:
RECRUITING
Status Verified Date:
2021-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
In recent years single-cell high-throughput sequencing technology has developed rapidly and is widely used in research related to the immune system breaking traditional cognition and gaining a new understanding of immune cell classification In particular the emerging single cell RNA sequencing scRNA-seq provides new ideas for the study of cell heterogeneity in multicellular organisms Analyzing the changes in the expression profile of the cell transcriptome at the single-cell level can clearly show the changes in the trajectory of individual cells reveal new cell types and discover the potential functions of immune cells Therefore this study intends to recruit healthy adults and use multi-omics techniques such as single-cell sequencing to systematically classify the peripheral blood mononuclear cells of healthy adults to provide a basis for further disease-related research
Detailed Description:
As an important part of the human body the immune system is closely related to the occurrence of diseases Based on the traditional classification methodology it is mainly divided into two branches innate immunity and adaptive immunity Innate immune cells mainly include monocytes Mono natural killer NK cells and dendritic cells DC The adaptive immune cells mainly include B lymphocytes B and T lymphocytes T Peripheral blood mononuclear cells PBMCs mainly include T cells B cells NK cells Mono cells and DC cells
The proportion of these cell populations varies among individuals Usually in PBMC T lymphocytes account for 45-70 B cells account for 5-15 NK cells account for 5-20 Mono cells account for 10-30 and DC cells account for 1-2 Among them B cells can be divided into transitional naive memory subgroups and plasma cells While T cells are mainly composed of cluster of differentiation 4 CD4 T cells and cluster of differentiation 8 CD8 T cells with the ratio about 21 Whats more CD4 T cells and CD8 T cells can be further divided into naive cells central memory cells in contact with antigen effector memory cells and effector cells Mono cells can be divided into classic monocytes and non-classical cluster of differentiation 16 CD16 pro-inflammatory monocytes DC cells include plasmacytic dendritic cells pDC and myeloid dendritic cells mDC
In recent years scRNA-seq has developed rapidly and is widely used in research related to the immune system breaking traditional cognition and gaining a new understanding of immune cell classification In particular the emerging scRNA-seq provides new ideas for the study of cell heterogeneity in multicellular organisms Analyzing the changes in the expression profile of the cell transcriptome at the single-cell level can clearly show the changes in the trajectory of individual cells reveal new cell types and discover the potential functions of immune cells
Adults have a relatively stable immune system with little interference from the external environment Therefore this study intends to recruit healthy adults and use multi-omics techniques such as scRNA-seq to systematically classify the PBMCs of healthy adults to provide a basis for further disease-related research
The proportion of these cell populations varies among individuals Usually in PBMC T lymphocytes account for 45-70 B cells account for 5-15 NK cells account for 5-20 Mono cells account for 10-30 and DC cells account for 1-2 Among them B cells can be divided into transitional naive memory subgroups and plasma cells While T cells are mainly composed of cluster of differentiation 4 CD4 T cells and cluster of differentiation 8 CD8 T cells with the ratio about 21 Whats more CD4 T cells and CD8 T cells can be further divided into naive cells central memory cells in contact with antigen effector memory cells and effector cells Mono cells can be divided into classic monocytes and non-classical cluster of differentiation 16 CD16 pro-inflammatory monocytes DC cells include plasmacytic dendritic cells pDC and myeloid dendritic cells mDC
In recent years scRNA-seq has developed rapidly and is widely used in research related to the immune system breaking traditional cognition and gaining a new understanding of immune cell classification In particular the emerging scRNA-seq provides new ideas for the study of cell heterogeneity in multicellular organisms Analyzing the changes in the expression profile of the cell transcriptome at the single-cell level can clearly show the changes in the trajectory of individual cells reveal new cell types and discover the potential functions of immune cells
Adults have a relatively stable immune system with little interference from the external environment Therefore this study intends to recruit healthy adults and use multi-omics techniques such as scRNA-seq to systematically classify the PBMCs of healthy adults to provide a basis for further disease-related research
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None