Ignite Creation Date:
2024-05-06 @ 4:04 PM
Last Modification Date:
2024-10-26 @ 2:03 PM
Study NCT ID:
NCT04865198
Status:
COMPLETED
Last Update Posted:
2022-07-26
First Post:
2021-04-20
Brief Title:
Neuralized1 and RGS14 Genes
Sponsor:
TC Erciyes University
Organization:
TC Erciyes University
Study Overview
Official Title:
Role of Neuralized1 and RGS14 Genes With ASD Patients
Status:
COMPLETED
Status Verified Date:
2022-07
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Autism is a broad spectrum neurodevelopmental disease Some individuals with ADS by high cognitive functions are diagnosed with High Functioning Autism HFA In some studies it has been shown that NEURL1 gene increases learning and memory and RGS14 gene is suppressed them We aimed to evaluate the differences between the expression levels of these genes between ASD HFA and healthy controls and the role of these genes in the pathogenesis of ASD Patients with 20 ASD and 20 HFA and 20 healthy controls compatible with patient ages were included in this study Expression of NEURL-1 and RGS14 genes was evaluated by quantitative Real Time PCR qRT-PCR
Detailed Description:
ASD is a neurological disease starting in the early stages of life and is characterized by cognitive and behavioral disorders Ansel et al 2008Alvares et al 2020 It is considered that the etiology of ASD stems from genetic epigenetic and environmental factors however it has not yet been definitively clarifiedIto et al 2017
we aimed to evaluate the differences between the expression levels of these genes between ASD HFA and healthy controls and the role of these genes in the pathogenesis of ASD
Method
Patients with ASD n20 and HFA n20 and healthy controls n20 compatible with patient ages were included in this study Clinical evaluations of the patients were made and classification was made in accordance with DSM-IV diagnostic criteria
High Pure RNA Isolation Kit Roche Diagnostic Version 12 Germany was used for RNA isolation cDNA synthesis was performed from these RNAs with the ranscriptor High Fidelity cDNA Synthesis Kit Roche Diagnostics GmbH Mannheim
qRT-PCR was performed using the LightCycler480 Real Time Ready Assay Master Probe Kit Roche Diagnostics GmbH MannheimThe incubation was made with the PCR device program for 10 minutes at 95oC for 45 cycles for 10 sec at 95oC and for 60 sec at 60oC The Ct values were obtained from the Light Cycler 480 Software Program and both genes were analyzed separately The comparative CT method 2-ΔΔCT was used to determine the relative quantification of target genes normalized to a housekeeping gene β-actin
Statisticaly
The results of the experiments were evaluated using R 311 wwwr-projectorg and Chi-Square Tests Mann-Whitney U-Test Kruskal-Wallis H-Tests The P005 level was taken as significant
we aimed to evaluate the differences between the expression levels of these genes between ASD HFA and healthy controls and the role of these genes in the pathogenesis of ASD
Method
Patients with ASD n20 and HFA n20 and healthy controls n20 compatible with patient ages were included in this study Clinical evaluations of the patients were made and classification was made in accordance with DSM-IV diagnostic criteria
High Pure RNA Isolation Kit Roche Diagnostic Version 12 Germany was used for RNA isolation cDNA synthesis was performed from these RNAs with the ranscriptor High Fidelity cDNA Synthesis Kit Roche Diagnostics GmbH Mannheim
qRT-PCR was performed using the LightCycler480 Real Time Ready Assay Master Probe Kit Roche Diagnostics GmbH MannheimThe incubation was made with the PCR device program for 10 minutes at 95oC for 45 cycles for 10 sec at 95oC and for 60 sec at 60oC The Ct values were obtained from the Light Cycler 480 Software Program and both genes were analyzed separately The comparative CT method 2-ΔΔCT was used to determine the relative quantification of target genes normalized to a housekeeping gene β-actin
Statisticaly
The results of the experiments were evaluated using R 311 wwwr-projectorg and Chi-Square Tests Mann-Whitney U-Test Kruskal-Wallis H-Tests The P005 level was taken as significant
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None