Ignite Creation Date:
2024-05-06 @ 3:48 PM
Last Modification Date:
2024-10-26 @ 1:57 PM
Study NCT ID:
NCT04761081
Status:
COMPLETED
Last Update Posted:
2022-04-08
First Post:
2021-02-09
Brief Title:
Is Physical Activity Obesity and Ethnicity Associated With the Tethering and Migration of Pro-inflammatory Monocytes
Sponsor:
Loughborough University
Organization:
Loughborough University
Study Overview
Official Title:
Is Physical Activity Associated With the Tethering and Migration of Pro-inflammatory Monocytes in White European and South Asian Males With and Without Central Obesity
Status:
COMPLETED
Status Verified Date:
2022-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Being south Asian or centrally obese may be associated with an increased risk of inflammation The investigators are seeking to investigate whether this is the case by recruiting white European and south Asian men who are lean or have central obesity Further the investigators wish to investigate whether physical activity influences the associations
Detailed Description:
Central obesity is associated with an increased risk of cardiovascular disease Further south Asians have been shown to be at an increased risk of cardiovascular disease compared to white Europeans
Cardiovascular disease is underpinned by inflammation Evidence suggests that people with obesity have a more pro-inflammatory and pro-migratory monocyte profile compared with individuals who are lean The excessive monocyte migration contributes to metabolic dysfunction over time increasing the risk of chronic disease However there is no evidence in south Asians
One modifiable risk factor which may be able to influence this is physical inactivity with higher levels of physical activity being associated with reduced inflammation However although south Asians are more at risk of cardiovascular disease than white Europeans evidence suggests south Asians are also less physically active than white Europeans
The investigators are looking to recruit south Asian and white European men who are lean or have central obesity to investigate 1 is there an association between ethnicity and the tethering and migration of pro-inflammatory monocytes 2 is there an association between central obesity and the tethering and migration of pro-inflammatory monocytes and is there an interaction with ethnicity 3 do higher levels of physical activity influence the tethering and migration of pro-inflammatory monocytes and is this influenced by ethnicity or central obesity
To investigate this the investigators are looking to recruit south Asian and white European men who are either centrally obese or lean The investigators require 1 blood sample and the participants to wear an activity monitor for 7 days
Peripheral blood mononuclear cells PBMCs will be isolated from the whole blood sample Then the investigators will quantify the migratory capacity of PBMCs to a fixed chemokine gradient over time Further the investigators will phenotype the monocytes to indicate the characteristics of the monocytes that migrate towards the chemokine mix
The activity monitor will quantify habitual physical activity which will be used in the statistical analyses to investigate whether physical activity may influence the response
It is important to investigate as it will further scientific knowledge on the underpinnings of chronic disease and enable a better understanding on the role of physical activity to potentially reduce the risk
Cardiovascular disease is underpinned by inflammation Evidence suggests that people with obesity have a more pro-inflammatory and pro-migratory monocyte profile compared with individuals who are lean The excessive monocyte migration contributes to metabolic dysfunction over time increasing the risk of chronic disease However there is no evidence in south Asians
One modifiable risk factor which may be able to influence this is physical inactivity with higher levels of physical activity being associated with reduced inflammation However although south Asians are more at risk of cardiovascular disease than white Europeans evidence suggests south Asians are also less physically active than white Europeans
The investigators are looking to recruit south Asian and white European men who are lean or have central obesity to investigate 1 is there an association between ethnicity and the tethering and migration of pro-inflammatory monocytes 2 is there an association between central obesity and the tethering and migration of pro-inflammatory monocytes and is there an interaction with ethnicity 3 do higher levels of physical activity influence the tethering and migration of pro-inflammatory monocytes and is this influenced by ethnicity or central obesity
To investigate this the investigators are looking to recruit south Asian and white European men who are either centrally obese or lean The investigators require 1 blood sample and the participants to wear an activity monitor for 7 days
Peripheral blood mononuclear cells PBMCs will be isolated from the whole blood sample Then the investigators will quantify the migratory capacity of PBMCs to a fixed chemokine gradient over time Further the investigators will phenotype the monocytes to indicate the characteristics of the monocytes that migrate towards the chemokine mix
The activity monitor will quantify habitual physical activity which will be used in the statistical analyses to investigate whether physical activity may influence the response
It is important to investigate as it will further scientific knowledge on the underpinnings of chronic disease and enable a better understanding on the role of physical activity to potentially reduce the risk
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None