Ignite Creation Date:
2024-05-06 @ 3:46 PM
Last Modification Date:
2024-10-26 @ 1:56 PM
Study NCT ID:
NCT04753021
Status:
COMPLETED
Last Update Posted:
2023-04-12
First Post:
2021-02-04
Brief Title:
Dose-response Relationship Between Exercise and Health Outcomes
Sponsor:
The Swedish School of Sport and Health Sciences
Organization:
The Swedish School of Sport and Health Sciences
Study Overview
Official Title:
Dose-response Relationship Between Exercise and Health Outcomes
Status:
COMPLETED
Status Verified Date:
2023-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
In this study 11 healthy volunteers will perform high intensity exercise training during 4 weeks The exercise frequency will increase progressively during the first three weeks followed by a fourth week with lower exercise frequency 5 skeletal muscle biopsies will be donated throughout the study and 4 oral glucose tolerance tests will be performed after each training period
Detailed Description:
11 healthy and recreational active subjects will be recruited to the study For inclusion and assessment of baseline physiological characteristics during cycling a pre-test will be performed on a cycle ergometer and the submaximal and maximal response to exercise will be determined by measurement of respiratory gas exchange The physiological response to submaximal work rate will be comprised of a series of five minutes long intervals separated with one minutes of rest with assessment of capillary blood lactate and glucose at the end of each workload All physiological tests and training HII sessions will be carefully supervised by trained personnel The intervention period will include 14 HIIT-sessions in total all performed at the laboratory under close surveillance and careful monitoring of power output and heart rate During HIIT sessions VO2 CO2 blood glucose lactate and rating of perceived exhaustion BORG will be measured
Energy intake will be standardized for each subject during the day of test sessions After each HIIT-session a recovery drink containing 1 g kg-1 bw of carbohydrates and 025 g kg-1 bw of protein will be ingested by the subjects After HIIT sessions subjects will be supplied with an evening meal consisting of 74 g carbohydrates 38 g protein and 26 g fat to be consumed two hours post exercise Subjects will thereafter remain fasted until the next morning for an oral glucose tolerance test OGTT and donation of a muscle biopsy
All biopsies will be collected in fasted state in early morning following 14 hour of rest after the last HIIT-session Biopsies will be taken from vastus lateralis in alternating leg in randomized order
Each subject will complete four OGTTs throughout the study Resting metabolic rate RMR will be measured in supine position by gas exchange measurement using mixing chamber method Oxycon Pro attached to a ventilated hood Thereafter the muscle biopsy will be taken and a catheter will be inserted into the antecubital vein of the forearm After 10 minutes of passive rest 75 mg glucose dissolved in 300 ml water will be ingested and at 0 15 30 45 60 75 90 105- and 120-minutes after glucose intake a venous blood sample will be taken and instantly analyzed for glucose with enzymatic method in the Biosen C-Line Clinic Additional RMR measurements will be performed at 40 and 115 minutes after glucose ingestion
Mitochondria will be isolated from fresh skeletal muscle biopsies Mitochondrial respiration and H2O2 emission will be measured using a two-channel high-resolution respirometer with an attached fluorescent probe Oxygraph-2k Oroboros Instruments Corporation Innsbruck Austria
Energy intake will be standardized for each subject during the day of test sessions After each HIIT-session a recovery drink containing 1 g kg-1 bw of carbohydrates and 025 g kg-1 bw of protein will be ingested by the subjects After HIIT sessions subjects will be supplied with an evening meal consisting of 74 g carbohydrates 38 g protein and 26 g fat to be consumed two hours post exercise Subjects will thereafter remain fasted until the next morning for an oral glucose tolerance test OGTT and donation of a muscle biopsy
All biopsies will be collected in fasted state in early morning following 14 hour of rest after the last HIIT-session Biopsies will be taken from vastus lateralis in alternating leg in randomized order
Each subject will complete four OGTTs throughout the study Resting metabolic rate RMR will be measured in supine position by gas exchange measurement using mixing chamber method Oxycon Pro attached to a ventilated hood Thereafter the muscle biopsy will be taken and a catheter will be inserted into the antecubital vein of the forearm After 10 minutes of passive rest 75 mg glucose dissolved in 300 ml water will be ingested and at 0 15 30 45 60 75 90 105- and 120-minutes after glucose intake a venous blood sample will be taken and instantly analyzed for glucose with enzymatic method in the Biosen C-Line Clinic Additional RMR measurements will be performed at 40 and 115 minutes after glucose ingestion
Mitochondria will be isolated from fresh skeletal muscle biopsies Mitochondrial respiration and H2O2 emission will be measured using a two-channel high-resolution respirometer with an attached fluorescent probe Oxygraph-2k Oroboros Instruments Corporation Innsbruck Austria
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None