Ignite Creation Date:
2024-05-06 @ 3:23 PM
Last Modification Date:
2024-10-26 @ 1:48 PM
Study NCT ID:
NCT04615364
Status:
RECRUITING
Last Update Posted:
2024-05-23
First Post:
2020-10-29
Brief Title:
Genetic Evolution of Arboviruses in New Caledonia Between 1995 and 2024 and Impact of Wolbachia
Sponsor:
Institut Pasteur
Organization:
Institut Pasteur
Study Overview
Official Title:
Genetic Evolution of Arboviruses in New Caledonia Between 1995 and 2024 and Impact of Wolbachia
Status:
RECRUITING
Status Verified Date:
2024-05
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
DENWOLUTION
Brief Summary:
Arboviruses diseases transmitted to humans by the bite of an insect vector are a major public health problem especially in tropical and sub-tropical countries A promising strategy aimed at blocking the circulation of arboviruses is to release Aedes aegypti mosquitoes carrying the endosymbiotic bacterium Wolbachia In 2019 the Wolbachia strategy was implemented in Nouméa as part of the World Mosquito Program This intervention will modify the epidemiological profile of arboviruses in New Caledonia
Epidemiological surveillance of arboviruses requires molecular characterization of the virus contained in the serum obtained from the blood collected from patients This molecular characterization by RNA isolation techniques RT-qPCR monitoring and sequencing allows the construction of phylogenetic trees
In the context of the implementation of the World Mosquito Program in Nouméa the investigators plan to follow the molecular evolution of arboviruses over the period preceding the releases of mosquitoes carrying Wolbachia from 1995 to 2019 then over a period of 5 years following the releases
At the same time the virus can be isolated by cell culture techniques and in vitro infections allowing its study in vitro in cells or in vivo in mosquitoes This study allows us to measure the impact of the Wolbachia strategy on the evolution of the viruss ability to replicate in cells in the presence of Wolbachia and to be transmitted by the mosquito
Epidemiological surveillance of arboviruses requires molecular characterization of the virus contained in the serum obtained from the blood collected from patients This molecular characterization by RNA isolation techniques RT-qPCR monitoring and sequencing allows the construction of phylogenetic trees
In the context of the implementation of the World Mosquito Program in Nouméa the investigators plan to follow the molecular evolution of arboviruses over the period preceding the releases of mosquitoes carrying Wolbachia from 1995 to 2019 then over a period of 5 years following the releases
At the same time the virus can be isolated by cell culture techniques and in vitro infections allowing its study in vitro in cells or in vivo in mosquitoes This study allows us to measure the impact of the Wolbachia strategy on the evolution of the viruss ability to replicate in cells in the presence of Wolbachia and to be transmitted by the mosquito
Detailed Description:
Collection of blood samples from patients with arbovirus infection
This study is a non-interventional study with retrospective part
This study will improve
the assessment of the molecular evolution of arboviruses in the period preceding the implementation of the Wolbachia strategy
the knowledge of the impact of Wolbachia on the diversity and molecular evolution of arboviruses
the understanding of arbovirus transmission and replication mechanisms in the presence of Wolbachia
This study is a non-interventional study with retrospective part
This study will improve
the assessment of the molecular evolution of arboviruses in the period preceding the implementation of the Wolbachia strategy
the knowledge of the impact of Wolbachia on the diversity and molecular evolution of arboviruses
the understanding of arbovirus transmission and replication mechanisms in the presence of Wolbachia
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None