Ignite Creation Date:
2024-05-05 @ 5:11 PM
Last Modification Date:
2024-10-26 @ 9:29 AM
Study NCT ID:
NCT00401297
Status:
COMPLETED
Last Update Posted:
2020-03-30
First Post:
2006-11-16
Brief Title:
Th1Th2 Polarization and Linkage to L Viannia Infection Outcomes
Sponsor:
Yale University
Organization:
Yale University
Study Overview
Official Title:
Intervenable Host- Leishmania Vianna Interactions Project 3 Immune and Inflammatory Responses in L Viannia Infection Aim 1 To Determine if Th1Th2 Polarization Occurs and is Linked to the Outcome of Infection by L Viannia
Status:
COMPLETED
Status Verified Date:
2011-08
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The purpose of this study is to determine what types of cells participate in the defense of humans against Leishmania skin parasites People 18-70 years of age who have leishmaniasis have healed leishmanial lesions or are healthy are being invited to participate in this study Approximately 150 people will participate in the study Participants will be asked to provide some general information about themselves and about skin sores if they have any A skin test will be performed and a blood sample will be obtained This study involves up to 3 visits the first visit will last up to 5 hours and the second visit will last for 30 minutes The third visit may be scheduled within 3 days after the second visit
Detailed Description:
The purpose of this study is to determine if Th1Th2 polarization occurs and is linked to the outcome of infection by L Viannia asymptomatic chronic or recurrent infection This protocol is the first part of a series of studies that includes protocols 05-0139 and 06-0009 Specific objectives include defining the expression of human inflammatory cytokines in the ex vivo recall response to live Leishmania for PBMCs of individuals presenting different clinical phenotypes at transcriptional translational and secretion level and assessing in situ and circulating cell populations from active chronic and recurrent patients with respect to differentiation activation and co-activation markers Patients males and females aged 18-70 years with cutaneous leishmaniasis diagnosed in either Cali CIDEIM andor Tumaco San Andrés Hospital on the Colombian Pacific Coast andor Chaparral Tolima San Juan Bautista Hospital will be invited to participate in the study Both historic specific objective 1 and active specific objective 2 patients will be enrolled Asymptomatic individuals and healthy donors controls will also be invited to participate Pregnant or breastfeeding women will not be enrolled A total of 150 participants will be enrolled For specific objective 1 participants will be asked to provide relevant demographic clinical and epidemiologic information that will be recorded in a form designed for this purpose They will also provide blood samples that will be analyzed to determine and compare the expression of human inflammatory cytokines in the ex vivo recall response to live Leishmania of PBMCs between the four groups Specific objective 2 will be developed in CIDEIM-Cali only Participants will be asked to provide skin biopsies in addition to blood samples and relevant information They will also undergo a skin blister procedure Samples will be analyzed to characterize and compare in situ and circulating cell populations between the groups Up to three visits are planned for this objective The first visit will have a duration of up to 5 hours and the second one will have a duration of 30 minutes A third visit may be programmed 48h-72h after the second visit for participants undergoing biopsyskin blister procedure in order to evaluate possible bacterial superinfection and prevent complications This visit will have a duration of 20 minutes All visits will take place in a period of 4-5 days In order to define the expression of human inflammatory cytokines in the ex vivo recall response to live Leishmania for PBMCs of individuals presenting different clinical phenotypes at transcriptional translational and secretion level the following variables will be measured and compared among the different groups levels of expression of transcription factors in cells that have and have not been stimulated by live Leishmania cytokine concentration at the transcriptional and secretion levels in cells that have and have not been stimulated by live Leishmania and percentage of specific PBMCs producing intracellular cytokines at translational level In order to assess in situ biopsy and blister and circulating cell populations from active chronic and recurrent patients with respect to differentiation activation and co-activation markers the following variables will be measured and compared among the different groups percentage of infiltrating leukocytes in blister fluid and biopsy and circulating leukocytes in blood expressing specific differentiation activation and co-stimulation markers mean fluorescence intensities of cells in blood blister fluid and biopsy expressing specific differentiation activation and co-stimulation markers percentage of specific cells from blister fluid biopsies and blood producing intracellular cytokines IFN-gamma IL-10 IL-4 and IL-13 and cytokine concentration in blister fluid
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| U19AI065866 | NIH | None | httpsreporternihgovquickSearchU19AI065866 |