Ignite Creation Date:
2024-05-05 @ 5:08 PM
Last Modification Date:
2024-10-26 @ 9:28 AM
Study NCT ID:
NCT00396695
Status:
UNKNOWN
Last Update Posted:
2006-11-07
First Post:
2006-11-06
Brief Title:
Neuroimmunological Model of Traumatic Memory
Sponsor:
Ziv Hospital
Organization:
Ziv Hospital
Study Overview
Official Title:
Neuroimmunological Model of Traumatic Memory
Status:
UNKNOWN
Status Verified Date:
2006-11
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Traumatic events may lead to strong emotional episodic memories common in Post- Traumatic Stress DisorderPTSD Intense affect may inhibit efficacy of glutamatergic neurotransmission in two particular areas of the limbic system that have been implicated in the processing of emotionally charged memories the amygdala and the hippocampus12
Dysfunction of glutamatergic neurotransmission is associated with disbalance of long-term potentiation LTP and long-term depression LTD- two underlying mechanisms that cooperate to achieve synaptic plasticity and its expressations- learning and memory3 LTP- the long lasting enhancement of synaptic function includes changes in the amount of neurotransmitter glutamate released into a synapse changes in the levels of key proteins in synapses protein phosphorylation and changes the density of receptors on their synaptic membranes LTD is the inverse of LTP a long lasting reduction in synaptic transmission 4 Interactions among the different forms of plasticity underlie different forms of memories Normally these mechanisms are balanced
In the current literature there is data that a class I major histocompatibility complex MHC class I molecules known to be important for immune responses to antigen are expressed also by neurons that undergo activity-dependence long-term structural and synaptic modifications 5 The brain produces its own immune molecules the proteins MHC class I and CD3-zeta a component of receptors for MHC class I In the immune system the two proteins act as part of a lock and key system to recognize and get rid of the bodys foreign invaders The CD3-zeta polypeptide is component of the T cell antigen receptor TCR which contribute to its efficient cell surface expression and account for part of its transducing capability 6
In the brain they may be part of a signaling system that recognizes and eliminates inappropriate neural connections Expression of MHC class I is regulated by the naturally occurring electrical activity and sensitive to both natural and pathological changes in the activity Electrical activity of neurons drives to an establishment of the final pattern of connection Changes in the strength of individual synapses such as potention and depression leads to stabilization and withdrawal respectively of the affected connections There are data that in mice with deficiency of MHC class I and CD3-zeta the LTP in the hippocampus is enhanced significantly and LTD is absent Thus MHC class I is crucial for translating activity into changes in synaptic strength and neuronal connectivity in vivo He required for normal activity dependent potentiation depression removal of inappropriate connection and responding to injury in the CNS 6
Glutamate receptors play critical roles in LTPLTD mechanisms Some researchers consider that a key role in pathogenesis of PTSD is being played by excessive excitation of NMDA-receptors in limbic system structures 1 The existing data allows to assume that equation of plasticity mechanisms depends on mutual relations between the MHC class I and glutamate receptors
T-cells like neurons express high levels of glutamate receptors that are identical to the brain glutamate receptors Presence of ionotropic and metabotropic glutamate receptors in membranes of lymphocytes makes them sensitive to the same alarm molecules which operate neuronal activity Glutamate by itself triggers several T-cell activation which differs quantitatively or qualitatively from that ones triggered by classical T-cell activators like antigens7 There are data about influence of T cell receptor-CD3 complex- on the expression of T-cells glutamate receptors 8 It is possible that the key roles in this function play CD3-zeta
Dysfunction of glutamatergic neurotransmission is associated with disbalance of long-term potentiation LTP and long-term depression LTD- two underlying mechanisms that cooperate to achieve synaptic plasticity and its expressations- learning and memory3 LTP- the long lasting enhancement of synaptic function includes changes in the amount of neurotransmitter glutamate released into a synapse changes in the levels of key proteins in synapses protein phosphorylation and changes the density of receptors on their synaptic membranes LTD is the inverse of LTP a long lasting reduction in synaptic transmission 4 Interactions among the different forms of plasticity underlie different forms of memories Normally these mechanisms are balanced
In the current literature there is data that a class I major histocompatibility complex MHC class I molecules known to be important for immune responses to antigen are expressed also by neurons that undergo activity-dependence long-term structural and synaptic modifications 5 The brain produces its own immune molecules the proteins MHC class I and CD3-zeta a component of receptors for MHC class I In the immune system the two proteins act as part of a lock and key system to recognize and get rid of the bodys foreign invaders The CD3-zeta polypeptide is component of the T cell antigen receptor TCR which contribute to its efficient cell surface expression and account for part of its transducing capability 6
In the brain they may be part of a signaling system that recognizes and eliminates inappropriate neural connections Expression of MHC class I is regulated by the naturally occurring electrical activity and sensitive to both natural and pathological changes in the activity Electrical activity of neurons drives to an establishment of the final pattern of connection Changes in the strength of individual synapses such as potention and depression leads to stabilization and withdrawal respectively of the affected connections There are data that in mice with deficiency of MHC class I and CD3-zeta the LTP in the hippocampus is enhanced significantly and LTD is absent Thus MHC class I is crucial for translating activity into changes in synaptic strength and neuronal connectivity in vivo He required for normal activity dependent potentiation depression removal of inappropriate connection and responding to injury in the CNS 6
Glutamate receptors play critical roles in LTPLTD mechanisms Some researchers consider that a key role in pathogenesis of PTSD is being played by excessive excitation of NMDA-receptors in limbic system structures 1 The existing data allows to assume that equation of plasticity mechanisms depends on mutual relations between the MHC class I and glutamate receptors
T-cells like neurons express high levels of glutamate receptors that are identical to the brain glutamate receptors Presence of ionotropic and metabotropic glutamate receptors in membranes of lymphocytes makes them sensitive to the same alarm molecules which operate neuronal activity Glutamate by itself triggers several T-cell activation which differs quantitatively or qualitatively from that ones triggered by classical T-cell activators like antigens7 There are data about influence of T cell receptor-CD3 complex- on the expression of T-cells glutamate receptors 8 It is possible that the key roles in this function play CD3-zeta
Detailed Description:
Working hypothesis and aims
Trauma-related LTPLTD disbalance in favor of potentaion and excessive excitation of glutamate receptors generated when arousing experiences occur in conjunction with memory-related activation of the hippocampus and amigdala Traumatic memories connected to the amplification of mechanism LTP when new information is unable to induce reliable LTD which reverses synaptic plasticity formed during previous emotional learning experiences
MHC class I and CD3-zeta play a key role in these changes We assume that the composition of glutamate receptors and CD3-zeta expression in T-cells can be used as the model reflecting for LTPLTD balance in CNS
The work includes the learning of following parameters
1 The Expression in T-cells glutamate receptors CD3-zeta
2 Glutamate level in plasma
3 Cytokines levels in plasma
1 interferon-γ that can induce some components of class I MHC 9
2 tumor necrosis factor-α that influences the AMPAR expression and plays a role in LTP reduction 10
Groups of 20 people each will make patients with a priori various degree of LTPLTD balance healthy people and PTSD patients
People suffering PTSD frequently have conditions when they again brightly and deeply experience event injuring them The memoirs painted by painful emotions seize attention of the person and then it seems to him as if he again experiences injuring event and sees it as real
We assume that it is connected to the unable to induce reliable LTD and that the composition of glutamate receptors and CD3-zeta expression in T-cells such people should differ from healthy
Used method enzyme- linked immunosorbent assay ELISA FACS
Trauma-related LTPLTD disbalance in favor of potentaion and excessive excitation of glutamate receptors generated when arousing experiences occur in conjunction with memory-related activation of the hippocampus and amigdala Traumatic memories connected to the amplification of mechanism LTP when new information is unable to induce reliable LTD which reverses synaptic plasticity formed during previous emotional learning experiences
MHC class I and CD3-zeta play a key role in these changes We assume that the composition of glutamate receptors and CD3-zeta expression in T-cells can be used as the model reflecting for LTPLTD balance in CNS
The work includes the learning of following parameters
1 The Expression in T-cells glutamate receptors CD3-zeta
2 Glutamate level in plasma
3 Cytokines levels in plasma
1 interferon-γ that can induce some components of class I MHC 9
2 tumor necrosis factor-α that influences the AMPAR expression and plays a role in LTP reduction 10
Groups of 20 people each will make patients with a priori various degree of LTPLTD balance healthy people and PTSD patients
People suffering PTSD frequently have conditions when they again brightly and deeply experience event injuring them The memoirs painted by painful emotions seize attention of the person and then it seems to him as if he again experiences injuring event and sees it as real
We assume that it is connected to the unable to induce reliable LTD and that the composition of glutamate receptors and CD3-zeta expression in T-cells such people should differ from healthy
Used method enzyme- linked immunosorbent assay ELISA FACS
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None