Ignite Creation Date:
2024-05-06 @ 2:35 PM
Last Modification Date:
2024-10-26 @ 1:33 PM
Study NCT ID:
NCT04365140
Status:
COMPLETED
Last Update Posted:
2020-04-29
First Post:
2020-04-23
Brief Title:
MicroRNA-126 and Its Target VCAM-1Dermatitis to Nickel
Sponsor:
Universidad Autonoma de San Luis Potosí
Organization:
Universidad Autonoma de San Luis Potosí
Study Overview
Official Title:
Alteration of the microRNA-126 Expression a Possible Mechanism in Allergic Contact Dermatitis to Nickel
Status:
COMPLETED
Status Verified Date:
2020-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Background Allergic Contact Dermatitis ACD is an inflammatory skin disease mediated by direct contact with allergens as nickel the most common allergen that may be related with epigenetic changes
Objective Evaluate the miR-126 expression and its target VCAM-1 in the skin of patients with ACD to nickel
Methods Fifteen patients with positive patch test to nickel were included and the expression of miR-126 and VCAM-1 was evaluated by RT-qPCR
Objective Evaluate the miR-126 expression and its target VCAM-1 in the skin of patients with ACD to nickel
Methods Fifteen patients with positive patch test to nickel were included and the expression of miR-126 and VCAM-1 was evaluated by RT-qPCR
Detailed Description:
Patients that went to the dermatology and outpatient department of the Central Hospital Dr Ignacio Morones Prieto with a suspected diagnosis of nickel allergic contact dermatitis were invited to participate in this study Later the objective and methodology of the study were explained to them as well as the advantages of participating and the possible undesirable effects All patients signed the informed consent previous perfom the medical history After the epicutaneous patch test was performed and patients were divided according to the result them Finally under local anesthesia two 3-mm punch biopsies from lesional positive parch test site and adjacent non lesional skin 10 cm apart were performed Tissues for reverse transcriptase real-time polymerase chain reaction RT-qPCR analyses were stored frozen in sterile tubes at 80ºC until processing
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None