Ignite Creation Date:
2024-05-06 @ 2:18 PM
Last Modification Date:
2024-10-26 @ 1:28 PM
Study NCT ID:
NCT04279327
Status:
UNKNOWN
Last Update Posted:
2020-07-08
First Post:
2020-02-19
Brief Title:
Cell Block Immunohistochemistry in Effusion Cytology
Sponsor:
Assiut University
Organization:
Assiut University
Study Overview
Official Title:
Diagnostic Value of Cell Block Immunohistochemistry in Effusion Cytology
Status:
UNKNOWN
Status Verified Date:
2020-07
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Cytology is usually the first step in investigating serous effusions to either detect or exclude an underlying malignancy This study will try to answer the need for improved diagnostic yield of cytologic examination by cell block technique and immunohistochemical testing of three markers which are EZH2 Claudin-4 and MOC-31
Detailed Description:
This is a prospective study that will be conducted at the pathology lab of Assiut University Hospital The study will include effusion specimens pleural peritoneal pericardial and the aspirated fluid will be examined for physical characters eg color and appearance
The sample will be divided into 2 equal parts one for conventional smear preparation and the other for cell block A minimum of 2 thin smears will be prepared and stained with Papanicolou stain
Cell block sections of 4-6 μ thickness will be prepared and stained with the hematoxylin and eosin stain HE
For immunohistochemistry cell blocks will be used Expression of claudin 4 EZH2 and MOC-31 will be evaluated in benign and malignant cells by using immunoperoxidase technique
The sample will be divided into 2 equal parts one for conventional smear preparation and the other for cell block A minimum of 2 thin smears will be prepared and stained with Papanicolou stain
Cell block sections of 4-6 μ thickness will be prepared and stained with the hematoxylin and eosin stain HE
For immunohistochemistry cell blocks will be used Expression of claudin 4 EZH2 and MOC-31 will be evaluated in benign and malignant cells by using immunoperoxidase technique
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None